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Basic orange II molecular imprinting solid-phase extraction filler, solid-phase extraction column and preparation methods and application thereof

A solid-phase extraction column and molecular imprinting technology, applied in chemical instruments and methods, and other chemical processes, can solve the problem of low separation selectivity

Inactive Publication Date: 2014-11-05
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The packing materials of conventional solid phase extraction columns mainly include bonded silica gel solid phase extraction materials, inorganic matrix solid phase extraction materials and organic polymer solid phase extraction materials, and their separation selectivity is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of Basic Orange II Molecularly Imprinted SPE Cartridge

[0028] (1) Preparation of molecularly imprinted polymer particles containing basic orange II with a tricyclic phenanthrene skeleton

[0029] Weigh 0.2mmol of Basic Orange II, add an appropriate amount of ethanol to make it just dissolve, then add 4mmol of acrylamide monomer, 6mmol of cross-linking agent maleic rosin glycol acrylate, 0.25g of azobisisobutyronitrile, 2mL Soybean oil, 10mL ethyl acetate, ultrasonic 20min, form a uniform organic phase solution A; solution B is an aqueous solution of sodium lauryl sulfate with a mass concentration of 0.10%; take 100mL of solution B and place it in a three-necked flask, and pour solution A into the solution In B, add a stirrer, condenser and thermometer, stir at a constant speed (210r / min) at room temperature for 20 minutes; heat the reaction system to slowly raise the temperature to 75°C, and react for 1h; connect the water separator, and slowly raise th...

Embodiment 2

[0041] 1. Preparation of Basic Orange II Molecularly Imprinted SPE Cartridge

[0042] (1) Preparation of molecularly imprinted polymer particles containing basic orange II with a tricyclic phenanthrene skeleton

[0043] Weigh 1mmol of Basic Orange II, add an appropriate amount of ethanol to make it just dissolve, then add 20mmol of acrylamide monomer, 30mmol of cross-linking agent maleic rosin glycol acrylate, 0.5g of azobisisobutyronitrile, 5mL of Soybean oil, 20mL ethyl acetate, ultrasonic 20min, form a uniform organic phase solution A; solution B is an aqueous solution of sodium lauryl sulfate with a mass concentration of 0.10%; take 300mL of solution B and place it in a three-necked flask, and pour solution A into solution B Add a stirrer, a condenser and a thermometer, and stir at a constant speed (230r / min) for 20 minutes at room temperature; heat the reaction system to slowly raise the temperature to 75°C, and react for 1h; connect the water separator, slowly raise the ...

Embodiment 3

[0055] 1. Preparation of Basic Orange II Molecularly Imprinted SPE Cartridge

[0056] (1) Preparation of molecularly imprinted polymer particles containing basic orange II with a tricyclic phenanthrene skeleton

[0057] Weigh 3mmol of Basic Orange II, add an appropriate amount of ethanol to make it just dissolve, then add 60mmol of acrylamide monomer, 90mmol of cross-linking agent maleic rosin glycol acrylate, 1.2g of azobisisobutyronitrile, 10mL of Soybean oil, 40mL ethyl acetate, ultrasonic 20min, form a uniform organic phase solution A; solution B is an aqueous solution of sodium lauryl sulfate with a mass concentration of 0.10%; take 600mL of solution B and place it in a three-necked flask, and pour solution A into solution B Add a stirrer, a condenser and a thermometer, and stir at a constant speed (250r / min) for 20 minutes at room temperature; heat to slowly raise the temperature of the reaction system to 75°C, and react for 1h; connect the water separator, and slowly he...

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Abstract

The invention discloses a basic orange II molecular imprinting solid-phase extraction filler, which is molecular imprinting polymer micro-particles which are prepared by taking basic orange II as template molecules, acrylamide as functional monomer, maleated rosin ethylene glycol acrylate as a cross-linking agent and azo-bis-iso-butyrynitrile as an initiator. The solid-phase extraction filler has a memory function to the stereo structure of basic orange II molecules, can realize the specificity, selective separation and enrichment of the basic orange II, is packed into the solid extraction column, can be used for detecting the selective separation, purification and enrichment of the basic orange II in sample extraction solution containing the basic orange II in foods, realizes high-sensitivity and high-selectivity detection for food samples containing the basic orange II, has stable recovery rate and small relative standard deviation and has the characteristics of simpler, quicker and more efficient operation and the like.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a basic orange II molecularly imprinted solid-phase extraction filler, a solid-phase extraction column and a preparation method and application thereof. Background technique [0002] Basic Orange Ⅱ (Basic Orange Ⅱ), the chemical name is 2,4-diamino azobenzene, the common name is "Wang Jinhuang", the chemical formula is C 12 h 12 N 4 ·HCl, with a molecular weight of 248.5, is obtained by coupling m-phenylenediamine after diazotization of aniline. It is an azo basic industrial dye. Basic orange is reddish-brown crystalline powder or brown blocky crystal with green luster, mainly used for dyeing leather products, textiles and wood products. According to investigations, it has high carcinogenicity, mutagenicity and other hazards, and China, the United States and the European Union have banned it from being used as a food additive. [0003] In the detection of food r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/285B01J20/26B01J20/30C08F220/06C08F2/44C08J9/28
Inventor 李小燕李梅雷福厚李鹏飞
Owner GUANGXI UNIV FOR NATITIES
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