Functional strain, and microbial agent and application thereof
A strain and functional technology, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problems of general deodorization effect, human injury, and olfactory failure, and achieve significant deodorization and antibacterial effects
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Embodiment 1
[0026] Embodiment one, the preparation of microbial bacterial agent:
[0027] 1. Strain activation: Inoculate the Bacillus subtilis strain W58 stored at -20°C on LB plate medium, and culture at 30°C for 22 hours to obtain activated functional strains;
[0028] 2. Preparation of seed liquid: Pick a single colony and insert the activated functional strain into LB liquid medium, shake and culture at 28°C for 24 hours to obtain seed liquid;
[0029] 3. Fermentation culture: the seed solution obtained in step (2) is inserted into the fermentation culture medium according to the volume percentage of 4%, and then cultivated for 26 hours at 30°C and a shaker speed of 170rpm, to obtain the active ingredient of subtilis subtilis Liquid microbial inoculant of Bacillus strain W58. The composition of the fermentation medium is: K 2 HPO 4 0.6%, KH 2 PO 4 0.1%, NaCl0.3%, MgSO 4 ·7H 2 O0.02%, CaCl 2 2H 2 O0.025%, (NH 4 ) 2 SO4 0.2%, 4% sucrose, 3% soybean powder, and the balance i...
Embodiment 2
[0030] Embodiment two, the preparation of microbial bacterial agent:
[0031] 1. Strain activation: Inoculate the Bacillus subtilis strain W58 stored at -20°C on LB plate medium, and culture it at 28°C for 24 hours to obtain activated functional strains;
[0032] 2. Preparation of seed solution: pick a single colony and insert activated functional strain into LB liquid medium, shake and culture at 32°C for 20 hours to obtain seed solution;
[0033] 3. Fermentation culture: the seed solution obtained in step (2) is inserted into the fermentation culture medium according to the volume percentage of 8%, and then cultivated for 28 hours at 28°C and a shaker speed of 190rpm, and the active ingredient is Subtilis subtilis Liquid microbial inoculant of Bacillus strain W58. The composition of the fermentation medium is: K 2 HPO 4 0.4%, KH 2 PO 4 0.3%, NaCl0.2%, MgSO 4 ·7H 2 O0.04%, CaCl 2 2H 2 O0.020%, (NH 4 ) 2 SO 4 0.3%, 3% sucrose, 1% soybean powder, and the balance is ...
Embodiment 3
[0034] Embodiment three: test
[0035] To a pile of rubbish that produces stench, the liquid microbial bacterial agent (diluted 10 times with tap water before use) of embodiment one gained is sprayed with sprayer, make preparation spraying range throughout the rubbish dumping area as far as possible. Sampling was carried out at different locations around the garbage, and H was measured 20 minutes before and after spraying the preparation. 2 S and NH 3 Concentration, the sampling point is 1m away from the edge of the odor source, the sampling height is 1m, 5 points are sampled, and the average value is taken. Odor concentration adopts three-point comparative smelly bag method (GB / T14675); ammonia adopts sodium hypochlorite-salicylic acid spectrophotometer (GB / T 14679); hydrogen sulfide adopts polyvinyl alcohol ammonium phosphate absorption-methylene blue colorimetric method (GB / T 16489). The results obtained are shown in Table 2.
[0036] Table 2
[0037] Test it...
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