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Infectious bursal disease live vaccine and production method thereof

A chicken infectious and bursal disease technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of antigenic variation of strains in the field, economic loss, immune failure, etc.

Active Publication Date: 2013-09-25
北京中海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to antigenic variation of strains in the field, super-virulent strains and mutant strains continue to appear, and existing commercial vaccines often fail to immunize, resulting in huge economic losses

Method used

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  • Infectious bursal disease live vaccine and production method thereof
  • Infectious bursal disease live vaccine and production method thereof
  • Infectious bursal disease live vaccine and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] ——— Vaccine Manufacturing

[0083] 1. Materials

[0084] (1) Virus content of chicken infectious bursal disease virus B87 strain 10 6.38 ELD 50 / 0.2ml, provided by Jilin Zhengye Biological Products Co., Ltd.

[0085] (2) Virus content of chicken infectious bursal disease virus CA-C strain 10 6.5 ELD 50 / 0.2ml, provided by Beijing Haidian Zhonghai Animal Health Technology Company.

[0086] (3) Virus content of chicken infectious bursal disease virus CF-C strain 10 6.5 ELD 50 / 0.2ml, provided by Beijing Haidian Zhonghai Animal Health Technology Co., Ltd.

[0087] (4) The virulent chicken infectious bursal disease BC6-85 virulent strain used for inspection was provided by the China Veterinary Drug Control Institute.

[0088] (5) SPF chicken embryos were purchased from Guangdong Dahuanong and Beijing Lanfeng Poultry Breeding Co., Ltd.

[0089] 2. Seed poison preparation for production

[0090] (1) Chicken infectious bursal disease virus B87 strain

[0091] 1) Pr...

Embodiment 2

[0129] ——— Finished product inspection (the vaccine prepared by the embodiment, the batch number is 01 batches)

[0130] 1. Properties The vaccine is a light red spongy loose mass, which is easy to detach from the bottle wall and dissolves quickly after adding diluent.

[0131] 2. The sterility test of the vaccine is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and there is no growth of bacteria and mold.

[0132] 3. The mycoplasma test vaccine was carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and there was no mycoplasma growth.

[0133] 4. Exogenous virus inspection The vaccine is tested according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and there is no exogenous virus contamination.

[0134] 5. Differential test Dilute the vaccine to 1 part / 0.1ml, mix it with the same amount of specific anti-infectious bursal virus serum, neutralize at 37°C for 60 minutes, a...

Embodiment 3

[0144] ----Clinical Trials

[0145] The present inventor has carried out clinical trials with 3 batches of pilot-scale products of chicken infectious bursal disease trivalent live vaccine (B87 strain + CA-C strain + CF-C strain). More than 16,000 Nongda No. 3 laying hens and 18,000 Ross 308 broiler chickens were immunized in the experiment. After the test vaccine was inoculated with layer hens and broiler chickens at the recommended dose and overdose (10 times the recommended dose), no adverse reactions caused by the vaccine were observed. 14 days after overdose inoculation, randomly select laying hens and broilers for necropsy, observe the bursa, liver, kidney, spleen, glandular stomach, gizzard, chest, leg and wing muscles, except for the mild atrophy of the bursa , other organs and muscles had no visible pathological changes caused by chicken infectious bursal disease. 14 days after immunization of broiler and laying hens with dose and 1 / 2 dose, challenge with virulent B...

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Abstract

The present invention relates to an infectious bursal disease live vaccine production method, which screens virulent strains with good immunogenicity, wider antigen spectrum and immune synergy from studies of characteristics of different infectious bursal disease virus such as immunogenicity, antigen spectrum, pathogenicity of bursa of Fabricius and capacity of breaking through maternal antibody, carries out development of the infectious bursal disease trivalent live vaccine, and develops a live vaccine--an infectious bursal disease trivalent live vaccine which has good immunogenicity, can break through higher level maternal antibody, has long immune duration more than 3 months, and has good protection to the various virulent strains.

Description

technical field [0001] The invention relates to a chicken infectious bursal disease live vaccine and a production method thereof. It belongs to the field of veterinary biological products. Background technique [0002] Chicken infectious bursal disease (Infectious bursal disease, IBD) is an acute, contagious disease of chicks caused by chicken infectious bursal virus (Infectious bursal disease virus, IBDV). Cyst edema, hemorrhage, necrosis, atrophy, etc. are characterized by high morbidity, and the mortality rate is generally 5% to 20%, sometimes even as high as 50%. It is one of the most important diseases in the poultry industry. Vaccination is one of the main measures to prevent and control the disease, although there are many commercial vaccines, which play an important role in the prevention and control of the disease. However, due to antigenic variation of strains in the field, super-virulent strains and mutant strains continue to appear, routine vaccine immunization...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61P31/14C12N7/00C12R1/93
Inventor 蒋桃珍陈光华孙晔
Owner 北京中海生物科技有限公司
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