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Method for preparing cordyceps preparations with high adenosine contents

A technology with high adenosine content, applied in the field of bioengineering, can solve the problems of lack of physiological activity and pharmacological function, low content of adenosine, etc., and achieve the effects of improving nutritional conditions, increasing the content of adenosine, and promoting growth and reproduction

Inactive Publication Date: 2013-09-04
台建祥 +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, in the Cordyceps sinensis preparations sold in the market, the adenosine content is relatively low. For example, a Cordyceps capsule product currently being advertised on CCTV and Beijing Evening News contains only 4.4mg of adenosine in 100g, which is actually such a low adenosine content. Cordyceps preparations with glucoside content do not have the physiological activity and pharmacological functions mentioned in the propaganda at all

Method used

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  • Method for preparing cordyceps preparations with high adenosine contents
  • Method for preparing cordyceps preparations with high adenosine contents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Cultivation of solid strains of Cordyceps sinensis slope

[0044] Transplant the Mortierella sinensis strain on the PDA slant medium, cultivate and activate it to obtain the mother species of the slant. By weight percentage, the culture medium contains 18% potato, 2.5% glucose, and 2.8% agar, and the culture condition: 20 ℃, pH 5.5, culture time 9 days.

[0045] (2) Shaker seed culture

[0046] Shaking table seed culture condition is that after 9 days of culture on the solid slant of the strain, the mycelium is picked and inoculated in a shaker flask equipped with a liquid medium for cultivation. The main components of the liquid medium are 100 grams of glucose per 1000 milliliters, yeast powder 60 g, 80 ml of peptone hydrolyzate (v / v), 0.5 g of M g SO 4 ·7H 2 O, 0.5 g KH 2 PO 4 , the pH value is 5.5; the temperature is 21° C., the rotational speed is 200 rpm, and the time is 3 days.

[0047] (3) Liquid submerged fermentation culture

[0048] After 3 days of...

Embodiment 2

[0061] (1) Cultivation of solid strains of Cordyceps sinensis slope

[0062] The Paecilomyces hematalis strain is transferred on the PDA slant medium, cultivated, activated, and the slant mother species is obtained. In the medium, by weight percentage, it contains 20% potato, 2.8% glucose, and 2.9% agar. Culture conditions: the temperature is 22°C, the pH is 5.6, and the culture time is 10 days.

[0063] (2) Shaker seed culture

[0064] After 10 days of culture on the solid slant of the strain, pick the mycelium and inoculate it in a shaker flask equipped with a liquid medium for culture. The main components of the liquid medium are 90 grams of glucose per 1000 ml, 70 grams of yeast powder, and peptone hydrolyzate 90ml (v / v), 0.5g MgSO 4 ·7H 2 O, 0.5 g KH 2 PO 4 , the pH value is 5.6; the temperature is 22° C., the rotational speed is 210 rpm, and the time is 4 days.

[0065] (3) Liquid submerged fermentation culture

[0066] After 4 days of seed culture in a liquid sha...

Embodiment 3

[0078] (1) Cultivation of solid strains of Cordyceps sinensis slope

[0079] Inoculate the strains of the genus Eustoma on the PDA slant medium, cultivate and activate it to obtain the slant mother clock, and the medium contains 19% potato, 3% glucose, and 3% agar. Culture conditions: the temperature is 23° C., the pH value is 5.8, and the culture time is 9 days.

[0080] (2) Shaker seed culture

[0081] After 8 days of culture on the solid slant of the strain, the mycelium was picked and inoculated in a shaker flask equipped with a liquid medium for cultivation. The main components of the liquid medium were 60 grams of glucose per 1000 ml, 80 grams of yeast powder, and 30 grams of peptone hydrolyzate. ml, 1.0 g MgSO 4 ·7H 2 O, 0.2 g KH 2 PO 4 , the pH value is 5.7; the temperature is 23° C., the rotational speed is 220 rpm, and the time is 5 days.

[0082] (3) Liquid submerged fermentation culture

[0083] After 5 days of seed culture in a liquid shaker, inoculate into...

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Abstract

The invention relates to a method for preparing cordyceps preparations with high adenosine contents. The method comprises the following steps of: (1), carrying out solid culture and shake culture on fungi strains separated from cordyceps sporocarp to obtain mycelium; (2) inoculating the mycelium obtained in the step (1) in a culture liquid containing a cassava silkworm chrysalis water extract for deep fermentation to obtain deeply fermented mycelium; (3) treating the deeply fermented mycelium obtained in the step (2) at super-high pressure, so that the walls of the mycelium and microorganism are instantly broken, centrifuging fermentation liquor and separating to obtain the mycelium and a bacterium liquid; and (4) finely filtering the bacterium liquid obtained in the step (3), concentrating at the low temperature to prepare cordyceps oral liquid containing high adenosine content, drying the mycelium at the low temperature, carrying out super-fine grinding, so as to prepare nano cordyceps micropowder containing the high adenosine content. The adenosine contents of the cordyceps liquid preparation and the cordyceps solid preparation which are obtained by the method are obviously improved compared with the adenosine contents in the prior art.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a preparation method of a cordyceps preparation. Background technique [0002] So far, people have detected or isolated a variety of biologically active substances from Cordyceps sinensis (Berk.) Sacc.) and its anamorphs, such as fungal polysaccharides, sterols, alkaloids, organic acids, vitamins, amino acids, mannitol Wait. These active ingredients are closely related to the pharmacological functions of Cordyceps sinensis. [0003] Adenosine is 3'-deoxyadenosine (3'-Deoxyadenosine), also known as cordycepin or cordycepin, it is the first nucleoside antibiotic isolated from fungi. Its molecular formula is C 10 h 13 N 5 o 3 , the molecular weight is 251Da, the melting point is 230-231°C, soluble in water, hot ethanol and methanol, insoluble in benzene, ether, chloroform, anthrone reaction is positive, and the maximum absorption wavelength of ultraviolet light is 259nm. Its stru...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61K35/64
Inventor 台建祥何建刘晓伟
Owner 台建祥
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