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Electrochemical DNA sensor based on graphene-precious metal composite and preparation method thereof

A technology of composite materials and precious metals, applied in the field of electrochemistry and material synthesis, can solve the problems of small particles, low current density, and inability to pack tightly

Inactive Publication Date: 2013-04-10
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is true that nano-noble metals have high electrocatalytic properties, but the biggest disadvantage of nano-materials is that they cannot be packed as closely as micron-scale materials on electrodes because of their small size, resulting in less current density, so the sensitivity and specificity of the sensor poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment （ 1

[0022] Add 25g of concentrated sulfuric acid, 2.4g potassium persulfate, 2.5g phosphorus pentoxide in a 500mL container, stir, slowly warm up to 80℃ in a water bath, add 5.0g graphite, stir for 10 hours, and cool to room temperature naturally. After suction filtration and drying, pre-oxidized graphite is obtained. Add 2.5g of pre-oxidized graphite, 1.3g of sodium nitrite, and 105g of concentrated sulfuric acid into the flask, stir under ice bath, slowly add 7.5g of potassium permanganate, control the temperature at 0-5℃ and react for 1 hour, then switch to 35 Heat in a water bath at ℃, keep the temperature for 8 hours, remove the water bath and add distilled water (T <98℃), heat in a water bath to 96℃, keep warm for 30 minutes, add 7.4g 5% hydrochloric acid and 5mL 30% hydrogen peroxide to the bottle when the temperature drops to 50-60℃, wash with distilled water to neutral, ultrasonic peeling, dialysis , Dry to obtain graphene oxide.

[0023] Weigh 20mg of graphene oxide into 2...

Embodiment （ 2

[0026] Add 25g of concentrated sulfuric acid, 2.4g potassium persulfate, 2.5g phosphorus pentoxide in a 500mL container, stir, slowly warm up to 80℃ in a water bath, add 5.0g graphite, stir for 10 hours, and cool to room temperature naturally. After suction filtration and drying, pre-oxidized graphite is obtained. Add 2.5g of pre-oxidized graphite, 1.3g of sodium nitrite, and 105g of concentrated sulfuric acid into the flask, stir under ice bath, slowly add 7.5g of potassium permanganate, control the temperature at 0-5℃ and react for 1 hour, then switch to 35 Heat in a water bath at ℃, keep the temperature for 8 hours, remove the water bath and add distilled water (T <98℃), heat in a water bath to 96℃, keep warm for 30 minutes, add 7.4g 5% hydrochloric acid and 5mL 30% hydrogen peroxide to the bottle when the temperature drops to 50-60℃, wash with distilled water to neutral, ultrasonic peeling, dialysis , Dry to obtain graphene oxide.

[0027] Weigh 20mg of graphene oxide in 20m...

Embodiment （ 3

[0030] Add 25g of concentrated sulfuric acid, 2.4g potassium persulfate, 2.5g phosphorus pentoxide in a 500mL container, stir, slowly warm up to 80℃ in a water bath, add 5.0g graphite, stir for 10 hours, and cool to room temperature naturally. After suction filtration and drying, pre-oxidized graphite is obtained. Add 2.5g of pre-oxidized graphite, 1.3g of sodium nitrite, and 105g of concentrated sulfuric acid into the flask, stir under ice bath, slowly add 7.5g of potassium permanganate, control the temperature at 0-5℃ and react for 1 hour, then switch to 35 Heat in a water bath at ℃, keep the temperature for 8 hours, remove the water bath and add distilled water (T <98℃), heat in a water bath to 96℃, keep warm for 30 minutes, add 7.4g 5% hydrochloric acid and 5mL 30% hydrogen peroxide to the bottle when the temperature drops to 50-60℃, wash with distilled water to neutral, ultrasonic peeling, dialysis , Dry to obtain graphene oxide.

[0031] Weigh 20mg of graphene oxide into 2...

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Abstract

The invention relates to an electrochemical DNA sensor based on a graphene-precious metal composite and a preparation method thereof. The preparation method comprises the following steps: (1) preparing an aqueous dispersion of graphene oxide from graphite powder by utilizing a Hummer method; (2) preparing a nano-precious metal particle / graphene composite by a chemical reduction method; and (3) adhering the precious metal / graphene composite onto a glassy carbon electrode, modifying with single-stranded DNA, and hybridizing the modified electrode and a target gene so as to obtain the electrochemical DNA sensor. According to the electrochemical DNA sensor based on a graphene-precious metal composite, a detection limit of DNA can be greatly increased to 1.2*10<-10>mol / L.

Description

Technical field [0001] The invention relates to an electrochemical DNA biosensor and a preparation method of electrode materials used therein, belonging to the technical field of electrochemistry and material synthesis. Background technique [0002] Biosensor is currently the most rapidly developing detection device and is considered the most promising for industrialization. It has the advantages of high specificity, short-time and low-cost analysis, no special requirements for analyte substances, safe operation, and convenient on-site determination. Since Clark and Lyons first proposed the idea of ​​biosensor in 1962, it has developed vigorously and rapidly in recent decades. It has been widely used in various sectors of the national economy, such as food, pharmaceuticals, chemicals, clinical testing, biomedicine, and environmental monitoring. [0003] With the deepening of molecular biology research, the detection methods of deoxyribonucleic acid DNA have become more and more i...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 秦勇储富强陶永新孔泳黎珊
Owner CHANGZHOU UNIV
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