Symbiotic and epiphytic aspergillus terreus of sponge and use for preparation of (+)- terrein

Abstract

The invention discloses symbiotic and epiphytic aspergillus terreus of sponge and use of aspergillus terreus for preparation of (+)- terrain. The symbiotic and epiphytic aspergillus terreus bacterial strain of the sponge is aspergillus terreus PF26CGMCC NO.5208. The step of symbiotic and epiphytic aspergillus terreus bacterial strain of the sponge for the preparation of (+)- terrain comprises a step of fermenting symbiotic and epiphytic aspergillus terreus bacterial strain of the sponge. The method concretely comprises the steps of sterilizing fermentation medium for 30 minutes at 115 DEG C; inoculating spores of aspergillus terreus PF26 into sterilized fermentation medium, wherein the inoculation amount is 4*10<6> pore / mL, and cultivating for 24 days in a shakerat 180rpm at 28 DEG C. The symbiotic and epiphytic aspergillus terreus disclosed by the invention is separated from phakellia fusca on the yongxing island on the South China Sea, and the yield of the (+)- terrain is greatly improved in comparison with the other bacterial strains at present and can be up to 3.71g / L.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a kind of aspergillus terreus and the preparation of (+)-terrein. Background technique [0002] Aspergillus terreus has been widely used in the production of lipid-lowering drug lovastatin and chemical raw material itaconic acid, but has not been used in the production of (+)-terrein. (+)-terrein is a cyclopentenone compound with a structure such as figure 1 As shown, it was originally isolated from the fermentation broth of Aspergillus terreus. In recent years, it has been found that (+)-terrein has very diverse activities. Secretion, inhibition of melanin synthesis, inhibition of skin keratin hyperplasia, inhibition of inflammatory response of dental pulp cells, promotion of fusion of osteoblasts and titanium scaffolds, etc. It can be predicted that (+)-terrein will have a good application prospect in many fields such as medical treatment, beauty treatment ...

AI Technical Summary

Problems solved by technology

[0003] The technology of preparing (+)-terrein by chemical synthesis often has a low overall yield, and requires extreme reaction conditions such as high temperature and high pressure, requires expensive reagents, or the final product does not have the selectivity of the three-dimensional structure

At present, the production of (+)-terrein prepared by microbial fermentation is very limited, ranging from hundreds of μg / L (Phattanawasin P, et al. Weed growth inhibitors from Aspergillus fischeri TISTR 3272. Natural Product Research 2007; 21: 1286-1291 ), as many as hundreds of mg / L (M.Demasi, et al.Studies on (+)-terrein as a New Class of Proteasome Inhibitors.Journal of the Brazilian Chemical Society 2010; 21:229-305), but failed Reach the g / L level with production value

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