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Preparation method of collagen film for guided tissue regeneration

A technology for guiding regeneration and collagen membrane, applied in medical science, surgery, etc., can solve the problems of low tensile strength of collagen membrane, loss of space maintenance ability, fast degradation speed, etc., and achieve excellent mechanical properties, excellent controllable degradation performance, good repair effect

Active Publication Date: 2014-09-03
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still some problems in the research of collagen film as GTR: first, the degradation rate in vivo is too fast, and second, compared with the requirements of GTR film, the tensile strength of collagen film is relatively small, and it is easy to collapse during application and lose the ability to maintain space

Method used

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  • Preparation method of collagen film for guided tissue regeneration
  • Preparation method of collagen film for guided tissue regeneration
  • Preparation method of collagen film for guided tissue regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for preparing a collagen membrane for guided tissue regeneration, comprising the steps of:

[0034] (1) Dissolve collagen in 0.1M acetic acid solution at 25°C to obtain a collagen solution with a concentration of 6mg / ml;

[0035] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 7 with 0.1M sodium hydroxide solution, and keep it for 30 minutes;

[0036] (3) shear and stir the collagen solution obtained in step (2) with an electric mixer at 3000r / min for 5 minutes to obtain a collagen emulsion;

[0037] (4) Centrifuge the emulsion in step (3) at 2000r / min for 8 minutes. At this time, the collagen floats on the surface of the solvent, and collect the collagen film on the solution;

[0038] (5) Forming the collagen film obtained in step (4), then freeze-drying, and vacuum thermal crosslinking at 120°C;

[0039] (6) Packaging, irradiation sterilization.

[0040] Sample tear strength: 31.0MPa.

Embodiment 2

[0042] A method for preparing a collagen membrane for guided tissue regeneration, comprising the steps of:

[0043] (1) Dissolve collagen in 0.1M hydrochloric acid solution at 30°C to obtain a collagen solution with a concentration of 5mg / ml;

[0044] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 5 with 0.1M sodium hydroxide solution, and keep it for 10 minutes;

[0045] (3) The collagen solution in step (2) is sheared and stirred for 5 seconds under the condition of 15000 r / min with a tissue masher to obtain a collagen emulsion;

[0046] (4) Centrifuge the emulsion in step (3) at 1000r / min for 10 minutes. At this time, the collagen floats on the surface of the solvent, and collect the collagen film on the solution;

[0047] (5) Forming the collagen film obtained in step (4), freeze-drying at -20°C, and thermally crosslinking under vacuum at 140°C;

[0048] (6) Soak the collagen membrane obtained in step (5) in 1% glutaraldehyde solution for...

Embodiment 3

[0052] A method for preparing a collagen membrane for guided tissue regeneration, comprising the steps of:

[0053] (1) Dissolve collagen in 0.1M hydrochloric acid solution at 4°C to obtain a collagen solution with a concentration of 10mg / ml;

[0054] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 8 with 0.1M ammonia water, and keep it for 10 minutes;

[0055] (3) The collagen solution obtained in step (2) is sheared and stirred for 15 seconds under the condition of 15000 r / min with a tissue masher to obtain a collagen emulsion;

[0056] (4) Centrifuge the emulsion in step (3) at 6000r / min for 5 minutes. At this time, the collagen floats on the surface of the solvent, and collect the collagen film on the solution;

[0057] (5) Forming the collagen film obtained in step (4), freeze-drying at -20°C, and vacuum thermal crosslinking at 110°C;

[0058] (6) Put the collagen film obtained in step (5) into 1% EDC-NHS solution (weigh a certain amount ac...

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Abstract

The invention discloses a preparation method of a collagen film for guiding tissue regeneration. The method comprises the following steps: dissolving the collagen in the acidic solution; adjusting the pH value of the obtained solution to 5-9; continuing to rapidly shear and stir the obtained solution to obtain a collagen emulsion; centrifuging the obtained collagen emulsion to collect the collagen film on the solution; The obtained collagen film is molded, freeze-dried, and cross-linked by physical or / and chemical methods. The collagen film obtained by the present invention has excellent mechanical properties and controllable degradation properties, and has a gradient pore structure, which is suitable for the transmission of nutrients and the adsorption and proliferation of cells, and can better guide the repair of defective tissues; the preparation of the present invention The process is simple, the cost is low, and it is beneficial to large-scale production.

Description

technical field [0001] The invention relates to a preparation method of a collagen film for guiding tissue regeneration. Background technique [0002] Periodontal guided tissue regeneration (guided tissue regeneration, GTR) is currently the most advanced technology in the treatment of periodontal disease in the world. In 1982, Nymans et al. first proposed the concept of GTR (Nyman S.Lindhe J.Karring T, et al. Clin Periodontol.1982; 9(4):290-296). The core of GTR technology is the tissue-guided regeneration membrane, that is, by guiding The membrane acts as a space-isolated barrier membrane, while preventing gingival connective tissue cells and junctional epithelial cells from migrating to the root, while selectively allowing periodontal ligament cells and alveolar bone cells to adhere to the root surface and differentiate to form new periodontal attachments. This approach has been shown to be more effective in achieving clinical attachment and reducing probing depth than op...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04
Inventor 任力季培红秦晓杰王迎军刘卅
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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