Photon encoding microsphere-based multielement biomarker assay kit-type chip

Abstract

The invention discloses a photon encoding microsphere-based multielement biomarker assay kit-type chip. The photon encoding microsphere-based multielement biomarker assay kit-type chip comprises a chip main body at an upper part and a substrate at a lower part. The interior of the chip main body is etched with multiple independent detection units. Each one of the detection units comprises an exhaust vent, a reaction cabin, a channel and a sampling hole. The sampling hole is communicated with the reaction cabin by the channel. The exhaust vent communicated with environment outside the chip main body is arranged in the reaction cabin. Microspheres as detection carriers are enclosed in the reaction cabin and biological molecular probes are fixed on surfaces of the microspheres. According to the invention, a photonic crystal liquid phase chip technology as a base and a self-developed PDMS kit-type chip are combined so that operation difficulty of a photonic crystal liquid phase chip analysis technology is effectively reduced and an operation process is standardized and later-stage automatic detection development is promoted. The detection method adopting the photon encoding microsphere-based multielement biomarker assay kit-type chip is simple and efficient and can replace the existing clinical analysis technology.

Description

technical field [0001] The invention relates to a multi-component biomarker detection cassette chip based on photon-coded microspheres and a preparation method thereof. It can be used to detect biological macromolecules such as proteins and nucleic acids, and can be widely used in clinical testing, inspection and quarantine, environmental monitoring, drug screening, microbial identification, and functional analysis of nucleic acids and proteins. Background technique [0002] Multiplex detection technology has attracted extensive attention due to its high-throughput and low-time-consuming methodological characteristics. Its initial form is a gene chip, which is mainly used in genomics research. Until the end of the last century, the emergence of liquid-phase chip technology made multiple detection technologies emerge in proteomics research and clinical practical applications, and were mostly used in the screening of new markers, the analysis of large samples, and the typing ...

AI Technical Summary

Problems solved by technology

In the multiple detection repeated experiments based on photonic crystal-encoded microspheres, the difficulty of handling the microspheres has become an obstacle that limits its further application

Because the size of the microspheres is too small, it is difficult to distinguish with the naked eye, so in the non-chip detection mode, the microspheres are often damaged artificially, or the microspheres are lost

Since each experimental data obtained is the average of the data measured by multiple microspheres, the damage or loss of the microspheres will greatly reduce the accuracy and repeatability of the experimental data, resulting in a reduction in the accuracy of the method

At the same time, the uncontrollable experimental error between different operators brought about by the non-chip detection mode further affects the accuracy and repeatability of the method

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