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Hantavirus glucoprotein specific T cell epitope peptide and application thereof

An epitope peptide and specific technology, applied in the field of Hantaan virus glycoprotein-specific CD4+T cell epitope peptide and CTL epitope peptide, can solve the problem of no specific prevention and treatment methods

Active Publication Date: 2013-11-27
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] HFRS caused by HTNV infection has high morbidity and mortality, but so far there is no specific prevention and treatment

Method used

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  • Hantavirus glucoprotein specific T cell epitope peptide and application thereof
  • Hantavirus glucoprotein specific T cell epitope peptide and application thereof
  • Hantavirus glucoprotein specific T cell epitope peptide and application thereof

Examples

Experimental program
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Effect test

Embodiment Construction

[0098] The applicant synthesized partially overlapping 15 peptides covering the full length of the Gn / Gc region of the HTNV 76-118 strain, and used solid-phase enzyme linked immunospot assay (ELISPOT) and magnetic bead separation technology to detect peripheral blood mononuclei of HFRS patients. Cells (peripheral blood mononuclear cell, PBMC), the system identified CD4 on Gn / Gc + 15 peptide epitopes and CD8-containing + 15 peptides of T cell epitopes.

[0099] In the following examples, the terms involved are defined as follows:

[0100]The HTNV-Gn / Gc 15 peptide starts from the N-terminus and sequentially synthesizes partially overlapping peptides. Each peptide is 15 amino acid residues in length, and two adjacent peptides overlap by 11 amino acid residues.

[0101] T cell epitope peptide was identified by detecting the level of interferon-gamma (Interferon-gamma, IFN-gamma) secreted by PBMC by ELISPOT.

[0102] CD4 + T cell epitope peptide or CTL epitope peptide is used t...

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Abstract

The invention discloses an HTNV-Gn / Gc specific T cell epitope peptide and an application thereof. The amino acid sequence of the HTNV-Gn / Gc specific T cell epitope peptide is one selected from SEQ ID NO. 1 to SEQ ID NO. 75. The HTNV-Gn / Gc specific T cell epitope peptide comprises CD4<+>T cell epitope and CTL epitope, and can respectively induce CD4<+>T cell and CD8<+>T cell to generate intensive cellular immunologic response and to secrete high-level IFN-gamma. The HTNV-Gn / Gc specific T cell epitope peptide can be used in the preparation of T cell epitope peptide vaccines, and can be used in inducing CD4<+>T cell or cytotoxicity T cell generating epitope peptide specificity. Therefore, the HTNV-Gn / Gc specific T cell epitope peptide has a good exploitation and application prospect in the field of HFRS specific immunotherapy.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of Hantaan virus, and relates to the specific CD4 of Hantaan virus glycoprotein + T cell epitope peptide and CTL epitope peptide and application thereof. Background technique [0002] Hantaan virus (HTNV) is an important virus among the pathogenic microorganisms within the scope of verification of the Protocol on the Prohibition of Biological Weapons. It belongs to the genus Hantavirus (HTV) of the Bunyaviridae family and is the prototype of HTV. HTV viruses are all single-stranded negative-sense RNA enveloped viruses, and the genome includes three segments: large (L), medium (M), and small (S), in which the L segment encodes RNA polymerase, and the M segment encodes envelope glycoprotein 1 and 2 (glycoprotein 1, G1 / Gn and glycoprotein 2, G2 / Gc), the S segment encodes nucleocapsid protein (NP). The HTNV 76-118 strain first isolated by Li Haowang et al. in 1978 is the representat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K39/12A61P31/14C12N5/0783C07K16/10
Inventor 马樱张赟张春梅庄然徐竹蔚易静刘蓓张宇丝陈丽华杨琨宋朝君李琦方亮周幸春刘志佳金伯泉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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