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Bacillus subtilis RB and application thereof

A technology of Bacillus subtilis and enzyme bacteria, applied in the application field of Bacillus subtilis RB, can solve the problems of low utilization rate of cellulose, hemicellulose and protein, poor adaptability of enzymes, inability to synthesize amino acids, etc., and achieve adaptability The effect of strong, increased output and strong processing capacity

Inactive Publication Date: 2012-10-17
CHONGQING RUIBAO AGRI IND GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, enzyme bacteria are widely used in the treatment of poultry manure or crop straw, but the commercially available enzymes have poor adaptability and low utilization rate of cellulose, hemicellulose and protein in poultry manure or crop straw
Publication number is that the Chinese patent of CN101215532A discloses a kind of Bacillus megaterium M1, can improve the adaptability of enzyme bacteria to a certain extent after adding commercially available enzyme bacteria, but can not utilize poultry excrement or crops after adding Bacillus megaterium M1 Amino Acid Synthesis from Raw Materials in Manuscript

Method used

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  • Bacillus subtilis RB and application thereof
  • Bacillus subtilis RB and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, bacillus subtilis ( Bacillus subtilis ) separation of RB

[0017] Mix 10g of beneficial microorganisms isolated and screened from nature evenly with the nutrient base, which contains 5-10g of cornstarch, 5-10g of brown sugar and 10-20g of wheat bran, pH 6.0-7.0, humidity 30-35%, and then Add 0.005-0.05 g of hydroxylamine or ethyl imine; at a temperature of 25-35° C. with a temperature difference of 8-10° C., cultivate for 2-3 days, and use an inoculation loop to screen out Bacillus subtilis RB. The nutrient base may also include 3-5 g of fish meal, 3-5 g of vegetable cake or 35-50 g of polished rice bran.

Embodiment 2

[0018] Embodiment 2, the cultivation of Bacillus subtilis RB

[0019] Bacillus subtilis RB medium: sucrose 50g, (NH 4 ) 2 SO 4 6g, K 2 SO 4 2.5g, CaSO 4 2.5g, agar powder 15g and distilled water 1000mL.

[0020] The culture conditions are as follows: the temperature is 30° C., and the pH is 6.0.

Embodiment 3

[0021] Embodiment 3, the application of Bacillus subtilis RB

[0022] The enzyme bacteria of the present invention is obtained by mixing Bacillus subtilis RB with commercially available enzyme bacteria in a weight ratio of 1-2:5, that is, the mass percentage of Bacillus subtilis RB in the enzyme bacteria is 20%-40%.

[0023] Mix and stir 1kg of enzyme bacteria with 100kg of sludge, 100kg of poultry manure or 100kg of crop straw, and carry out open aerobic solid-state fermentation at a temperature of 60°C for 7 days, and then enter the process of drying, crushing, and manufacturing through a conveying system. Grain and sieve to obtain amino acid fertilizer. Exhaust gases from the drying step can be pumped into a tank and returned to the fermentation step.

[0024] 1 kg of ferment bacteria was mixed with 100 kg of sludge, 100 kg of poultry manure or 100 kg of crop stalks, and fermented in the same way as above. As a control test, the results are shown in Table 1.

[0025] Th...

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Abstract

The present invention discloses Bacillus subtilis RB, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), wherein the preservation number is CGMCGNO.6268. The strain provides high utilization rates for cellulose, hemicellulose and protein in livestock manure or crop straws, has strong sludge treatment capacity and strong adaptability, and can be used for treatments of sludge and decomposition of livestock manure or crop straws, wherein the product has high amino acid content, and can be used as fertilizer so as to achieve utilization of waste into treasure and provide wide application prospects.

Description

technical field [0001] The present invention relates to a kind of bacterium, be specifically related to Bacillus subtilis ( Bacillus subtilis ) RB, also relates to the application of Bacillus subtilis RB. Background technique [0002] Enzyme bacteria is a beneficial microbial group composed of some aerobic microorganisms that can produce a large number of various hydrolytic enzymes, mainly composed of more than 20 strains of yeast, bacteria and filamentous bacteria. At present, enzyme bacteria are widely used in the treatment of poultry manure or crop straw, but the commercially available enzymes have poor adaptability and low utilization rate of cellulose, hemicellulose and protein in poultry manure or crop straw. Publication number is that the Chinese patent of CN101215532A discloses a kind of Bacillus megaterium M1, can improve the adaptability of enzyme bacteria to a certain extent after adding commercially available enzyme bacteria, but can not utilize poultry excremen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/125
CPCY02W30/40
Inventor 张怀忠
Owner CHONGQING RUIBAO AGRI IND GROUP
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