Yam seedling in-vitro propagation method

Abstract

The invention discloses a yam seedling in-vitro propagation method comprising the steps of A, taking a yam tender stem with an axillary bud as an explant, and washing with sterile water after the alcohol and mercury bichloride surface is sterilized; B, cutting the stem into stem sections with joints on germ-free paper by using a sterilizing scalpel, and vertically inserting the stem sections into an induction culture medium in a polarity direction; C, culturing at constant temperature under light illumination, and growing cluster buds; D, cutting the cluster buds into a plurality of small cluster buds, and transferring into the induction culture medium for multiplication culture; and E, transferring robust seedlings into a rooting culture medium in a subculture process; opening a bottle and carrying out plantlet hardening when the seedlings are rooted, and then transplanting the seedlings into the medium after washing off; keeping temperature and moisture; and transplanting after the tissue culture seedling survives. The obtained yam seedling has the advantages of good hereditary stability, small propagation occupied space and high propagation speed, so that more than ten thousands of the yamn seedlings can be propagated by one bud for one year. The problem of supplying good-quality yam seedlings in yarn plant can be effectively solved. The established production system is stable, the period is shorter, and the scale quick propagation and production can be satisfied.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a rapid propagation method for yam seedlings, which is a rapid propagation method for Lichuan yam seedlings by using a basic medium and adding different components. Background technique [0002] Hubei Lichuan yam has a planting history of more than 1,500 years in Lichuan City. It is rich in rare trace elements such as selenium and germanium. Vegetables and natural health products. In 2007, Lichuan yam became a geographical indication protection product of the General Administration of Quality Supervision, Inspection and Quarantine; in 2008, the origin of Lichuan yam was recognized as a "national green food raw material standardized production base" by the National Green Food Center; in 2009, Lichuan yam was certified by the National Green Food; in the same year , at the first China Agricultural Products Regional Public Brand Construction Forum held b...

AI Technical Summary

Problems solved by technology

[0003] Lichuan yam generally adopts vegetative propagation technology. Although this technology is simple and easy to implement, most of them use rhizomes as provenance, which reduces the yield to a certain extent, and long-term vegetative propagation leads to its quality degradation, severe pesticide residues, and reduced yield.