Live pseudorabies vaccine and preparation method thereof

A pseudorabies and vaccine technology, applied in the field of vaccine preparation, can solve the problems of vaccine efficacy decline, epidemic, immune failure disease, etc., achieve high aging resistance, reduce damage, solve energy waste and inconvenient storage effects

Active Publication Date: 2015-08-19
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of vaccine production, transportation and use, if the cold chain system in any link is not properly controlled, the high temperature environment will lead to a decrease in the efficacy of the vaccine, and eventually cause the epidemic due to immunization failure

Method used

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  • Live pseudorabies vaccine and preparation method thereof
  • Live pseudorabies vaccine and preparation method thereof
  • Live pseudorabies vaccine and preparation method thereof

Examples

Experimental program
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Embodiment Construction

[0013] In the field of vaccine lyoprotectant preparation, the selection and ratio of the protective agent components have an important impact on the preservation effect of the vaccine. The applicant has conducted long-term research on the components of the lyoprotectant of the present invention, and optimized the proportioning concentrations among each other, so that each component has the greatest compounding effect.

[0014] The components used for the lyoprotectant of the present invention are described as follows:

[0015] 1. NZ-Amine AS, also known as the enzymatic hydrolyzate of casein;

[0016] 2. Monopotassium glutamic acid: it is a surfactant that can reduce the tension of the interface. Acts as a wetting agent for the active ingredients.

[0017] 3. Sucrose has osmotic effect, can inhibit the growth of harmful microorganisms, prolong the storage period of products, and has good water solubility. It can improve the survival rate of microorganisms, form a uniform su...

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Abstract

The invention relates to a freeze-drying protective additive which is composed of 1 to 2% of NZ-Amine AS, 0.1 to 0.2% of monosodium glutamate, 5 to 10% of saccharose, 1 to 2% of M199, 0.1 to 0.3% of trehalose, 8 to 15% of hydrolyzed gelatin, and the balance of water. The freeze-drying protective additive is used for preparing a freeze-dried pseudorabies vaccine. A heat-resisting protective agent can minimize the damage of various physical and chemical factors of a product to virus activity during sub-packaging and freezing-drying processes, greatly reduces the titer loss of the virus before and after freezing and drying and keeps the loss of being not greater than 0.2 titer. A heat-resisting protective agent live vaccine is high in degree of resistance to aging, the loss ratio of the virus kept for 10 days under a condition of 37 DEG C is not greater than 0.5 titer; the heat-resisting protective agent live vaccine can be stored for 24 months at 2 to 8 DEG C, the titer of the vaccine is reduced a little, so that the problem that the vaccine in general transporting and storing needs strict cold chain condition, which leads to energy waste and inconvenient storage, can be solved effectively.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, in particular to a pseudorabies live vaccine and a preparation method thereof. Background technique [0002] Freeze-dried live vaccines are one of the main vaccines for preventing livestock and poultry infectious diseases, but the freeze-dried protective agents commonly used by domestic vaccine companies are simple in composition and poor in protection performance; if stored at 2-8°C, the shelf life is only short Most of them need to be stored at -15°C for 3 to 6 months. This requires that the vaccine be stored, transported and used as low temperature as possible, otherwise the potency of the vaccine will be greatly reduced. In the process of vaccine production, transportation and use, if the cold chain system in any link is not properly controlled, the high temperature environment will lead to a decrease in the effectiveness of the vaccine, and eventually cause the epidemic due to i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K9/19A61K39/245A61P31/22A61K31/43A61K31/7036
Inventor 管清江邹桂荣刘慧芬吕宁宁郭长文
Owner YEBIO BIOENG OF QINGDAO
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