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Method for preparing xanthohumol from European hop spike

A technology of xanthohumol and hops, which is applied in the field of preparation of xanthohumol, can solve the problems of high industrialization cost, serious environmental pollution, and large safety hazards, and achieve the effects of product quality and safety, high degree of industrialization, and reduced decomposition

Inactive Publication Date: 2013-02-06
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] 1. Using macroporous resin for purification, it is easy to contaminate the product, and the product safety is poor
[0012] 2. Using supercritical extraction, the industrialization cost is high
[0013] 3. It is difficult to mass-produce by silica gel column or high-speed countercurrent chromatography
[0014] 4. Extraction with organic reagents, especially flammable and explosive reagents such as petroleum ether, acetone, chloroform, ethyl acetate, etc., has great potential safety hazards, serious environmental pollution, and difficult control of solvent residues

Method used

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  • Method for preparing xanthohumol from European hop spike

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The hops are crushed, put 10kg into the extraction tank, add 10 times of 70% ethanol to soak for 1 hour, extract twice, the extract is decompressed to recover ethanol, stand for 12 hours to filter, and add a hollow cellulose ultrafiltration membrane system with a molecular weight cut-off of 3000 Medium and ultrafiltration, the permeate is added to the cut-off 300 nanofiltration membrane system to concentrate, the concentrated solution is added to 500ml polyamide resin for adsorption, and eluted with 4L15% ethanol solution, the eluate is collected and concentrated under reduced pressure to extract. Water and ethanol were refluxed to dissolve, refrigerated to crystallize, and dried in vacuum to obtain 34 g of yellow powder, which was detected to have a content of 93.4%.

Embodiment 2

[0025] The hops are crushed, put 10kg into the extraction tank, add 6 times of 50% ethanol to soak for 2 hours, extract 3 times, the extract is decompressed to recover ethanol, stand for 8 hours to filter, add hollow cellulose ultrafiltration membrane system with molecular weight cut off 6000 In ultrafiltration, the permeate is added to the cut-off 200 nanofiltration membrane system to concentrate, the concentrated solution is added to 500ml polyamide resin for adsorption, and eluted with 2.5L 25% ethanol solution, and the eluate is collected and concentrated under reduced pressure to extract. Dissolve under reflux with absolute ethanol, refrigerate and crystallize, and vacuum-dry to obtain 45 g of yellow powder, the content of which is 80.1% after testing.

Embodiment 3

[0027] The hops are crushed, put 10kg into the extraction tank, add 10 times of 60% ethanol to soak for 1 hour, extract twice, the extract is decompressed to recover ethanol, stand for 10 hours to filter, and add hollow cellulose ultrafiltration membrane system with a molecular weight cut off of 10000 In ultrafiltration, the permeate is added to the cut-off 300 nanofiltration membrane system to concentrate, the concentrated solution is added to 500ml polyamide resin for adsorption, and eluted with 3L of 20% ethanol solution, and the eluate is collected and concentrated under reduced pressure to extract. The water and ethanol were refluxed to dissolve, refrigerated and crystallized, and vacuum-dried to obtain 38g of yellow powder, the content of which was detected to be 85.2%.

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Abstract

The invention belongs to the technical field of biology, and especially relates to a method for preparing xanthohumol from European hop spike. The method is characterized in that an European hop spike medicine material is crushed; 5-10 time amount of 50-70% ethanol is added for carrying out warm-soaking extraction 2-3 times; ethanol in an obtained extraction liquid is recovered, and the extraction liquid is filtered in an ultrafiltration membrane system; a permeate is concentrated in a nano-filtration membrane system; the concentrate is absorbed by using polyamide resin; 5-8 time amount of 15-25% ethanol is used for eluting; the elution liquid is concentrated into a paste; dehydrated ethanol is added for reflux dissolving; refrigeration crystallization is carried out; and a crystalline material is dried, such that xanthohumol is prepared. The method provided by the invention has the advantages of simple process, easy operation, low energy consumption during the production process, low pollution, and safe quality of obtained product. The product can be directly used in productions of foodstuffs and medicines.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for preparing xanthohumol from hops. Background technique [0002] Hops belong to the genus Cannabis, also known as Hubu, Hops, Yeast Flowers, and Hops. It is called hop flower in "Compendium of Materia Medica", and the ancients took it as a medicinal material. In 1079, the Germans first added hops when brewing beer, so that the beer had a refreshing bitter taste and a fragrant aroma. Since then, hops have been known as the "soul of beer" and have become one of the indispensable raw materials for beer brewing. It makes beer have unique bitterness and aroma, and has the ability to preserve and clarify wort. [0003] Modern studies have shown that hops contain flavonoids, polyphenols, and volatile oils, which have good antibacterial, anti-ulcer, anti-thrombotic, hypotensive, anti-allergic, anti-cancer, anti-dementia, and prevention of osteoporosis and many other effects. S...

Claims

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Application Information

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IPC IPC(8): C07C49/84C07C45/78
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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