Method for extracting and separating tannins components of immature fruits of medicine terminalia
A separation method and technology of tannins are applied in the directions of plant/algae/fungus/moss components, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve the problems of time-consuming, large consumption of solvents, troublesome operation, etc. Achieve high efficiency, high tannin content and low consumption
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Embodiment 1
[0016] Get 450g of green fruit medicinal material, pulverize, add acetone aqueous solution, soak and extract continuously three times at room temperature, add about 1.25L of acetone solution of 5 times of volume (v:v) for the first time and extract for 48 hours; Add 1 L of 80% acetone solution to extract each time for 24 hours, combine the extraction liquids, filter, and concentrate the filtrate under reduced pressure at 45°C to obtain an extract. Then extract with ethyl acetate, add 300ml of ethyl acetate each time, extract 5 times, combine the ethyl acetate extracts, concentrate under reduced pressure at 40°C, and concentrate until there is no ethyl acetate odor. Completely redissolve, apply Diaion HP-20 macroporous adsorption resin (the resin is first activated), and use 20%, 40% and 60% (v:v) methanol aqueous solution to elute successively, collect 60% methanol aqueous solution eluate, The obtained purified liquid was concentrated under reduced pressure at 40° C., then pla...
Embodiment 2
[0018] Take 500g of the green fruit medicinal material, grind it into fine powder, add 1L of 85% acetone aqueous solution three times in a row, extract for 24 hours each time, combine the extracts, filter, and concentrate the filtrate at 40°C under reduced pressure to obtain an extract. Then extract with ethyl acetate, 250ml each time, extract 5 times, combine the ethyl acetate extracts, concentrate under reduced pressure at 40°C until there is no ethyl acetate odor, redissolve the concentrate in 100ml of methanol, and adsorb on SEPABEDS-SP825 macropores Resin (resin activated first), eluted with 30%, 50%, and 70% (v; v) methanol aqueous solution in turn, collected 50% methanol aqueous solution eluate to obtain a purified solution, concentrated under reduced pressure at 40 ° C, and then placed In a constant temperature and reduced pressure drying oven, dry under reduced pressure to obtain 200 g of the tannin extract of the green fruit with a tannin content of 89.6%.
Embodiment 3
[0019] Embodiment three: prepare the capsule of green fruit tannin by technical method known in the art
[0020] Get 100g of the tannin extract of the green fruit of Example 1, use starch and dextrin as fillers, granulate with 3% starch slurry as a binding agent, add an appropriate amount of magnesium stearate, fill capsules, and each capsule contains Green fruit tannin 100mg, ready to pack.
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