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Method for extracting pomegranate peel polyphenols from pomegranate peel

A technology of pomegranate peels and crushed pomegranate peels, which can be used in skin care preparations, skin diseases, pharmaceutical formulations, etc., and can solve the problems that have not yet been reported about water as an extraction medium.

Inactive Publication Date: 2012-10-31
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the above-mentioned preparation methods use organic solvents as the extraction medium, and there are no relevant reports using water as the extraction medium.

Method used

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  • Method for extracting pomegranate peel polyphenols from pomegranate peel
  • Method for extracting pomegranate peel polyphenols from pomegranate peel
  • Method for extracting pomegranate peel polyphenols from pomegranate peel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1 prepares pomegranate peel polyphenol

[0070] 1. Crushing and sieving

[0071] Pomegranate peel (purchased from Chengdu Traditional Chinese Medicine Beverage Co., Ltd.), dried, crushed, sieved, 80-100 mesh.

[0072] 2. Crude polyphenol extraction

[0073] A microwave extractor (Shanghai Xinyi Microwave Chemical Technology Co., Ltd.) was used for auxiliary extraction. The optimized extraction parameters were: extraction temperature 75°C, liquid-solid ratio 30:1, extraction time 140S, and polyphenols were extracted with pomegranate peel powder of 80-100 mesh The total polyphenol content in the crude extract is 22.06%, and after vacuum concentration and vacuum freeze-drying, the total polyphenol content is 49.31%.

[0074] 3. Separation and purification

[0075] Using D101 macroporous resin, the speed is 2BV / h, the concentration into the column is 10mg / mL, the cleaning water is 5BV, and the eluent of 70% ethanol is 5.5BV to obtain pomegranate peel polypheno...

Embodiment 2

[0076] Example 2 Analysis of the pomegranate peel polyphenol active ingredient obtained in the present invention

[0077] The main active ingredients in the pomegranate peel polyphenols obtained in the present invention are punicalagin, ellagic acid, chlorogenic acid, epicatechin and quercetin, which are analyzed by RC-HPLC.

[0078] Chromatographic conditions for punicalagin: the chromatographic column is Hypersil ODS2 (250nm×4.6nm, 5μm Dalian Elite Company), the mobile phase is methanol-2% glacial acetic acid (7:93), the volume flow rate is 1.0mL / min, and the detection wavelength is 232nm, column temperature 25°C, theoretical plate number greater than 8000 based on ellagic acid, and resolution greater than 1.5.

[0079] Ellagic acid, chlorogenic acid, epicatechin and quercetin mixed standard chromatographic conditions: the chromatographic column is Hypersil ODS2 (250nm×4.6nm, 5μm Dalian Elite Company), and the mobile phase is acetonitrile-0.4% phosphoric acid (17: 83), the ...

Embodiment 3

[0081] Example 3 In vitro antioxidant activity assay

[0082] DPPH·,·OH,·O 2 - And the determination method of lipid peroxide:

[0083] 1. The scavenging activity of DPPH

[0084] Accurately weigh 20 mg of DPPH standard substance, and dilute to 500 mL with absolute ethanol to obtain a solution with a concentration of 0.04 mg / mL. Take 3mL of this solution, add 1mL of different concentrations of pomegranate peel extract, room temperature for 30min, measure the absorbance value, replace the sample with 1mL absolute ethanol in the blank group, and calculate the clearance rate according to the following formula:

[0085] DPPH clearance rate = (1-A 试样 / A 空白 )×100%

[0086] 2. OH ​​scavenging activity

[0087] Ferrous ions are used to catalyze hydrogen peroxide to generate hydroxyl radicals (Fonton reaction).

[0088] Take 1mL of 0.75mmol / L o-phenanthroline solution, 2mL of PBS solution and 1mL of distilled water, mix thoroughly, add 1mL of 0.75mmol / L ferrous sulfate, mix wel...

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Abstract

The invention belongs to the field of extraction from plants and particularly relates to a method for extracting pomegranate peel polyphenols from pomegranate peel. The method has the advantages of simplicity and convenience in operation, and high extraction efficiency. Particularly, the method comprises the steps of taking water as a solvent, extracting with microwaves to obtain coarse extract of pomegranate peel polyphenols, separating with macroporous resin, concentrating under reduced pressure, and vacuum freeze-drying to obtain the pomegranate peel polyphenols. The obtained coarse extract of pomegranate peel polyphenols and pomegranate peel polyphenols are higher in the total content of polyphenols, and the yield of the coarse extract reaches 40-50%, so that a new choice is provided for obtaining of pomegranate peel polyphenols.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to a method for extracting pomegranate peel polyphenols from pomegranate peel. Background technique [0002] Pomegranate Peel is the dried peel of pomegranate. It is sour in nature and astringent in taste. It has the effects of stopping bleeding, astringent intestines and killing insects. Pomegranate peel polyphenols is a general term for a class of polyphenolic hydroxyl compounds, including gallic tannins, ellagitannins, ellagic acid, chlorogenic acid, gallic acid, catechins, epicatechins, anthocyanins, ferulic A variety of compounds, such as acid and quercetin, mainly exist in the pomegranate peel, and the content is about 10-20% of its dry weight. [0003] In recent years, a large number of studies have shown that pomegranate peel polyphenols have many functions such as anti-free radicals, anti-mutagenesis, anti-aging, antibacterial, prevention of cardiovascular disea...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K8/97A61P17/18A61P39/06A61Q19/00A61K8/9789
Inventor 焦士蓉唐鹏程唐远谋冯慧刘佳
Owner XIHUA UNIV
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