Grifola frondosus solid state fermentation functional beverage and its preparation method
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A technology of solid-state fermentation and grifola frondosa, which is applied in food preparation, application, food science, etc., can solve problems such as few and unreported research, and achieve the effect of enriching nutritional value
Active Publication Date: 2013-09-25
HEBEI UNIVERSITY
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[0005] At present, most of the beverage development of Grifola frondosa is devoted to liquid fermentation. There are few reports on the preparation of functional drinks from
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[0035] The preparation method of the solid-state fermented functional drink of Grifola frondosa comprises the following steps:
[0036] A, use the shake flask culture medium that contains shiitake mushroom or shiitake mushroom stipe and apricot leaf, make mycosphere through shaking flask culture, make protoplast after enzymatic hydrolysis;
[0037] A-1 After activating the purchased Grifola frondosa strains, carry out shake flask culture, make the protoplasts after enzymatic hydrolysis of the obtained bacterium balls, and regenerate them after ultraviolet mutagenesis treatment,
[0038] The shake flask culture medium composition that adopts is: Glucose 20g, potato 120g, bran 50g, shiitake mushroom or mushroom stalk 22g, apricot leaf 8g, add distilled water and dilute to 1000mL;
[0039] Potatoes and wheat bran are boiled for 20 minutes, filtered, and the filtrate is obtained; shiitake mushrooms or shiitake stalks, and apricot leaves are dried, crushed, and sieved through an 80...
Embodiment 2
[0095] In the embodiment of the present invention, the solid-state fermentation compound of Grifola frondosa-lentinus edodes-hawthorn leaves, and then compatible components of mountain apricot, Codonopsis pilosula, wolfberry, Panax notoginseng, Luo Han Guo, the ratio is 5:1:1:1:0.1:0.1 ( w / w); change Panax notoginseng to kudzu root, the same as the rest.
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Abstract
The invention relates to a grifola frondosus functional beverage which comprises the following solid extract products: 5 parts of a solid state fermentation compound of grifola frondosusmushroom or lentinus edodes stem-ansu apricot leaf, 1 part of ansu apricot, 1 part of Radix Codonopsis, 1 part of matrimony vine, 0.1 part of pseudo-ginseng and 0.1 part of grosvenor momordica fruit. An extraction method comprises the following steps: extracting by the use of distilled water or softened water, carrying out leaching at 45-55 DEG C and at the pressure of 0.06 MPa according to the solid-liquid ratio of 1:10 for 20 min, and filtering to obtain a first leaching liquor; adding water of the first extraction water volume into filter residue, carrying out digestion under the above same condition for 20 min, and filtering to obtain a second leaching liquor; and mixing the two leaching liquors to obtain an extract. By the cellulose decomposition capability of grifola frondosus, solid fermentation of grifola frondosus is performed, thus being beneficial to release of mushroom or lentinus edodes stem polysaccharide, amino acid and ansu apricot leaf general flavone substance, dietary fiber and carbohydrate and beneficial to synthesis of grifola frondosus metabolite and enriching of nutritive value of grifola frondosus. Therefore, composition of a fermentation product system is more diversified.
Description
technical field [0001] The present invention relates to a method of using shiitake mushrooms or shiitake stalks and apricot leaves as special substrates, and through cell engineering, a method for directional selection of excellent strains of the rare edible and medicinal fungi of Grifola frondosa, and using shiitake mushrooms or shiitake stalks and apricot leaves as the main A method for preparing a functional drink through solid fermentation of Grifola frondosa under a special matrix composed of ingredients. Background technique [0002] Grifola frondosa (Grifola frondosa), also known as Bayleaf Polypore, Chestnut Mushroom, Cloud Mushroom, Lotus Fungus, etc., belongs to Basidiomycetes, Polyporaceae, Polyporaceae, and Polyporaceae. The fruiting bodies of Grifola frondosa are soft, not only rich in high-quality protein, vitamins and trace elements, but can be used as delicious food; at the same time, Grifola frondosa is also a very precious medicinal fungus. A large number o...
Claims
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