A kind of method for preparing water-soluble chitosan oligosaccharide

A technology for water-soluble chitosan oligosaccharide and chitosan, applied in the preparation of sugar derivatives, chemical instruments and methods, amino sugars, etc., can solve the problems of waste of raw materials, high risk, incomplete degradation process, etc. Cost, shortened irradiation time, complete effect of microwave degradation

Inactive Publication Date: 2015-08-26
SHANGHAI FENGYU BIOLOGICAL TECH DEVCO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a method for preparing water-soluble chitosan oligosaccharides, which solves the problems of incomplete degradation process, waste of raw materials, high cost and high risk of the purification process in the existing method.

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  • A kind of method for preparing water-soluble chitosan oligosaccharide

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Embodiment 1

[0014] Soak 1 kg of raw material polymer chitosan in pure water for 6 hours after preliminary treatment to form 1000 ml of chitosan and pure water mixture, and then place the soaked mixture on a microwave generator for tunnel-type continuous microwave Treatment can ensure that the soaked chitosan is subjected to uniform microwave radiation, wherein the time of microwave radiation is 5min, and the temperature of microwave radiation is 130°C; the solution obtained after microwave radiation is degraded with 3000ml of food-grade hydrogen peroxide. Distilled water is added during the degradation process of food-grade hydrogen peroxide. After the reaction is complete, nano-membrane filtration separates the crude chitosan oligosaccharide and the filtrate with low molecular weight. The obtained filtrate can be sent to the soaking process for reuse; the crude chitosan oligosaccharide is washed with pure water to remove impurities , high-speed centrifugation and concentration of the wash...

Embodiment 2

[0016] Soak 1 kg of raw material polymer chitosan in pure water for 8 hours after preliminary treatment to form 1000 ml of chitosan and pure water mixture, and then place the soaked mixture on a microwave generator for continuous tunneling. Microwave treatment can ensure that the soaked chitosan is subjected to uniform microwave radiation. The time of microwave radiation is 30 minutes, and the temperature of microwave radiation is 120°C; the solution obtained after microwave radiation is degraded with 2500ml of food-grade hydrogen peroxide. , Distilled water was added during the degradation process of food-grade hydrogen peroxide. After the reaction was complete, the crude chitosan oligosaccharide and the filtrate with low molecular weight were separated by nano-membrane filtration. The obtained filtrate could be sent to the soaking process for reuse; the crude chitosan oligosaccharide was removed by washing with pure water Impurities, high-speed centrifugation and concentratio...

Embodiment 3

[0018] Soak 1 kg of raw material polymer chitosan in pure water for 10 hours after preliminary treatment to form 1000 ml of chitosan and pure water mixture, and then place the soaked mixture on a microwave generator for continuous tunneling. Microwave treatment can ensure that the soaked chitosan is subjected to uniform microwave radiation. The time of microwave radiation is 60 minutes, and the temperature of microwave radiation is 100°C; the solution obtained after microwave radiation is degraded with 2000ml of food-grade hydrogen peroxide. , Distilled water is added during the degradation process of food-grade hydrogen peroxide. After the reaction is complete, the crude chitosan oligosaccharide with low molecular weight and the filtrate are separated by nano-membrane filtration. The obtained filtrate can be sent to the soaking process for reuse; the crude chitosan oligosaccharide is washed with pure water Remove impurities, centrifuge and concentrate the washed solution at hi...

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Abstract

A method for preparing water soluble chitosan oligosaccharide comprises the following steps: placing preliminarily treated raw material high molecular weight chitosan into a pure water and then placing a soaked mixed solution in a microwave generator so as to carry out tunnel type continuous microwave treatment on the mixed solution; degrading the obtained mixed solution treated by microwaves through using food-grade hydrogen peroxide,; and obtaining low molecular weight chitosan crude product and a filter liquor through nano membrane separation, wherein the filter liquor is fed into an initial soaking step to be continuously used and the chitosan crude product is purified to obtain a chitosan finished product. The method for preparing the water soluble chitosan oligosaccharide, provided by the invention, has the advantages as follows: due to the adoption of the tunnel type continuous microwave treatment, the chitosan soaked in the solution is uniformly radiated, so that the microwave radiation time is reduced and the microwave degradation degree is completer; and as a nano membrane filtering method replaces an alcohol precipitation method in the purifying process, potential threats caused by storage and the use of a great deal of ethanols are avoided and the technical cost is also reduced.

Description

technical field [0001] The invention belongs to the field of degradation of macromolecular sugars, in particular to a method for preparing water-soluble chitosan oligosaccharides. Background technique [0002] Chitosan oligosaccharide is also called chitosan oligosaccharide, also known as chitosan oligosaccharide, scientific name β-1,4-oligosaccharide-glucosamine, is a brand new product obtained by processing chitosan, and has good water solubility , Low molecular weight product with great function and high biological activity. It has many unique functions such as high solubility and easy absorption by organisms that chitosan does not have, and its effect is 14 times that of chitosan. [0003] At present, the preparation methods of chitosan oligosaccharide mainly include chemical method, enzymatic method and physical method. Among them, chemical methods mainly include acid degradation method and oxidative degradation method. Functional groups attack many free amino groups ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H5/06C07H1/00C08B37/08
Inventor 谷经建
Owner SHANGHAI FENGYU BIOLOGICAL TECH DEVCO LTD
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