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Method for producing food material with high GABA function with rice endogenous enzyme activation method

A technology of endogenous enzymes and functional foods, applied in food preparation, lysing enzymes, food science, etc., can solve problems that are still in its infancy, and achieve the effect of improving brain function and liver function

Inactive Publication Date: 2013-07-03
NANJING UNIV OF FINANCE & ECONOMICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2007, Japan's Coca-Cola Company launched the GABA functional drink "AQUARIUS SHARP CHARGE" with relaxation and anti-stress effects for the public. develop and improve

Method used

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  • Method for producing food material with high GABA function with rice endogenous enzyme activation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of GABA (γ-aminobutyric acid): Weigh 200g of brown rice (brown rice is prepared from rice hulling and removing impurities) and place it in a shallow dish. First rinse with tap water to remove impurities and dust on the surface. Add 4 volumes of water and soak for 3 hours at 25°C. Spread the soaked brown rice evenly on a shallow pan spread with four layers of gauze, add an appropriate amount of water, cover with moist gauze, and place it at 25°C to germinate for 24 hours and spray water regularly. Weigh 50g of germinated brown rice, add 200mL of water and grind it into a slurry with a high-speed tissue masher, take a certain amount of brown rice slurry, add 8.0mmol / L substrate L-glutamic acid, 0.2mmol / L coenzyme PLP, 0.2 mmol / L activator CaCl 2 , It is transformed into GABA in PBS buffer with pH 6.1, and the transformation temperature is controlled to 37.5℃.

[0024] (2) GAD (glutamate decarboxylase) extraction: Weigh 100g of germinated brown rice, add 50m...

Embodiment 2

[0027] (1) Preparation of GABA (γ-aminobutyric acid): Weigh 200g of brown rice (brown rice is prepared from rice hulling and removing impurities) and place it in a shallow dish. First rinse with tap water to remove impurities and dust on the surface. Add 4 volumes of water and soak for 6 hours at 30°C. Spread the soaked brown rice evenly on a shallow pan spread with four layers of gauze, add an appropriate amount of water, cover with moist gauze, and place it at 30°C to germinate for 36 hours and spray water regularly. Weigh 50g of germinated brown rice, add 200mL of water and grind it into a slurry with a high-speed tissue masher, take a certain amount of brown rice slurry, add 8.5mmol / L substrate sodium glutamate, coenzyme PLP, 0.2mmol / L activator CaCl 2 , It is transformed into GABA in PBS buffer with pH 6.2, and the transformation temperature is controlled to 38.0℃.

[0028] (2) GAD (glutamate decarboxylase) extraction: Weigh 200g of brown rice, add 800ml extract (50mmol / L ...

Embodiment 3

[0031] (1) Preparation of GABA (γ-aminobutyric acid): Weigh 200g of brown rice (brown rice is prepared from rice hulling and removing impurities) and place it in a shallow pan. First rinse with tap water to remove impurities and dust on the surface. Add 4 volumes of water and soak for 9 hours at 35°C. Spread the soaked brown rice evenly in a shallow pan spread with four layers of gauze, add an appropriate amount of water, cover with moist gauze, and place it at 30°C to germinate for 46 hours and spray water regularly. Weigh 50g of germinated brown rice, add 200mL of water and grind it into a slurry with a high-speed tissue masher, take a certain amount of brown rice slurry, add 9.0mmol / L substrate sodium glutamate, coenzyme PLP, 0.2mmol / L activator CaCl 2 , In pH 6.3 PBS buffer (containing 50mmol / L phosphate buffer (pH5.7), 0.2mmol / L pyridoxal phosphate (PLP), 2mmol / L EDTA, 0.2% β-mercaptoethanol, 0.15mol / L NaCL) is converted to GABA, and the conversion temperature is contro...

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Abstract

The invention discloses a method for producing a food material with a high GABA (gamma amino acid butyric acid) function with a rice endogenous enzyme activation method. A glutamic acid decarboxylase activation technology is adopted on the basis that rice endogenous glutamate decarboxylase characteristics and an activity regulatory mechanism are defined, so that healthy rice food with a high GABA content is produced; germinated brown rice rich in GABA is taken as a raw material, and with the adoption pretreatment of aminotransferase for passivating and decomposing GABA, germ-remained GABA polished rice is produced; a natural nutrient solution containing a glutamic acid decarboxylase activating agent and a special buffer system is utilized for sprouting; and a rice husk and a rice germ are taken as a GAD (glutamic acid decarboxylase) source, a food ingredient rich in GABA is produced after the GAD source is immobilized, and the food ingredient is applied to GABA nutrition coordination rice powder production. The method is simple and convenient, development of food materials rich in GABA functional factors can be accelerated, and the food material can be used as or for functional food which is resistant to hypertension and can promote brain functions and liver functions.

Description

Technical field [0001] The invention relates to the field of food, in particular to a method for producing high-GABA functional food materials by using the endogenous enzyme activation method of rice to improve the structure and nutritional structure of food products. Background technique [0002] As a biologically active factor with multiple physiological functions, GABA has been widely used in nutritional supplements and functional foods in the United States, Japan and other countries. In 1996, GABA-rich rice germ products were commercialized in Japan. Japanese scientists use rice germ and other raw materials to develop and manufacture GABA-rich functional food ingredients and apply them to beverages, jams, cakes, biscuits, seasonings and other products. Existing reports show that high-concentration GABA beverages made with biotechnology and GABA-rich food ingredients made with lactic acid bacteria can be used directly or used as functional foods for anti-hypertension, brain fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/105A23L1/185A23L1/29C12N9/88A23L7/104A23L5/20A23L7/20A23L33/00
Inventor 王立峰陈静宜
Owner NANJING UNIV OF FINANCE & ECONOMICS
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