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Establishment method for polygonum perfoliatum medicinal material finger print, and standard finger print thereof

A standard fingerprint and fingerprint technology, which is applied in the establishment of fingerprints and standard fingerprints of the herbal medicine, can solve the problems of lack of comprehensiveness and can not truly reflect the integrity of the efficacy of the medicinal herbs of the root, and achieve the goal of reflection. Complete information, avoiding the large absorption of near-ultraviolet impurity peaks, and good reproducibility

Inactive Publication Date: 2012-10-17
贵州远程制药有限责任公司 +1
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Problems solved by technology

Therefore, it is not comprehensive to test one or several active ingredients, and it cannot truly reflect the integrity of the efficacy of the drug.

Method used

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  • Establishment method for polygonum perfoliatum medicinal material finger print, and standard finger print thereof
  • Establishment method for polygonum perfoliatum medicinal material finger print, and standard finger print thereof
  • Establishment method for polygonum perfoliatum medicinal material finger print, and standard finger print thereof

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Embodiment Construction

[0044] Embodiments of the present invention: the establishment method of the HPLC fingerprint of the Chinese herbal medicine and the standard fingerprint of the Chinese medicinal material

[0045] 1. Instruments and reagents

[0046] 1.1 Instrument

[0047] Agilent 1100 high performance liquid chromatography; Agilent DAD detector; Agilent chromatography workstation.

[0048] 1.2 Reagent

[0049] Quercetin, quercetin-3-O-β-D-glucuronic acid, isolated and purified by laboratory members, identified as quercetin, quercetin- 3-O-β-D-glucuronic acid; acetonitrile, chromatographically pure; Robust purified water; syringe-type microporous membrane filter, diameter 13mm, 0.45μm;

[0050] 2. Methods and Results

[0051] 2.1 Selection of extraction method

[0052] In the course of the experiment, two methods of heating reflux and ultrasonic extraction were selected for determination, see attached figure 1 , figure 2 , and the results show that more chemical components can be refl...

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Abstract

The present invention discloses an establishment method for a polygonum perfoliatum medicinal material finger print, and a standard polygonum perfoliatum medicinal material finger print obtained by the method, wherein the method comprises the following steps: preparing a specimen solution, preparing a reference solution, measuring by a high performance liquid chromatograph, and processing data and map. Compared with the method in the prior art, the method of the present invention has the following advantages that: the method has characteristics of simpleness, stability, high precision, and good reproducibility; the obtained polygonum perfoliatum finger print has characteristics of more peaks, good peak shape, good peak separation effect, large peak area, easy identification, high similarity, accuracy and reliability, and can be adopted as the standard polygonum perfoliatum medicinal material finger print; a scientific approach capable of comprehensively evaluating the polygonum perfoliatum medicinal material is provided, the quality of the polygonum perfoliatum medicinal material can be effectively characterized, and the finger print can be adopted as one of standard indicators of quality detection and control of the polygonum perfoliatum medicinal material, and provides contributions for increase of qualities and therapeutic effects of polygonum perfoliatum and historical preparations thereof.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing high-performance liquid chromatography (HPLC) fingerprints of Chinese medicinal materials, and the standard fingerprints of medicinal materials obtained by the method. Background technique [0002] Most of the traditional Chinese medicines and their preparations in my country lack strict quality standards and scientific testing methods, so it is difficult to effectively control their internal quality, cannot guarantee the safety and effectiveness of medication, and do not meet the requirements of the international pharmaceutical market, which seriously restricts the Chinese medicine industry in my country. development of. Strengthening the research on the quality control of traditional Chinese medicinal materials is a key issue in the standardization and standardization of traditional Chinese medicine. O...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 周欣陈华国赵超田莉
Owner 贵州远程制药有限责任公司
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