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Bacillus spp culture medium with coconut water as main raw material

A main raw material, Bacillus technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as environmental pollution and resource waste, and achieve the effect of reducing pollution, increasing added value, and having good application prospects.

Inactive Publication Date: 2013-06-12
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional coconut processing industry in Hainan, coconut water is only used as a waste material and is directly discharged into the surrounding environment, which not only causes serious environmental pollution, but also causes a great waste of resources

Method used

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  • Bacillus spp culture medium with coconut water as main raw material
  • Bacillus spp culture medium with coconut water as main raw material
  • Bacillus spp culture medium with coconut water as main raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0020] Using coconut water as the main raw material for the Bacillus culture medium, the Bacillus subtilis stored at -80°C for 1 year was subjected to a large-scale culture test according to the following steps, as follows:

[0021] 1. Quickly thaw Bacillus subtilis stored at -80°C for 1 year with warm water at 40°C, pipette 1ml of the thawed bacterial suspension into 100ml of stimulating growth medium, and culture at 37°C and 180r / min for 24h;

[0022] 2. Take 1ml of the stimulating growth medium bacterial liquid after 24h of cultivation into 100ml of liquid expansion medium, and cultivate at 37°C and 180r / min for 20h;

[0023] 3. Take 1ml of the liquid expansion culture medium, and dilute it gradually to 10 -6 、10 -7 、10 -8 0.1ml of each of the above-mentioned gradient diluted bacterial solutions was spread on the colony counting medium and counted; 3 parallel groups were set up. The specific results are shown in Table 1.

[0024] Table 1

[0025]

[0026] *, the col...

Embodiment example 2

[0029] The Bacillus culture medium with coconut water as the main raw material, followed the steps below to conduct a large-scale culture test on the common Bacillus subtilis solid products on the market, as follows:

[0030] 1. Aseptic operation Weigh 1g of Bacillus subtilis solid product into 100ml stimulating growth medium, culture at 37°C, 200r / min for 24h;

[0031] 2. Take 1ml of the stimulating growth medium bacterial liquid after 24h of cultivation into 100ml of liquid expansion medium, and cultivate at 37°C and 180r / min for 20h;

[0032] 3. Take 1ml of the liquid expansion culture medium, and dilute it gradually to 10 -6 、10 -7 、10 -8 0.1ml of each of the above-mentioned gradient diluted bacterial solutions was spread on the colony counting medium and counted; 3 parallel groups were set up. The specific results are shown in Table 2.

[0033] Table 2

[0034]

[0035] Result shows, the bacillus substratum that the present invention establishes with coconut wate...

Embodiment example 3

[0037] Using coconut water as the main raw material for the bacillus culture medium, carry out the large-scale culture test on the live bacteria of bacillus licheniformis according to the following steps, specifically as follows:

[0038] 1. Pick a single colony of Bacillus licheniformis purified on LB solid medium ( / L: peptone 10g, yeast powder 5g, agar 15g, pH 7.2, sterilized seawater 1000ml, sterilized at 121°C for 20min) to 100ml to stimulate growth culture medium, cultured at 37°C and 180r / min for 24h;

[0039] 2. Take 1ml of the stimulating growth medium bacterial liquid after 24h of cultivation into 100ml of liquid expansion medium, and cultivate at 37°C and 180r / min for 20h;

[0040] 3. Take 1ml of the liquid expansion culture medium, and dilute it gradually to 10 -6 、10 -7 、10 -8 0.1ml of each of the above-mentioned gradient diluted bacterial solutions was spread on the colony counting medium and counted; 3 parallel groups were set up. The specific results are sh...

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Abstract

The invention discloses a Bacillus spp culture medium with coconut water as a main raw material. The culture medium comprises: (1) a growth stimulation culture medium ( / L); (2) a liquid amplification culture medium ( / L); and (3) a colony counting culture medium ( / L). According to the invention, economical, cheap and widely available coconut water is used as the main raw material for the Bacillus spp culture medium, and a scientific and reasonable application method of the Bacillus spp culture medium is constructed at the same time; with the application method, the culture medium can meet requirements of scale-up culture of Bacillus spp; a leftover of coconut processing in Hainan province, i.e., coconut water, is made full use of, the Bacillus spp culture medium with coconut water as the main raw material is created for the first time, a good cheap culture medium formula is provided for development of scale-up culture of Bacillus spp in the future, and the Bacillus spp culture medium has a good application prospect; an effective approach is provided for high-valued utilization of coconut water, an added value of coconut processing can be obviously increased, and pollution of the industry of coconut processing to the environment can be reduced at the same time.

Description

technical field [0001] The invention relates to a bacillus culture medium with coconut water as the main raw material. Background technique [0002] The extracellular amylase, protease and lipase produced by Bacillus in the process of proliferation can degrade biological residues, residual bait and other organic matter in the aquaculture water into small molecular organic substances, and at the same time, Bacillus can degrade the ammonia nitrogen and nitrous acid in the aquaculture water. Nitrogen, nitrate nitrogen, and COD all have obvious degradation effects, which greatly reduces the aging and eutrophication of aquaculture water, and achieves the purpose of improving and repairing aquaculture water; in addition, Bacillus can also Inhibit the growth of various pathogenic bacteria in aquaculture water. Therefore, microbial preparations developed based on Bacillus have broad application prospects in marine aquaculture systems. [0003] In the large-scale cultivation of Bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/125C12R1/10
Inventor 谢珍玉周永灿吴海武郭伟良
Owner HAINAN UNIVERSITY
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