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A kind of harmless treatment method of chlortetracycline solid waste

A solid waste, harmless treatment technology, applied in the field of environmental microorganisms and solid waste treatment, can solve the problems of high chlortetracycline residue, strong biological toxicity, and difficult degradation, and achieves easy control and operation of the fermentation process. Simple, acid-reducing effect

Inactive Publication Date: 2015-08-19
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of high residual amount of aureomycin in the solid waste of chlortetracycline residue, strong biological toxicity, high difficulty in degradation and high processing cost of the currently used method, the present invention aims to provide a method for harmless treatment of chlortetracycline residue by using microorganisms. treatment method

Method used

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  • A kind of harmless treatment method of chlortetracycline solid waste
  • A kind of harmless treatment method of chlortetracycline solid waste
  • A kind of harmless treatment method of chlortetracycline solid waste

Examples

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Embodiment 1

[0030] The main component determination of embodiment 1 aureomycin fresh bacterium residue

[0031] The initial water content, pH value and chlortetracycline residual amount of the fresh bacterium residue of chlortetracycline have been measured, know that the fresh bacterium residue initial water content of chlortetracycline≤80% (60-80%) (weight ratio ), the pH is 2.0-3.0, and the aureomycin content is 0.5-2.5% (on a dry basis).

[0032] Determination method of water content: adopt 105 ℃ drying method.

[0033] pH value measurement method: use acidity meter to measure.

[0034] Determination method of aureomycin content: high performance liquid chromatography (HPLC) method. The specific operation is: accurately weigh 2.0000g of bacteria residue, grind it, add 20mL of acetone extract, ultrasonicate for 30min, and filter with a 0.22μm filter membrane. The content of aureomycin in the filtrate was measured by high performance liquid chromatography and the degradation rate was ...

Embodiment 2

[0037] A kind of chlortetracycline slag biological treatment method of embodiment 2

[0038] The method mainly includes the following steps:

[0039] 1. Degradation strain selection and strain activation culture

[0040]According to the characteristics of the low pH value of the fresh bacterium residue of aureomycin and the high residual amount of aureomycin, Trichoderma harzii (Hypocrea lixii), Penicillium oxalicum (Penicillium oxalicum), Penicillium citrinum (Penicillium citrinum) and Penicillium punctatus (Penicillium spinulosu) and other aureomycin-degrading strains, such as Hypocrea lixii LJ245 (CGMCC NO.5753), Penicillium citrinum LJ318 (CGMCC NO.5850), Penicillium oxalicum LJ302 (CGMCC NO.7019) and Penicillium spinulosu LJ220 (CGMCC NO.5754) as preferred strains. Prepare Martin's Medium or Potato Glucose Medium, and activate the strains. The culture conditions are 28-30°C, 150-180r / min shaking culture for 24-48h, and then transfer to a larger container to continue the...

Embodiment 3

[0061] A kind of chlortetracycline slag biological treatment method of embodiment 3

[0062] The method mainly includes the following steps:

[0063] 1. Strain preparation

[0064] Inoculate the microbial strains that degrade chlortetracycline, such as Penicillium punctatus, Penicillium citrinum, Trichoderma harthorne and Penicillium oxalicum, in Martin's medium or potato dextrose medium, placed at 28-30°C, 150 Cultivate for 24-48h under the condition of -170r / min shaking, and then transfer to a container with a larger volume to continue culturing, and operate in this way for 2-4 generations. The preparation method of Martin's medium and potato glucose medium is the same as that in Example 2.

[0065] 2. Pretreatment of fungus residue raw materials

[0066] Break the fresh chlortetracycline residue from bulk particles into 1-5mm sized particles. Then put it into a high-pressure steam sterilizer and treat it at 121°C for 20min-30min. The bacteria residue without high tempe...

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Abstract

The invention relates to a method for harmlessly treating chlorotetracycline solid waste by microorganism, belonging to the technical field of environmental microorganism and solid waste treatment. According to the method, chlorotetracycline degrading microorganism is selected, chlorotetracycline dregs are employed as the main material, and a proper amount of specific auxiliary material is added, so that the chlorotetracycline and other biological toxic substances in the dregs can be degraded through fermentation treatment so as to achieve the harmless treatment effect of the dregs. The method for harmlessly treating chlorotetracycline solid waste by microorganism has the advantages of low cost, mild reaction condition, simplicity in operation, low energy consumption, easily controlled fermentation process, environment protection, high degradation efficiency and the like. Through the application of the method for harmlessly treating chlorotetracycline solid waste by microorganism, the biological detoxification problem of the chlorotetracycline dreg is solved, and a method and a basis are provided for harmless treatment and resource utilization of the solid waste.

Description

technical field [0001] The invention relates to a method for harmlessly treating aureomycin-containing solid waste by using microorganisms, and belongs to the technical field of environmental microorganism and solid waste treatment. Background technique [0002] Chlortetracycline (Chlortetracycline) belongs to the tetracycline antibiotics, is a broad-spectrum antibiotics, has inhibitory effect on a variety of Gram-positive and negative bacteria, spirochetes, rickettsia, mycoplasma, chlamydia, etc., widely used in livestock industries such as poultry farming and aquaculture. After being metabolized by livestock and poultry, most of these antibiotics are excreted into the environment in the form of prototype or mother nucleus along with the manure of livestock and poultry, which will pose a potential hazard to the environment and human health. The environmental pollution and control of antibiotic residues in livestock and poultry manure have attracted increasing attention. O...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02A62D101/28
Inventor 杨正礼李艳菊张爱平杨世琦张晴雯
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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