Micro fluidic chip apparatus by integrating continuous flow PCR and capillary electrophoresis function

Abstract

The invention discloses a micro fluidic chip apparatus by integrating a continuous flow PCR and a capillary electrophoresis function, which comprises a continuous flow type PCR chip and a capillary electrophoresis chip, and is characterized in that an output channel of the continuous flow type PCR chip is directly connected in a sample pool of the capillary electrophoresis chip, a sample introduction channel and a separating channel are provided on the capillary electrophoresis chip, the sample introduction channel and the separating channel present an intersect shape, the top of the sample introduction channel is the sample pool and a sample waste liquid pool, and the top of the separating channel is a buffer liquid pool and a waste liquid pool. The apparatus provided by the invention can enhance the automation degree and whole process operation speed for DNA detection, the operation step is reduced, and the apparatus is convenient for miniaturization and portable performance of the apparatus.

Description

technical field [0001] The invention belongs to the technical field of molecular biological detection, and in particular relates to a microfluidic chip device integrating the functions of continuous flow PCR and capillary electrophoresis. Background technique [0002] Polymerase chain reaction (PCR), also known as cell-free molecular cloning or specific DNA sequence in vitro primer-directed enzymatic amplification technology, is a rapid in vitro DNA amplification technology, as a main means of amplification and replication, It has been applied in a wide range of fields such as medical diagnosis, gene analysis, and forensic identification, and has gradually become one of the core technologies for nucleic acid analysis and amplification, and has had a profound impact on gene-related research. [0003] PCR technology manipulates the replication of DNA molecules by regulating the activity of polymerase at certain sites in the DNA. This reaction has the outstanding advantages of ...

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Problems solved by technology

[0023] The purpose of the present invention is to provide a microfluidic chip device integrating continuous flow PCR and capillary electrophoresis functions, which solves the problem of continuous flow PCR amplification and capillary electrophoresis instrument analysis in the prior art, which consumes more reagent and instrument costs for DNA detection. Conducive to issues such as instrument miniaturization and portability

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