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Fermentation and refining method for sapindus mukurossi saponin extract

A technology of saponin and refining method, applied in the field of fermentation and refining of plant extracts, can solve the problems of difficult continuous operation of operation units, high product impurity content, poor product stability, etc., and achieves eliminating evaporation or drying units, The effect of stable product quality and reduced energy consumption

Active Publication Date: 2012-10-10
广州德谷个人护理用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The water extract of Sapindus chinensis is rich in saponin and carbohydrates, which need to be further separated and refined, and a large amount of foam will be generated during vacuum evaporation and concentration, which makes it difficult for the operation unit to be continuously operated; the water extract is directly used as a liquid detergent or pesticide synergist, because the water extract contains rich carbohydrates, resulting in poor product stability; the water extract directly spray-dried to prepare dry powder requires high energy consumption, and at the same time, the product has a high impurity content in the dry powder
[0007] Although the use of saponin in Sapindus fruit peel is known, the method of refining it to obtain a liquid product with excellent use effect is still a blank in the art

Method used

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  • Fermentation and refining method for sapindus mukurossi saponin extract
  • Fermentation and refining method for sapindus mukurossi saponin extract
  • Fermentation and refining method for sapindus mukurossi saponin extract

Examples

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Embodiment 1

[0038] Remove impurities and iron from the air-dried Sapinberry peel (moisture content ≤ 12%). Iron may be mixed in the collection process, which will have a negative impact on the crushing equipment. Use an electric pulverizer to crush the pericarp into fragments of 1 mm or less, add water 5 times its mass, and extract saponin by shaking in a constant temperature shaker at 60°C and 150 rpm for 4 hours. Place the extract in a centrifuge with a rotating speed of 3000rpm, centrifuge for 15min, and collect the supernatant, which is the aqueous extract of Sapindus chinensis. With 10% NaOH solution, add 1-2 drops dropwise to adjust the pH value of the solution to 4.8. Add 10 IU cellobiase to the solution, and carry out saccharification for 96 hours in a full-temperature shaking incubator at 45° C. and 180 rpm.

[0039] 30% NaOH solution was used to adjust the pH value of the solution to 7.0. Divide the solution into 4 parts, numbered 1,2,3,4. Add yeast extract medium to group 1,...

Embodiment 2

[0043] according to figure 1 The process is to remove impurities and iron from the air-dried sapindus fruit peel, crush the peel with an electric grinder, and crush it into fragments of 1mm or less, add water 7 times its mass, and oscillate in a constant temperature shaker at 50°C and 150 rpm Extract saponin, 5h. Place the extract in a centrifuge with a rotating speed of 3000rpm, centrifuge for 15min, and collect the supernatant, which is the aqueous extract of Sapindus chinensis. The concentration of sugar in the water extract was detected by high performance liquid chromatography ( figure 2 ). The pH of the solution was adjusted to 4.7 with 10% NaOH solution. Add 10 IU cellobiase and 20 IU mannanase to the solution, and carry out saccharification in a shaking incubator at 45° C. and 180 rpm for 96 hours. The sugar concentration in the enzymatic saccharification solution was detected by high performance liquid chromatography ( image 3 ). 30% NaOH solution was used to ...

Embodiment 3

[0046] Remove the impurity and iron from the air-dried Sapindus fruit peel, pulverize the peel with an electric grinder, pass through a 20-mesh sieve, add water 6 times its mass, and extract saponin by shaking in a constant temperature shaker at 70°C and 150 rpm for 2 hours. The extract was placed in a centrifuge with a rotating speed of 3000 rpm, centrifuged for 15 minutes, and the supernatant was collected, which was the aqueous extract of sapindus sapin. The pH of the solution was adjusted to 4.8 with 10% NaOH solution. Add 10 IU cellobiase, 20 IU mannanase and 0.2 mg / ml pectinase to the solution, and carry out saccharification for 80 h in a full-temperature shaking incubator at 50° C. and 180 rpm. 30% NaOH solution to adjust the pH of the solution to 7.5. Add the sterilized yeast extract medium to the enzymolysis solution (in terms of fermentation broth volume, MgSO 4 ·7H 2 O 0.4g / L, KH 2 PO 3 0.4g / L, NaCl0.09g / L, yeast extract 5g / L), lactic acid bacteria 0.4% (mass r...

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Abstract

The invention provides a fermentation and refining method for sapindus mukurossi saponin extract, which includes the following steps: sapindus mukurossi extract is enzymatically saccharified, and lactic acid bacteria are then used for inoculation and fermentation. The invention also provides a fermented and refined product of the sapindus mukurossi saponin extract. According to the characteristic of the sapindus mukurossi peel extract containing high-density cellobiose and mannose, in the fermentation and refining method, the sapindus mukurossi peel extract is enzymatically saccharified and fermented to convert carbohydrates and a small amount of protein in the sapindus mukurossi peel extract into lactic acid, consequently, sapindus mukurossi peels are sufficiently utilized, the compound product of sapindus mukurossi saponin and lactic acid is obtained, and the high-value utilization of sapindus mukurossi fruits is realized. According to the method, carbohydrates, protein and other interfering impurities in the aqueous sapindus mukurossi extract are directly converted into active components in the final product, the quality of the prepared refined sapindus mukurossi liquid product is stable, the purity of the refined sapindus mukurossi liquid product is high, and the refined sapindus mukurossi liquid product can be used in the preparation of liquid detergent or other liquid products.

Description

technical field [0001] The invention belongs to the field of steroid compounds, and in particular relates to a method for fermenting and refining plant extracts containing steroid compounds. Background technique [0002] Natural saponin, also known as natural saponin, is a general term for plant glycosides that can form aqueous or colloidal solutions and soapy foam. It is composed of saponin and sugar, uronic acid or other organic acids. According to the molecular structure of known saponins, saponins can be divided into two categories, one is steroidal saponins, and the other is triterpene saponins. Saponins are mostly white or milky white amorphous powder, a few are crystalline, bitter and pungent, and irritating to mucous membranes. Saponins are generally soluble in water, methanol and dilute ethanol, easily soluble in hot water, hot methanol and hot ethanol, insoluble in ether, chloroform and benzene. Saponins are strong surfactants that can form soaps even at high dil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C07J63/00C07H15/256C07H1/08
Inventor 蒋建新赵丹青唐勇卜令习朱莉伟张卫明孙达峰
Owner 广州德谷个人护理用品有限公司
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