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Method for extracting high purity 1-deoxynojirimycin

A deoxynojirimycin and extraction method technology, which is applied in the field of extraction of high-purity 1-deoxynojirimycin, can solve the problems of low extraction rate and purity of the product, and high toxicity of the product, so as to improve the extraction rate and product quality and safety , the effect of high application value

Active Publication Date: 2013-07-17
CHENGDU KEYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] The purpose of the present invention is to overcome the shortcomings of low product extraction rate and purity and high product toxicity in the prior art, and provide a high-purity 1-deoxynojirimycin extraction method with high extraction rate, product safety, and environmental protection.

Method used

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  • Method for extracting high purity 1-deoxynojirimycin

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Effect test

Embodiment 1

[0048] A method for extracting high-purity 1-deoxynojirimycin, comprising the steps of:

[0049] 1. Raw material pretreatment: Take the mulberry branches, dry them, crush them, pass through a 40-mesh sieve, and obtain the raw material powder for extraction;

[0050] 2. Enzymatic extraction:

[0051] (1) Add 8 times the weight of water to the raw material powder, heat up to 47±2°C, add 0.5% of the combined enzyme of the raw material powder weight, keep warm, enzymolysis and leaching for 8 hours, and then obtain the filter residue and filtrate through centrifugal filtration; The composition and weight ratio of the combined enzymes are cellulase: dextranase: pectinase=3: 2: 1;

[0052] (2) Repeat enzymatic hydrolysis and leaching of the filter residue, the operation is the same as step (1), and filter;

[0053] (3) merging step (1) and step (2) twice leaching filtrate;

[0054] 3. The leaching filtrate is centrifuged to remove the slag with a centrifuge, and then the pH value ...

Embodiment 2

[0061] A method for extracting high-purity 1-deoxynojirimycin, comprising the steps of:

[0062] 1. Raw material pretreatment: take mulberry leaves, dry them, crush them, pass through a 40-mesh sieve, and obtain the raw material powder for extraction;

[0063] 2. Enzymatic hydrolysis and extraction: add 12 times the weight of water to the raw material powder, after heating up to 58±2°C, add 0.8% of the combined enzyme of the raw material powder weight, keep warm, enzymatic hydrolysis and extraction for 10 hours, and then centrifuge to obtain the leaching Extract the filtrate; the composition and weight ratio of the combined enzymes are cellulase: dextranase: pectinase=5: 4: 1;

[0064] 3. The leaching filtrate is centrifuged to remove the slag with a centrifuge, and then the pH is adjusted to 4-5 with acid;

[0065] 4. Put the acidified filtrate on a strongly acidic cationic resin column, rinse with purified water 4 times the volume of the resin, collect the washing liquid, a...

Embodiment 3

[0070] A method for extracting high-purity 1-deoxynojirimycin, comprising the steps of:

[0071] 1. Raw material pretreatment: Take sericulture waste (silkworm excrement) and grind it after drying, and pass through a 30-mesh sieve to obtain the raw material powder for extraction;

[0072] 2. Enzymatic extraction:

[0073] (1) Add 10 times the weight of water to the raw material powder, heat up to 55±2° C., add 0.5% of the combined enzyme of the raw material powder weight, keep warm, enzymolysis and leaching for 12 hours, and then centrifuge to obtain the leaching filtrate; The composition and weight ratio of the combined enzymes are cellulase: dextranase: pectinase=4: 3: 1;

[0074] (2) Repeat enzymatic hydrolysis and leaching of the filter residue, the operation is the same as step (1), and filter;

[0075] (3) merging step (1) and step (2) twice leaching filtrate;

[0076] 3. The leaching filtrate is centrifuged to remove the slag with a centrifuge, and then the pH is adj...

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Abstract

The invention discloses a method for extracting high purity 1-deoxynojirimycin. The method comprises the following steps: pretreating raw materials; conducting enzymolysis digestion; acidifying a filtrate; filling the filtrate into a strong acidic cationic resin column; eluting with ammonia to obtain an ammonia eluate; conducting membrane separation on the ammonia eluate to remove impurities, and concentrating; conducting column chromatography on the concentrated solution by a weak acidic cationic resin column; eluting by ammonia water; collecting the eluate by segment; and concentrating, crystallizing and drying to obtain a 1-deoxynojirimycin refined product. The enzymolysis digestion step uses a combined enzyme comprising cellulase, glucanase, pectinase in a weight ratio of 3-5:2-4:1. The method for extracting high purity 1-deoxynojirimycin provided by the invention uses an enzymolysis method, which is organically combined with the technologies of impurity removal and purification; therefore, the extraction method provided by the invention has the advantages of high extraction rate and purity higher than 98% of a target product, no environmental pollution and improved product quality and safety.

Description

technical field [0001] The invention relates to a method for extracting natural substances from plants, in particular to a method for extracting high-purity 1-deoxynojirimycin. Background technique [0002] Mulberry leaf is the leaf of Moraceae (Moraceae) Morus L., and is a medicinal and edible plant announced by the Ministry of Health. It is cultivated and produced in most parts of the country, and the southern sericulture production area has the largest output, such as Jiangsu , Guangxi, Zhejiang, Anhui, Sichuan, etc. Mulberry leaves are bitter in taste, sweet, cold in nature, and return to the lung and liver channels. It is recorded in ancient Chinese medicine books that mulberry leaves can treat diabetes. Modern medicine has found that mulberry leaves have the functions of lowering blood pressure, lowering blood sugar, lowering cholesterol, anti-tumor, anti-allergic and diuretic on the basis of studying the chemical components of mulberry leaves and animal experiments....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D211/46
Inventor 汤世江
Owner CHENGDU KEYUAN BIOTECH
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