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Detection kit for apolipoprotein E and preparation method thereof

A detection kit, apolipoprotein technology, applied in the field of biomedicine, can solve the problems of unsuitability for routine inspection, cumbersome operation, long time-consuming and the like

Inactive Publication Date: 2012-12-12
乐普(北京)诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of cumbersome operation, long time consumption, excessive waste of resources, and are not suitable for routine inspection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The kit of the present invention is exemplified as a double reagent, wherein:

[0034] Reagent R1:

[0035] Tris buffer 200mmol / L

[0036] Polyethylene glycol 8 mmol / L

[0037] Sodium azide 5 mmol / L

[0038] Sodium edetate 5mmol / L

[0039] Bovine serum albumin 5mmol / L;

[0040] Reagent R2:

[0041] Tris buffer 200mmol / L

[0042] Latex particles conjugated to apolipoprotein E monoclonal antibody 5% (v / v)

[0043] Tween-20 5mmol / L

[0044] Sodium azide 3 mmol / L

[0045] Sodium edetate 5mmol / L

[0046] Bovine serum albumin 5mmol / L.

Embodiment 2

[0047] Embodiment 2: Kit use method

[0048] 1. Reagent preparation, the reagent is a liquid double reagent, ready to use after opening the bottle, of which:

[0049] Reagent R1:

[0050] Tris buffer 200mmol / L

[0051] Polyethylene glycol 8 mmol / L

[0052] Sodium azide 5 mmol / L

[0053] Sodium edetate 5mmol / L

[0054] Bovine serum albumin 5mmol / L;

[0055] Reagent R2:

[0056] Tris buffer 200mmol / L

[0057] Latex particles conjugated to apolipoprotein E monoclonal antibody 5% (v / v)

[0058] Tween-20 5mmol / L

[0059] Sodium azide 3 mmol / L

[0060] Sodium edetate 5mmol / L

[0061] Bovine serum albumin 5mmol / L.

[0062] 2. Automatic biochemical analyzer parameter setting

[0063] (a) Detection temperature: 37°C

[0064] (b) Detection wavelength: the main wavelength is 340nm;

[0065] (c) Reaction time: 10 minutes.

[0066] 3. Detection steps (all completed in the automatic biochemical analyzer)

[0067] (a) Take 180 μL reagent 1 and 3 μL serum samples and mix well;...

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Abstract

A detection kit for apolipoprotein E. The kit body comprises: a reagent R1 containing a buffer solution, polyethylene glycol, sodium azide, sodium EDTA and bovine serum albumin; and a reagent R2 containing a buffer solution, latex particles with apolipoprotein E monoclonal antibodies, Twain-20, sodium azide, sodium EDTA and bovine serum albumin. A sample and the reagents are mixed in a certain volume ratio to perform a series of reactions; and then the reactants are placed in a half / full automatic biochemical analyzer for detection of absorbency change speed at a dominant wavelength of 340 nm, so as to calculate the concentration of apolipoprotein E. The kit has the advantages of accuracy, stability and convenience.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a detection kit for detecting human apolipoprotein E. Background technique [0002] Apolipoprotein E (apolipoprotein E, ApoE) is a polymorphic protein that participates in the transformation and metabolism of lipoproteins. Its gene can regulate many biological functions. The research on ApoE and its gene polymorphism is the current medical research. one of the hotspots. [0003] There is racial variation in ApoE polymorphism in the population, and the ApoE genotypes are highly different in different populations. The distribution of the ε4 allele frequency in Europeans showed a decreasing trend from north to south. The frequency of ε4 is low in Asians, in contrast to the high frequency of ε4 in Africans and Papua and New Guinea. [0004] Many evidences believe that ApoE polymorphism is the main cause of individual differences in the early stage and development of atheroscle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577
Inventor 谢兵
Owner 乐普(北京)诊断技术股份有限公司
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