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Method for extracting acetoin from fermentation liquor

A technology of acetoin and fermentation liquid, which is applied in the field of biological separation, can solve the problems of difficult separation of acetoin downstream, and achieve the effects of easy regeneration, good adsorption selectivity, and mild desorption conditions

Inactive Publication Date: 2014-07-30
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a method for extracting acetoin from a fermentation broth mainly containing ethanol, acetoin and acetic acid, so as to solve the difficult problem of downstream separation of acetoin in the fermentation broth and reduce the Energy consumption in the separation process of couple marriage

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  • Method for extracting acetoin from fermentation liquor
  • Method for extracting acetoin from fermentation liquor
  • Method for extracting acetoin from fermentation liquor

Examples

Experimental program
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Effect test

Embodiment 1

[0039] In the fermented liquid obtained by Fusobacterium acetobutylicum B3 (CGMCC 5234) fermented, the percentage composition of acetoin is 0.2% (w / w), and its fermentation process is as follows: (1) preparation plate culture medium: glucose 10g / L, yeast extract 5g / L, peptone 3g / L, magnesium sulfate heptahydrate 3g / L, ammonium acetate 2g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, agar 15g / L, at 121 Sterilize at ℃ for 15 minutes. Then the strains were streaked on a solid plate and cultured in a Bugbox anaerobic box (Ruskinn, UK) at 37°C for 24 hours, white irregular colonies grew on the plate. (2) Preparation of seed medium: the same formula as plate medium, but without adding agar. Then pick the bacteria sludge on a plate, inoculate it in 150ml seed medium, and culture it statically at 37°C for 12h. (3) Preparation of fermentation medium: glucose 60g / L, ammonium acetate 2.5g / L, magnesium sulfate heptahydrate 0.5g / L, potassium dihydrogen pho...

Embodiment 2

[0042] The fermented liquid obtained in Example 1 is first coarsely filtered, then in a centrifuge at 12000rpm, centrifuged for 2min, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 2000 to 10000 Daltons to remove bacteria and some Miscellaneous protein, at this moment, main components such as acetone, ethanol, butanol, acetoin, acetic acid, butyric acid are contained in the fermented liquid. Pass the obtained fermentation broth through a continuous separation column device equipped with AX-1 macroporous adsorption resin. This device is a four-zone simulated moving bed separation device. pump in for adsorption; the impurity washing area uses deionized water to pump in at a flow rate of 3-20mL / min for impurity washing; the eluting area uses 40%-100% (w / w) methanol solution as the eluent and Pump in at a flow rate of ~25ml / min for elution; in the regeneration zone, use deionized water as a regenerant to pump in at a flow rate of 5~25ml / ...

Embodiment 3

[0044] In a 2000mL three-necked flask, add 200g of dry chloromethylated polystyrene resin, add 1200g of o-nitroethylbenzene, stir and mix, fully swell at room temperature for more than 3h, then add 30g of ferric chloride and stir for 2h, and gradually heat up to 130 ~160°C, carry out Friedel-Crafts post-crosslinking reaction, control the residual chlorine content at 1~2wt%, and stop the reaction. After cooling, suck out the mother liquor, and repeatedly extract the resin with industrial ethanol until the extraction effluent is colorless and clear. Vacuum drying to obtain medium polar ultra-high cross-linked resin HD-02.

[0045] The obtained product is in the shape of brownish-red round particles, with an average particle size of 0.52mm, a water content of 43%, an average pore size of 1.9nm, and a specific surface area of ​​1645.5m 2 / g, the average pore volume is 0.796cm 3 / g. The scanning electron micrograph of the synthesized resin is shown in figure 1 , BET diagram (a)...

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Abstract

The invention discloses a method for extracting acetoin from fermentation liquor. Pretreated acetoin fermentation liquor is conducted to adsorption, impurity washing and elution through an adsorption column in which sorbent is contained, eluant is collected, and then acetoin solution is obtained, wherein the sorbent adopts hyper-crosslinked resin HD-02; the framework of the resin adopts polystyrene; polystyrene is conducted to Friedel-Crafts and then is prepared into the acetoin after cross linking reaction; and the acetoin has medium polarity, with the percent of void being 40 to 50 percent, the particle size being 0.42 to 0.55 mm, the wet density being 1.05 to 2.02g / cm<3>, the average specific surface being 1450 to 1800m<2> / g, the average pore diameter being 1.62 to 2.70 nm, the pore volume being 0.73 to 0.86 cm<3> / g, and the water content being 42 to 45 percent. The method is simple and efficient, the energy consumption is low, and the acetoin is effectively separated from the fermentation liquor.

Description

technical field [0001] The technical field of bioseparation of the present invention particularly relates to a method for extracting acetoin from fermentation broth. Background technique [0002] Acetoin (also known as: 3-hydroxy-2-butanone) is a widely used and lovable food flavor, and also has important applications in the pharmaceutical industry, chemical industry, coating industry, and IT industry. The global demand for acetoin is about 1000t, and the price of natural acetoin in the international market is about 300,000 yuan / ton. At present, acetoin fragrance products in the international market are mainly produced by several large companies in the United States, Germany and Japan, and they are mainly synthetic products. However, there are shortcomings such as low product yield and yield in the synthetic method, and serious environmental pollution, and the product quality is difficult to meet the requirements of the largest consumption field of acetoin—edible spices; T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/17C07C45/79
Inventor 应汉杰王丽丽吴菁岚周精卫林晓清焦鹏飞张旭
Owner NANJING TECH UNIV
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