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Strain for producing mannitol and method for producing mannitol through fermentation of strain

A mannitol and strain technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of low yield, low cost, increase the cost of separation and purification, and achieve the effect of efficient production

Active Publication Date: 2013-06-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is low in cost, but its yield is low, and there are sorbitol by-products, which increase the cost of subsequent separation and purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A mannitol-producing bacterium isolated from sugarcane juice was sent to the China Center for Type Culture Collection to determine its morphological characteristics, physiological and biochemical characteristics, and 16sRNA gene sequence analysis, and was identified as Candida parapsilosis ( Candida parapsilosis ) SK26.003.

Embodiment 2

[0025] Embodiment 2 Fermentation of Candida parapsilosis SK26.003 bacterial strain

[0026] The above strains were cultured on medium A at 30°C for 48 hours. The culture was inoculated in seed medium B containing 100 mL, cultured with ventilation at 30°C for 24 hours, then transferred to 2L fermentation medium C, fermented at 30°C for 3 days, and the stirring speed was 200rpm.

[0027] Medium A: glucose 20g / L, yeast extract 10g / L, agar 2%;

[0028] Seed medium B: glucose 20g / L, yeast extract 10g / L;

[0029] Fermentation medium C: glucose 150g / L, yeast extract 15g / L, urea 1g / L, magnesium sulfate 0.5g / L, sodium chloride 0.1g / L, calcium chloride 0.1g / L, potassium dihydrogen phosphate 2g / L L, adjust the pH to 6.5.

[0030] When the fermentation culture was terminated, the fermentation broth was refrigerated and centrifuged. The concentration of mannitol produced in the supernatant was detected by high performance liquid chromatography (HPLC), and the concentration reached 30 g...

Embodiment 3

[0031] Embodiment 3 Separation and purification of mannitol

[0032] Centrifugal filtration: the fermentation broth is refrigerated and centrifuged to remove bacteria, and the supernatant containing mannitol is collected.

[0033] Decolorization: add 1% activated carbon to the filtrate, keep at 40°C for 40 minutes, filter and separate until the filtrate is clear;

[0034] Desalination: the decolorization solution is desalted through the cation exchange column 001×7 and the anion exchange resin 313;

[0035] Separation and purification: at a separation temperature of 60°C, with calcium-type cation exchange resin as the adsorbent and water as the eluent, mannitol is separated and purified to continuously obtain components rich in mannitol;

[0036] Concentration: Concentrate by rotary evaporation until the solid content is above 70%;

[0037] Crystallization: add 4 times the volume of absolute ethanol to the concentrated solution, and add mannitol seed crystals, keep the tempe...

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Abstract

The invention discloses a strain for producing mannitol and a method for producing mannitol through fermentation of the strain, and belongs to the technical field of food biologics. The invention provides Candida parapsilosis SK26.003 which is screened from sugarcane juice and is preserved in the Chinese Typical Culture Collection Center with the preservation number of CCTCC NO: M2012491. The invention further provides a method for producing mannitol through fermentation of the Candida parapsilosis strain. The method comprises the following steps of: (a) fermenting the strain in a fermentation culture medium containing glucose so as to obtain a fermentation liquid containing mannitol; and (b) separating and purifying mannitol from the fermentation liquid. Mannitol produced by using the strain and the method is safe and reliable, is a functional product with much market potential, and is widely applied to industries such as food, cosmetics, medicament and the like. The strain can be used for producing mannitol in high efficiency and is applicable to large-scale production, and a new method is provided for the industrial preparation of mannitol.

Description

technical field [0001] The present invention relates to a kind of Candida parapsilosis capable of producing mannitol ( Candida parapsilosis ) The screening of SK26.003 and the method for fermenting and producing mannitol by using the yeast strain belong to the field of food biotechnology. Background technique [0002] Mannitol has the characteristics of non-hygroscopic, suitable sweetness, low calorie, non-toxic side effects, metabolism in the human body has nothing to do with insulin, does not increase blood sugar level, and does not cause dental caries. It can be used as a sweetener for diabetics and obese patients. As a food additive, it can also be used for anti-sticking of foods such as maltose, chewing gum, and rice cakes, and as an anti-sticking powder for general cakes. A variety of important fine chemical intermediates can also be synthesized using mannitol as a raw material. [0003] Due to the sharp increase in the consumption of low-calorie food in recent years...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P19/02C12R1/72
Inventor 江波张涛魏文婷沐万孟缪铭
Owner JIANGNAN UNIV
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