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Bactrian camel lactoferrin gene, recombinant protein and cloning method thereof

A lactoferrin and recombinant protein technology, applied in the field of genetic engineering, can solve the problem of little research on Bactrian camel lactoferrin, achieve the effect of inhibiting tumor cells and disease treatment, enhancing the body's immune resistance to disease, and promoting bone marrow cells. generated effect

Inactive Publication Date: 2013-04-03
XINJIANG WANGYUAN CAMEL MILK IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The content of lactoferrin in Bactrian camel milk from Alxa League in Inner Mongolia is significantly higher than that in cow milk, and lactoferrin has very important biological functions. little research on protein

Method used

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  • Bactrian camel lactoferrin gene, recombinant protein and cloning method thereof
  • Bactrian camel lactoferrin gene, recombinant protein and cloning method thereof
  • Bactrian camel lactoferrin gene, recombinant protein and cloning method thereof

Examples

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Embodiment 1

[0026] Embodiment 1: A Bactrian camel lactoferrin gene, which is a gene closely related to the synthesis of non-heme iron-binding glycoproteins that control the transferrin family, has the nucleotide sequence from SEQ ID NO.1 The nucleotide sequence of position 22-2104 has 90% similarity with the nucleotide sequence of dromedary lactoferrin AJ131674.1.

Embodiment 2

[0027] Example 2: A recombinant protein of Bactrian camel lactoferrin gene.

Embodiment 3

[0028] Example 3: The nucleotide sequence of the recombinant protein is SEQ ID NO.1.

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Abstract

The invention relates to the technical field of genetic engineering and discloses a bactrian camel lysozyme protein gene, a recombinant protein and a cloning method thereof. The gene is a gene which is closely related to a gene related to glycoprotein synthesis of controlling nonheme iron associative property in the transferring family. The bactrian camel lysozyme protein gene and an evolutionary tree thereof can intuitively prove the difference between the bactrian camel lysozyme protein sequence and the protein sequence of other species, experimental data are provided for the functions of researching transfer of bactrian camel iron, realizing the broad-spectrum antibacterial and anti-virus function, regulating the in-vivo iron balance, promoting myelopoiesis, promoting cell growth, enhancing the body immunity and disease resistance and suppressing the tumor cells and disease treatment, a certain theoretical basis is provided for researching regulation of certain inflammatory responses and body immunity systems of the bactrian camel, other species and humans, and a certain biological basis is provided for research of body immune defense system; and therefore, the bactrian camel lysozyme protein gene has high application value.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, and relates to a Bactrian camel lactoferrin gene, a recombinant protein and a cloning method thereof. Background technique [0002] Lactoferrin (lactoferrin, LF) is a non-heme iron-binding glycoprotein, which belongs to the transferrin family. It is widely distributed in various tissues and exocrine fluids of humans, mammals and livestock. Studies have found that LF is a protein with multiple biological functions. It is not only involved in the transport of iron, but also found that lactoferrin has a wider range of biological functions, including broad-spectrum antibacterial, antiviral, and regulation of iron in the body. Balance, promote bone marrow cell production, promote cell growth, enhance the body's immune and disease resistance, inhibit tumor cells, and can work synergistically with a variety of antibiotics and antifungal agents to treat diseases more effectively, participate...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/79C12N15/10
Inventor 陈钢粮
Owner XINJIANG WANGYUAN CAMEL MILK IND
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