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Method for rapidly and concentratedly preparing porphyra haitanensis protoplast

A technology of protoplasts and altar laver, which is applied in botany equipment and methods, plant cells, horticulture, etc., can solve the problems of affecting the adhesion ability, incomplete separation of protoplasts, and the emergence rate of seedlings cannot meet the production requirements, and achieve separation Thorough, low-cost, high-volume results

Inactive Publication Date: 2013-05-22
SHANTOU UNIV
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Problems solved by technology

[0008] At present, Porphyra protoplasts prepared by exogenous enzymatic hydrolysis have been applied for many years, but they have not been promoted in production. The main problem is that the emergence rate cannot meet the production requirements. The reason is probably due to The separation of protoplasts prepared by this method is not complete, which affects its attachment ability

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  • Method for rapidly and concentratedly preparing porphyra haitanensis protoplast
  • Method for rapidly and concentratedly preparing porphyra haitanensis protoplast
  • Method for rapidly and concentratedly preparing porphyra haitanensis protoplast

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Embodiment 1

[0043] The method that the present invention prepares altar laver protoplast comprises the steps:

[0044] 1) Sample selection: Select 30-day-old Porphyra algae (that is, Porphyra fronds) on the cultivation rack. The algae are required to be complete and moderate in size. Wash the surface of the algae with clean seawater to remove epiphytic miscellaneous algae and Inorganic attachments, spare;

[0045] 2) Sample processing and environmental factor regulation: Place the algal bodies cleaned in 1) in a clean culture vessel, and the outside is kept moist by a glass culture vessel one size larger, and the two culture vessels are placed upside down to ensure that the surface of the algae remains In a humid state; put the culture vessel into a room at 20°C, and the light intensity during the day is 8-10μmol / m 2 s, the indoor temperature is automatically controlled by the artificial setting of the heating and cooling air conditioner, the light intensity is provided by the fluorescen...

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Abstract

The invention relates to a method for rapidly and concentratedly preparing porphyra haitanensis protoplast; in the method, porphyra haitanensis frond (thallus) is used as material, through controlling environmental factors such as salt content, light and temperature, the photosynthesis is reduced, the respiration is enhanced and the regular growth is stopped, the vegetative cells of the porphyra haitanensis frond are forced to generate cellulose and pectinase to degrade cell walls, and the cell walls after being degraded give off protoplast with reproductive potential. The method is easy to operate and high in efficiency; by adopting the method, most vegetative cells of the porphyra haitanensis frond can be transformed into dissociative protoplast within 3-5days, the degradation degree of frond cells can reach 60-70%. The method provided by the invention is not to degrade the protoplast by external enzyme hydrolysis method but to activate the degradation enzyme system itself. The dissociative protoplasts obtained by the method are high in quantity and are separated evenly, and the cell mass or tissue mass which is incompletely degraded is avoided.

Description

technical field [0001] The invention relates to a method for preparing protoplasts, in particular to a method for rapidly and intensively preparing protoplasts of Porphyra laver. Background technique [0002] Seaweed is delicious, high in protein, and contains essential amino acids and a large amount of flavor amino acids; vitamins A, B, C and iodine, calcium, iron, phosphorus, zinc, manganese, copper and other elements are also very rich; VA, Vb1, The content of Vb2 is comparable to that of animal liver. Seaweed has the effects of lowering serum cholesterol, preventing arteriosclerosis, nourishing the kidney, diuresis, cooling, calming the mind, night blindness, and developmental disorders on the human body. [0003] As early as the Sui and Tang Dynasties, there were records of eating seaweed in my country more than a thousand years ago. Our people have a long history of using and cultivating seaweed, and have accumulated rich experience. Now there is a great demand for se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01G33/02
Inventor 黄冰心丁兰平陈伟洲连绍兴谭华强马元元颜泽伟王展
Owner SHANTOU UNIV
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