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Method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid

An arabinoxylan, high-purity technology, applied in the field of comprehensive utilization of biomass, can solve the problems of affecting the extraction efficiency of active substances, easily destroying functional substances, low product purity, etc., so as to improve the utilization rate of raw materials and improve the functionality of products. , the effect of improving economic efficiency

Inactive Publication Date: 2013-04-17
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the particularity of the cellulosic materials, the traditional extraction of phenolic acids and functional oligosaccharides uses strong chemical methods such as acid or alkali methods to treat the cellulosic raw materials, which is very easy to destroy the functional substances in the raw materials.
However, enzymatic extraction of functional substances generally faces the problem of low extraction rate, so how to effectively pretreat raw materials directly affects the extraction efficiency of active substances
And the final product also faces the problem of low product purity, resulting in low activity

Method used

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  • Method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) The bran is dried to a moisture content of less than 5%, firstly pulverized by a pulverizer, then passed through a 40-mesh sieve, and then pulverized by a mechanical ultrafine pulverizer to a size of 280 mesh;

[0026] (2) The superfine bran prepared in step (1) was prepared into a water suspension according to the mass ratio of 1:8, and 0.2 mL of high-temperature amylase was added to boil for 1 hour. After cooling to 60 ° C, the pH was adjusted to 8.5, and alkaline protease was added. Incubate at 50°C for 0.5h, then adjust the pH to 4.2, add glucoamylase to react for 0.5h, centrifuge at 3600 rpm for 20min, discard the supernatant, and wash the filter residue 3 times with hot distilled water;

[0027] (3) The filter residue after washing is prepared into a suspension with deionized water at a mass ratio of 1:6, and the enzyme preparation is added in an amount of 2% (on a dry basis) by adding xylanase and ferulic acid esterase in a mass ratio Composite enzyme prepara...

Embodiment 2

[0034] (1) The bran is dried to a moisture content of less than 5%, firstly crushed by a pulverizer, then passed through a 40-mesh sieve, and then crushed by a ball mill to 300 mesh;

[0035](2) The ultra-fine bran prepared in step (1) was prepared into a water suspension according to the mass ratio of 1:10, and 0.25 mL of high-temperature amylase was added to boil for 1.5 hours. After cooling to 60 ° C, the pH was adjusted to 8.8, and alkaline Incubate the protease at 50°C for 1 hour, then adjust the pH to 4.5, add glucoamylase to react for 0.5 hour, centrifuge at 3600 rpm for 20 minutes, discard the supernatant, and wash the filter residue 3 times with hot distilled water;

[0036] (3) The filter residue after washing was prepared into a suspension with deionized water at a mass ratio of 1:10, and the enzyme preparation was added at a mass ratio of 1.8% (on a dry basis) by adding xylanase and ferulic acid esterase 1:3.5 compound enzyme preparation, at the same time, use ultr...

Embodiment 3

[0043] (1) The bran is dried to a moisture content of less than 5%, firstly crushed by a pulverizer, then passed through a 40-mesh sieve, and pulverized by a jet mill to 300 mesh;

[0044] (2) The superfine bran prepared in step (1) was prepared into a water suspension according to the mass ratio of 1:8, and 0.22 mL of high-temperature amylase was added to boil for 1 hour. After cooling to 60°C, the pH was adjusted to 8.0, and alkaline protease was added. Incubate at 50°C for 0.5 h, then adjust the pH to 4.4, add glucoamylase for 1 h, centrifuge at 3600 rpm for 20 min, discard the supernatant, and wash the filter residue 4 times with hot distilled water;

[0045] (3) The filter residue after washing is prepared into a suspension with deionized water at a mass ratio of 1:9, and the enzyme preparation is added in an amount of 1.5% (on a dry basis) by adding xylanase and ferulic acid esterase in a mass ratio Composite enzyme preparation with a composition of 1:3, while using ultr...

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Abstract

The invention relates to a method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid and belongs to the field of biomass comprehensive utilization. According to the invention, the bran of wheat and corns are subjected to efficient pre-processing and an ultrasonic assisted composite enzymolysis is adopted so as to obtain a mixed liquid of the araboxylan and the ferulic acid, and then the mixed liquid flows through absorbent carbon to be subjected to adsorption and separation; the araboxylan solution is subjected to bleaching through the combination of absorbent carbon particles and bleaching resin, and subjected to chromatographic fractionation through a sequential simulated moving bed, so as to obtain the low-poly araboxylan product with oligosaccharide purity higher than 95 percent; and the ferulic acid is subjected to ethanol leaching to obtain the high-purity ferulic acid product. According to the invention, the bran, by-products of grain processing, are taken as raw materials for deep processing and comprehensive utilization, thereby obtaining two products which have high added value, are high in purity and good in functionality; and accordingly, the food raw material utilization rate is effectively improved, and the added value of the deep processing industry of the food is increased.

Description

technical field [0001] The invention relates to a method for producing high-purity oligomeric arabinoxylan and ferulic acid by using bran as raw materials, belonging to the field of comprehensive utilization of biomass. Background technique [0002] During the deep processing of corn and wheat, a large number of by-products, such as wheat bran and corn bran, will be produced. Taking corn as raw material to produce corn starch as an example, in the process of corn starch processing, the effective utilization part only accounts for about 70% of corn, and 30% of the by-products cannot be well utilized. More than 20 million tons will be produced every year, so the rational utilization of processing by-products is particularly important. However, due to the unique smell, color and taste of bran, and the unbalanced nutrient content, it lacks some essential nutrients for the human body, so the edible and nutritional value is low, which limits the utilization of these by-products. ...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12P19/20C12P19/14
Inventor 刘宗利王乃强王彩梅
Owner BAOLINGBAO BIOLOGY
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