Microalgae activity detection method and device in ship ballast water

Abstract

The invention relates to a microalgae activity detection method and a device in ship ballast water. The device comprises a platform, a micro fluid chip, a driving assembly, an optical excitation assembly, an optical detection assembly and a data processing assembly, wherein the platform is of a darkroom structure; and the optical detection assembly is connected with the platform, the micro fluid chip and the optical detection assembly are fixed in the platform, the driving assembly is connected with the micro fluid chip, and the optical detection assembly is connected with the data processing assembly. The microalgae activity detection method and the device provided by the invention have the beneficial effects that the structure is simple, the carry is convenient, and the detection method is simple and scientific.

Description

technical field [0001] The invention relates to a method and a device for detecting microalgae activity in ship ballast water. Background technique [0002] The ship's ballast water is one of the main ways for the invasion of alien marine organisms. The disasters caused by the invasion and spread of harmful alien marine organisms have caused serious impacts on ecology, industry, agriculture, and human health. In "Ship Ballast Water and Sedimentation The International Convention on the Control and Management of Substances stipulates the standards that the ballast water performance of ships should meet when they are discharged. One of the important contents is the concentration of living organisms of different sizes in the ballast water, and microalgae are the most common in the ballast water of ships. A living organism that exists and is currently the main target for ship ballast water treatment and testing. [0003] At present, the methods for ship ballast water detection m...

AI Technical Summary

Problems solved by technology

This method overcomes the shortcomings of artificially observing the activity of microalgae, and improves the accuracy to a certain extent; however, each dye can only be used for some algae according to its own dyeing mechanism, and the universality is poor, and the dyeing process is cumbersome, requiring Personnel with professional knowledge carry out dyeing, the dyeing process is easily disturbed by various external factors, and some dyes themselves are toxic

[0006] Flow cytometer counting method, that is to use commercial flow cytometer to count microalgae cells. The suspension made of microalgae cells labeled with fluorescent dyes passes through the detection area at a certain flow rate. Under the action of excitation light, specific fluorescent signals and scattered light signals are emitted, and the size, quantity, and internal substances of microalgae cells are judged according to these signals, which overcomes counting errors caused by manual operations, and has the advantages of high work efficiency and fast accuracy. , is one of the standard methods for the determination of biomass at present; the disadvantages of this method are: the commercial flow cytometer is expensive, bulky, inconvenient for on-site detection, cumbersome pre-treatment of samples, and complicated operation. In addition, the judgment of algae activity still relies on Fluorescent dyes, the above-mentioned shortcomings of fluorescent dyes have not been resolved

[0007] Molecular and biochemical methods mainly include: nucleic acid hybridization technology, polymerase chain reaction (PCR) technology, gene chip technology, DNA fingerprint technology, enzyme activity analysis, etc. The advantages are: high detection sensitivity, which is the technology with the highest detection sensitivity at present; disadvantages Yes: There is nothing that can be done for the volume measurement of microalgae, the required equipment is expensive and cumbersome, and it is basically used to collect samples and take them back to the laboratory for culture identification, which is time-consuming and laborious, and the efficiency is not high, and the types of detection are relatively limited. New species lack understanding to confirm, and the method requires highly trained technicians to prepare samples and analyze results

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