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White flowered Loropetalum chinense total phenol extractive and preparation method and application thereof

A technology for extracts and total phenols, which is applied in the application field of developing new hemostatic drugs, can solve problems such as unclear preparation methods, and achieve the effects of avoiding loss of target components, high extraction rate, and reducing daily dosage.

Inactive Publication Date: 2012-10-10
JIANGXI HERBFINE HI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] On the basis of the good hemostatic activity of the medicinal material Aloropetalum japonicus, aiming at the current unclear problem of its pharmacologically active substances and preparation methods, the present invention mainly uses the total phenolic substances of the medicinal active ingredients as an investigation index to extract, extract, and Purification to obtain a total phenolic extract with a UV content greater than 50%

Method used

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  • White flowered Loropetalum chinense total phenol extractive and preparation method and application thereof
  • White flowered Loropetalum chinense total phenol extractive and preparation method and application thereof
  • White flowered Loropetalum chinense total phenol extractive and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, content determination of medicinal active ingredient, condition optimization

[0019] (1) Determination of the content of total phenolic components in P.

[0020] Preparation of reference solution

[0021] Accurately weigh 10 mg of gallic acid powder, dissolve it in 60% ethanol and make it up to 100 mL to obtain a 0.1 mg·mL-1 reference substance solution.

[0022] Preparation of the test solution

[0023] Take the medicinal material of P. chinensis, crush it and pass it through a No. 3 sieve, weigh 1.0g of the powder, add 30mL of 60% ethanol for ultrasonic extraction for 30min, filter, and dilute the solution to 30mL, then dilute the solution 30 times with 60% ethanol, shake well, Instantly.

[0024] Choice of measurement wavelength

[0025] Accurately take appropriate amount of the test solution and the reference solution, add 1.0mL of Folin-phenol reagent, let it stand for 5-7 minutes, then add 1.0mL of 10% Na2CO3 solution, mix well and d...

Embodiment 2

[0057] Take 1000g of dried medicinal material of P. chinensis, cut off (2~5cm), use 5 times of 40% ethanol, heat and reflux extraction for 3 times, filter, combine to obtain extract A, concentrate under reduced pressure at 60°C to 0.1g (crude medicinal material) mL -1 , to obtain concentrate B. The concentrated solution B has been treated with macroporous adsorption resin HPD-300, followed by 4BV of deionized water, 4BV10%, 3BV30% and 2BV95% ethanol solution at 3mL min -1 The flow rate was eluted, and the 50% ethanol eluate was collected and vacuum-dried to obtain 20 g of total phenolic extracts of P. alba with a UV content of 55%. The results of the pharmacological activity test showed that compared with the blank group, the total phenolic extract group could significantly shorten the tail docking bleeding time of mice (P < 0.01), the shortening rate was 28.12%, and the shortening rate of Yunnan Baiyao was 48.92%; And the total phenolic extract group can significantly short...

Embodiment 3

[0060] Take 1000g of dried medicinal material of P. chinensis, cut off (2~5cm), use 5 times of 80% ethanol, heat and reflux extract 3 times, filter, combine to obtain extract A, concentrate under reduced pressure at 60°C to 0.3g (raw medicinal material) mL -1 , to obtain concentrate B. The concentrated solution B has been treated with macroporous adsorption resin HPD-450, followed by 4BV of deionized water, 4BV10%, 3BV50% and 2BV95% ethanol solution at 3mL min -1 The flow rate was eluted, and the 50% ethanol eluate was collected and vacuum-dried to obtain 15 g of total phenolic extracts of P. alba with a UV content of 60%. The results of the pharmacological activity test showed that compared with the blank group, the total phenolic extract group could significantly shorten the tail docking bleeding time of mice (P < 0.01), the shortening rate was 40.33%, and the shortening rate of Yunnan Baiyao was 48.92%; And the total phenolic extract group can significantly shorten the co...

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Abstract

The invention relates to an extractive taking white flowered Loropetalum chinense total phenol as the main and a preparation method and application of the extractive. The preparation method comprises the following steps of performing reflux extraction on a white flowered Loropetalum chinense medicinal material through 8 times (medicinal material / solvent: g / L) of alcohol (60 percent) for three times for 2 hours each time; merging extracts and concentrating to remove the alcohol to obtain a merged and concentrated extract; then, adding macroreticular resin to the merged and concentrated extract; performing gradient elution in sequence by using water and alcohol; collecting eluents of which the alcohol concentrations are between 50 percent and 70 percent; merging; and performing vacuum drying to obtain the extractive, wherein the ultraviolet (UV) content of the total phenol extractive can reach up to at least 50 percent. The extractive mainly comprises the following components: gallate, rutin, myricetin, quercetin and kaempferol. Shown by pharmacology experiments, the extractive is obvious in stypticity.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and relates to a preparation process of total phenolic extracts of P. candidum and its application in developing new hemostatic drugs. Background technique [0002] White flowered loropetalum Loropetalum chinense (R. Brown) Oliv. It is a plant of the genus Roropetaceae in the family Hamamelis, which was first recorded in "Plant Names and Facts", and has the effects of "converging and stopping bleeding, astringent intestines and diarrhea, promoting muscle growth, anti-inflammatory, and analgesic". It is used to treat traumatic bleeding. It is also recorded in "Fujian Folk Herbal Medicine" that the leaves of Loropetalum have the functions of "relaxing tendons and activating blood circulation, relieving heat and stopping diarrhea. Treating abdominal pain, flashing tendons, watery diarrhea, calluses, and traumatic bleeding". As early as the 1970s, it was reported in the literature that t...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P7/04
Inventor 冯育林杨世林张武岗简晖罗晓健陈兰英饶毅范玫玫
Owner JIANGXI HERBFINE HI TECH
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