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Method for preparing compound enzyme and probiotic preparation for feed through biotransformation of sorghum straws and rapeseed meals

A technology of rapeseed cake and probiotic preparation, applied in animal feed, animal feed, application, etc., can solve the problems of difficult digestion of monogastric animals, and achieve the effect of easy industrialized large-scale production, easy operation, and extensive fermentation process

Inactive Publication Date: 2013-09-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Mix 10kg of ripe Pu'er tea, 5kg of mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of sorghum stalks, 5kg of rapeseed meal, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water. After 48 hours of acclimatization, the starter is formed;

[0028] 2) Mix 20kg of starter in step 1) with 600kg of sorghum straw, 300kg of rapeseed meal, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a thickness of 15cm, natural ventilation, at 40℃ After 4 days of fermentation, a compound enzyme and probiotic preparation for feed is obtained.

[0029] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed of this example are: carboxymethyl cellulase activity reaches 77.23IU / g; total cellulase activity reaches 6.88IU / g; beta-glucosidase activity Reach 15.56IU / g; Endo-glucanase activity reaches 55.90IU / g; Xylanase activity reaches 58.57IU / g; Beta-xylosidase activity reaches 5.64IU / ...

Embodiment 2

[0031] 1) Mix 10kg of ripe Pu'er tea, 5kg of mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of sorghum stalks, 5kg of rapeseed meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization, the starter is formed;

[0032] 2) Mix 20kg of starter in step 1) with 600kg of sorghum straw, 300kg of rapeseed meal, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a thickness of 15cm, natural ventilation, at 40℃ After 4 days of fermentation, a compound enzyme and probiotic preparation for feed is obtained.

[0033] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed of this example are: carboxymethyl cellulase activity reaches 76.78IU / g; total cellulase activity reaches 6.96IU / g; beta-glucosidase activity Reaching 15.42IU / g; endoglucanase activity reaching 55.46IU / g; xylanase activity reaching 58.35IU / g; beta-xylosidase activity reaching 5....

Embodiment 3

[0035] 1) Mix 10kg of ripe Pu'er tea, 5kg of mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of sorghum stalks, 5kg of rapeseed meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization, the starter is formed;

[0036] 2) Mix 10kg of starter in step 1) with 600kg of sorghum straw, 300kg of rapeseed meal, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water. Use solid-state fermentation with a thickness of 10cm, natural ventilation, and fermentation at 35°C. In 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed of this example are: carboxymethyl cellulase activity reaches 74.92IU / g; total cellulase activity reaches 6.14IU / g; beta-glucosidase activity Reaching 15.51IU / g; endoglucanase activity reaching 52.35IU / g; xylanase activity reaching 59.66IU / g; beta-xylosidase activity reaching 5.88...

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Abstract

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed through the biotransformation of sorghum straws and rapeseed meals. The method comprises the steps of: thoroughly mixing cooked Pu'er tea, mushroom sticks, tea tree mushroom sticks, first sorghum straws, first rapeseed meals, first wheat bran, first apple pomace and first water which are subjected to pile fermentation, and carrying out acclimation culture so as to form a leavening agent; and thoroughly mixing the leavening agent, second sorghum straws, second rapeseed meals, second wheat bran, second apple pomace and second water, and carrying out fermentation, thereby obtaining the compound enzyme and probiotic preparation for feed. According to the method, the compound enzyme and probiotic preparation with better various performance indexes is prepared through fermenting the sorghum straws and the rapeseed meals by using food-grade safe flora with stable microbial states, so that the low-cost and high-efficiency biotransformation of the sorghum straws and the rapeseed meals is realized. Meanwhile, the method disclosed by the invention has the advantages that microbes grow easily, the enzyme activity is high, the fermentation process is extensive, and the industrial large-scale production is facilitated.

Description

Technical field [0001] The invention belongs to the field of microbial fermentation, and specifically relates to a method for fermenting sorghum stalks and rapeseed cake by using microecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed ingredients, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulose by-products. There are many types of lignocellulose, such as various crop straws, municipal fiber wastes, agricultural by-products such as corn cobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose, lignin and Pectin, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖徐娟李青青于骅陈功诸文颖杨璐杜姗姗吴渊纪晓燚
Owner ZHEJIANG UNIV
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