White flowered Loropetalum chinense total phenol extractive and preparation method and application thereof
A technology for extracts and total phenols, which is applied in the application field of developing new hemostatic drugs, can solve problems such as unclear preparation methods, and achieve the effects of avoiding loss of target components, high extraction rate, and reducing daily dosage.
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Embodiment 1
[0018] Embodiment 1, content determination of medicinal active ingredient, condition optimization
[0019] (1) Determination of the content of total phenolic components in P.
[0020] Preparation of reference solution
[0021] Accurately weigh 10 mg of gallic acid powder, dissolve it in 60% ethanol and make it up to 100 mL to obtain a 0.1 mg·mL-1 reference substance solution.
[0022] Preparation of the test solution
[0023] Take the medicinal material of P. chinensis, crush it and pass it through a No. 3 sieve, weigh 1.0g of the powder, add 30mL of 60% ethanol for ultrasonic extraction for 30min, filter, and dilute the solution to 30mL, then dilute the solution 30 times with 60% ethanol, shake well, Instantly.
[0024] Choice of measurement wavelength
[0025] Accurately take appropriate amount of the test solution and the reference solution, add 1.0mL of Folin-phenol reagent, let it stand for 5-7 minutes, then add 1.0mL of 10% Na2CO3 solution, mix well and d...
Embodiment 2
[0057] Take 1000g of dried medicinal material of P. chinensis, cut off (2~5cm), use 5 times of 40% ethanol, heat and reflux extraction for 3 times, filter, combine to obtain extract A, concentrate under reduced pressure at 60°C to 0.1g (crude medicinal material) mL -1 , to obtain concentrate B. The concentrated solution B has been treated with macroporous adsorption resin HPD-300, followed by 4BV of deionized water, 4BV10%, 3BV30% and 2BV95% ethanol solution at 3mL min -1 The flow rate was eluted, and the 50% ethanol eluate was collected and vacuum-dried to obtain 20 g of total phenolic extracts of P. alba with a UV content of 55%. The results of the pharmacological activity test showed that compared with the blank group, the total phenolic extract group could significantly shorten the tail docking bleeding time of mice (P < 0.01), the shortening rate was 28.12%, and the shortening rate of Yunnan Baiyao was 48.92%; And the total phenolic extract group can significantly short...
Embodiment 3
[0060] Take 1000g of dried medicinal material of P. chinensis, cut off (2~5cm), use 5 times of 80% ethanol, heat and reflux extract 3 times, filter, combine to obtain extract A, concentrate under reduced pressure at 60°C to 0.3g (raw medicinal material) mL -1 , to obtain concentrate B. The concentrated solution B has been treated with macroporous adsorption resin HPD-450, followed by 4BV of deionized water, 4BV10%, 3BV50% and 2BV95% ethanol solution at 3mL min -1 The flow rate was eluted, and the 50% ethanol eluate was collected and vacuum-dried to obtain 15 g of total phenolic extracts of P. alba with a UV content of 60%. The results of the pharmacological activity test showed that compared with the blank group, the total phenolic extract group could significantly shorten the tail docking bleeding time of mice (P < 0.01), the shortening rate was 40.33%, and the shortening rate of Yunnan Baiyao was 48.92%; And the total phenolic extract group can significantly shorten the co...
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