Gene affecting pathogenicity and generation of conidium and originated from cabbage fusarium wilt bacteria and application of same

A technology of cabbage Fusarium wilt and conidia, which can be applied in application, genetic engineering, plant genetic improvement and other directions, and can solve the problems of lack of gene cloning and molecular mechanism content.

Active Publication Date: 2015-02-25
京科智宝(北京)农业科技有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are relatively few reports on the conidia formation and variation of Fusarium wilt, and even if there are reports, there is generally no content about gene cloning and molecular mechanism

Method used

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  • Gene affecting pathogenicity and generation of conidium and originated from cabbage fusarium wilt bacteria and application of same
  • Gene affecting pathogenicity and generation of conidium and originated from cabbage fusarium wilt bacteria and application of same
  • Gene affecting pathogenicity and generation of conidium and originated from cabbage fusarium wilt bacteria and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Obtaining of proteins and their coding genes that affect conidia production and pathogenicity derived from Fusarium oxysporum

[0039] 1. Obtaining mutant strains

[0040] Fusarium oxysporum f.sp.conglutinans strain JZB310079 (Li Lingli et al., Optimization of the preparation and regeneration conditions of Fusarium oxysporum f.sp.conglutinans), Chinese Agricultural Science Bulletin, 2011,27(10):203-207, public Obtained from Beijing Academy of Agriculture and Forestry Sciences) is a wild strain, prepared gene insertion mutant library, and screened mutants.

[0041] First, the gene insertion mutant library was constructed by the method of REMI transformation, and then the mutant strains with weakened pathogenicity were screened out by comparing with the infectivity of the wild type, and the mutant strains were found by Southern blot analysis of the genomic DNA of the mutant strains. The mutant strain is a single point insertion, that is, a single gene mutation. The ...

Embodiment 2

[0047] Example 2: Functional verification of proteins and their coding genes that affect conidia production and pathogenicity derived from Fusarium oxysporum

[0048] The demonstration of the role of FOG1 in the production of conidia by Fusarium wilt of Brassica oleracea includes complementation experiments and gene replacement experiments. This part of the content includes the construction of complementary vectors and gene replacement vectors and the introduction of the above two types of vectors into Fusarium oxysporum to obtain corresponding transformants. The construction of a complementary vector refers to linking a DNA fragment containing the full-length functional sequence of FOG1 (nucleotides 1035-4250 of sequence 2 in the sequence list) to a vector with a neomycin resistance gene. The Fusarium wilt receptor strain introduced by the complementary vector is a mutant strain. The construction of gene replacement vector refers to linking a piece of DNA sequence on both sides...

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Abstract

The invention discloses a gene affecting pathogenicity and generation of conidium and originated from cabbage wither disease bacteria and the application of the same. The protein is an amino acid residue sequence with SEQ ID No.1 in the sequence table. Trans-gene tests have shown that the gene has the function of affecting pathogenicity of cabbage wither fusarium wilt bacteria and generation of conidium, and the gene can largely reduce the pathogenicity and conidium generation ability after the cabbage wither disease bacteria is inactivated.

Description

Technical field [0001] The present invention relates to genes which are derived from Fusarium wilt of Brassica oleracea and affect pathogenicity and conidia production and their applications in the field of plant protection. Background technique [0002] Fusarium oxysporum f.sp.conglutinans is a worldwide fungal soil-borne disease caused by Fusarium oxysporum (Hao Xiaojuan, Liu Bo, Xie Guanlin. Research progress in biological control of plant fusarium wilt[J].Chinese Agricultural Science Bulletin , 2005, 21(7): 319-322.), Fusarium oxysporum belongs to Fungiimperfecti, Moniliales, Tubercular, and Fusarium. It can cause fusarium wilt of more than 100 kinds of plants such as melons, solanaceae, legumes, cruciferous plants, flowers and cash crops (Harter L L. Fusarium wilt of cabbage[J].Science,1909,30(782): 934. Li Mingyuan, Zhang Taotao, Li Xinghong, et al. Cruciferous vegetable fusarium wilt and its pathogen identification[J]. Plant Protection, 2003,29(3):44-45.). The planting a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/37C12N15/31C12N15/63C12N1/15C12N1/14C12R1/77
Inventor 燕继晔李兴红张玮周莹刘梅黄金宝冯建海
Owner 京科智宝(北京)农业科技有限公司
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