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Economic and efficient spirulina platensis mixed culture method

A high-efficiency technology for Spirulina platensis, applied in the field of polyculture of Spirulina platensis, can solve the problems of cost and effective utilization rate bottleneck, unfavorable growth and fixation of microalgae, low availability of microalgae, etc., and achieve fast carbon fixation Efficiency, waste reduction, and simple equipment

Active Publication Date: 2013-07-24
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

The photoautotrophic process is strictly limited by light conditions: when the light is insufficient or too high, it is not conducive to the growth of microalgae and the 2 The fixation of microalgae; the self-shading phenomenon of microalgae will lead to low availability of light for microalgae in the later stage of culture, thereby seriously inhibiting its photosynthetic growth
The vast majority of microalgae are photoautotrophic microorganisms, but studies have shown that some microalgae can use organic carbon as a carbon source for heterotrophic growth, and the growth process is closely related to the type and concentration of organic carbon, while the cost and effective use of organic carbon Rate is the bottleneck in microalgae cultivation process

Method used

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  • Economic and efficient spirulina platensis mixed culture method
  • Economic and efficient spirulina platensis mixed culture method
  • Economic and efficient spirulina platensis mixed culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 cultivates Spirulina platensis with photoautotrophy, heterotrophy and mixed culture respectively

[0015] The Spirulina platensis cultivated to the logarithmic phase was selected under batch culture conditions, and the biomass, chlorophyll a content, organic carbon (TOC) and Inorganic carbon (IC) content.

[0016] Photoautotrophic conditions: Under photoautotrophic conditions, the light intensity is 3000 Lux, the photoperiod is 16 / 8, and CO with a concentration of 10% is introduced in the form of bubbling during the light time 2 , the gas velocity is 0.2vvm, the aeration time is 30min / d, and the medium is Zarrouk medium.

[0017] Heterotrophic conditions: Add glucose at an initial concentration of 1 g / L to Zarrouk medium without light.

[0018] Mixed culture conditions: add glucose with an initial concentration of 1g / L to Zarrouk medium, and the light and aeration conditions are the same as the photoautotrophic conditions.

[0019] As shown in Table 1, u...

Embodiment 2

[0025] The influence of different carbon sources on the growth of Spirulina platensis during embodiment 2 polyculture

[0026] Glycerol, glycine, lactic acid, sodium acetate, fructose and glucose were added to the Zarrouk medium respectively, and the concentration range was 1-5g / L. like figure 1 As shown, acetate, fructose and glucose can be used as organic carbon sources in the mixed culture of Spirulina platensis, which can significantly promote the growth of algae cells; in addition, glycine and lactose can also promote the growth of cells to a certain extent. The effect of glucose and fructose is better than that of lactose, probably because the former two are monosaccharides, and lactose is a disaccharide, and the hydrolysis and assimilation of the latter requires a certain amount of time and energy; the effect of glucose is better than that of acetate, which may be due to Glucose yields more energy than sodium acetate (2.8 kJ / mol for glucose versus 0.8 kJ / mol for sodium...

Embodiment 3

[0027] Embodiment 3 Optimizing the light intensity in the polyculture growth process of Spirulina platensis

[0028] On the basis of ensuring the biomass, optimize the light intensity during the mixed growth of Spirulina platensis. During the whole mixed culture process of this experiment, the growth curve of Spirulina platensis can be divided into four stages: (1) The first stage (P1), 0-24h; (2) the second stage (P2), 24-48h; (3) the third stage (P3), 48-64h; (4) the fourth stage (P4), 64- 96h.

[0029] like figure 2 , image 3 As shown, in the first stage, when the concentration of algae cells is low, the algal biomass under the treatment of 2000, 3000, 4000, 5000 and 6000Lux is equivalent, which is higher than that under the treatment of 1000Lux, which is 0.7g / l. This can be explained as, Under the low algae density, the light intensity is too low to satisfy the growth of algae under the treatment of 1000Lux, and the light intensity under the treatment of 6000Lux leads...

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Abstract

The invention provides an economic and efficient spirulina platensis mixed culture method, belonging to the technical field of microalga biology. During the growth process of spirulina platensis, the illumination intensity is controlled in stages, and glucose is fed as a heterotrophic carbon source, so that the technical problems that the spirulina platensis is limited by light intensity, concentration of an organic carbon source and the like during the culture process are solved, an optimal feeding way with most appropriate light intensity and carbon source in the mixed culture process is found, and high-efficient carbon fixation and fast growth of the spirulina platensis are realized.

Description

technical field [0001] The present invention relates to a kind of polyculture method of economical and efficient Spirulina platensis, and more specifically studies the influence of three culture modes (photoautotrophy, polyculture, heterotrophy) and light intensity on the growth and carbon fixation of Spirulina , belongs to the field of microalgae biotechnology. Background technique [0002] The growth of microalgae is mainly divided into photoautotrophic, heterotrophic and mixed culture according to the nutritional mode. The photoautotrophic process is strictly limited by light conditions: when the light is insufficient or too high, it is not conducive to the growth of microalgae and the 2 The self-shading phenomenon of microalgae will lead to low availability of light for microalgae in the later stage of culture, thereby seriously inhibiting its photosynthetic growth. The vast majority of microalgae are photoautotrophic microorganisms, but studies have shown that some mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 阮文权任洪艳李姿杨玉娟
Owner JIANGNAN UNIV
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