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Pyrethroid pesticide degrading bacteria, separation and purification method thereof and application thereof

A technology for the degradation of pyrethroids and pesticides, which can be used in chemical instruments and methods, biochemical equipment and methods, and microorganism-based methods. Effect

Active Publication Date: 2013-01-30
路亚科消防车辆制造有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on microbial degradation of pesticides is mainly concentrated in the environment of soil, fruit and freshwater sediments, while there are few studies on the screening of degrading bacteria for pyrethroid pesticide residues in offshore environments.

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  • Pyrethroid pesticide degrading bacteria, separation and purification method thereof and application thereof
  • Pyrethroid pesticide degrading bacteria, separation and purification method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0023] Screening of Degrading Pyrethroid Pesticide Strains

[0024] 1. Media and reagents

[0025] Nutrient medium: 5.0 g of peptone, 0.01 g of ferric phosphate, 1.0 g of yeast extract, 1000 mL of filtered seawater, pH 7.6-7.8, prepared after high-pressure steam sterilization (121°C, 20 min);

[0026] Enrichment culture solution: cypermethrin, deltamethrin, fenvalerate, cyfluthrin and bifenthrin were respectively added 1.5 times the weight of emulsifier Tween-20 to prepare corresponding thick emulsion, and then Each concentrated emulsion was added to the nutrient medium respectively, so that the final concentrations of cypermethrin, deltamethrin, fenvalerate, cyfluthrin and bifenthrin were respectively 100 mg / L to obtain enriched culture solution.

[0027] 2. Isolation and purification of bacterial strains

[0028] Seawater samples were collected from the coastal waters of Ningbo, and 500 mL of seawater was filtered under sterile conditions. After enrichment, it was added to...

specific Embodiment 2

[0032] Identification of strains degrading pyrethroid pesticides

[0033] The bacterial strain obtained above is carried out morphological characteristics and molecular biological identification, and the scanning electron micrograph of this bacterial strain is as follows: figure 1 shown. The main biological characteristics of the strain are: Gram stain negative, bacillus, arginine double hydrolysis test, lysine decarboxylase test, V-P reaction, gelatin liquefaction test positive, β-galactosidase, ornithine Decarboxylase, tryptophan deaminase test, urease test, indole test, H 2S reactions were all negative, and sucrose could not be utilized. The strain grows in seawater at 25-30°C, and the optimum growth pH is 7.0-7.8. On solid medium, the colonies are round, thin, white and translucent. The strain was identified by 16S rRNA sequence analysis as having the highest similarity with Methylophaga sp., reaching 98%.

specific Embodiment 3

[0034] Degradation Effect and Application of Pyrethroid Pesticide-degrading Bacteria on Pyrethroid Pesticides in Seawater

[0035] The isolated and purified strains were measured by bacterial count OD 415nm =0.2 Inoculated into 100 mL of 100 mL enrichment medium containing 100 mg / L of cypermethrin, deltamethrin, fenvalerate, cyfluthrin, and bifenthrin ((the preparation method is as shown in Example 1)) 250 In a 3 mL Erlenmeyer flask, the enrichment medium without inoculation was used as a control, and cultured on a constant temperature shaker at 28 °C and 180 rpm for 7 days. After the cultivation, draw 1 mL of the culture solution, add 0.4 g of NaCl, vortex and mix well, and then use 4 mL of the mixed solution of n-hexane and acetone (volume ratio 1:1) and 2 mL of the mixed solution of n-hexane and acetone Extract, combine the supernatants obtained from the two extractions, take 3 mL supernatant, add 0.4 g anhydrous sodium sulfate, blow dry with nitrogen, and dissolve with 1 ...

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Abstract

The invention discloses a pyrethroid pesticide degrading bacteria, wherein the bacteria is an HS-24 strain, with a classification naming as Methylophagasp., and is preserved in China General Microbiological Culture Collection Center on April 24, 2012, an accession number being CGMCCNo. 6046; and a Genbank accession number of the 16SrRNA of the strain is JX087925. The invention simultaneously discloses a separation and purification method and an application of the pyrethroid pesticide degrading bacteria. The invention is advantageous in that the bacteria can safely, highly-efficiently and rapidly degrade 5 kinds of pyrethroid pesticide: cypermethrin, deltamethrin, fenvalerate, cyfluthrin and bifenthrin in a marine environment, and has a good application prospect in the aspects of offshore environmental protection, pollution treatment of pesticide residues in an aquaculture environment and food safety guarantee.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a degrading bacterium for degrading pyrethroid pesticides in a seawater environment, a separation and purification method and application thereof. Background technique [0002] Pyrethroid pesticides are a class of high-efficiency, broad-spectrum pesticides with low toxicity, rapid excretion, and biodegradability. Therefore, they are often used in fishery production to kill parasites and harmful organisms. However, due to the lack of knowledge of scientific theory by farming users, a large number of pyrethroid pesticides are used in clearing ponds. At the same time, after being used in farmland, pyrethroid pesticides enter the offshore aquaculture area with the surface runoff; eventually leading to the deterioration of the water environment in the offshore sea area. The environmental quality in the sediment deteriorates, resulting in pesticide residue pollution. D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/01C02F101/38A62D101/04A62D101/28
Inventor 史西志刘菁华孙爱丽李德祥周磊陈炯
Owner 路亚科消防车辆制造有限公司
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