Blood leucocyte separating medium and preparation method and vacuum blood collection tube

Abstract

The invention discloses a blood leucocyte separating medium and application thereof, a preparation method of the blood leucocyte separating medium, and a blood leucocyte separating vacuum blood collection tube with the blood leucocyte separating medium and application thereof. The blood leucocyte separating medium comprises glucan T500, hydroxyethyl starch, meglumine diatrizoate, water, sodium citrate, NaN3, poly(diallyldimethylammonium chloride) and polyvinyl pyrrolidone, the proportion of parts by weight of which is 18.5-21.5:1.5-2.5:2-3:69:3.8:0.1:0.05-0.15:2-3. The invention has the advantages that clinical blood collection and anticoagulant leucocyte seperation can be completed at a time, the sodium citrate in the blood leucocyte separating medium and calcium ions in blood are chelate and anticoagulant, the technical problem of damaging the liquid level layer of the separating medium does not exit, a supernatant liquor is sucked up without needing centrifugation after natural precipitation, a complex cell seperation technology is transformed into a simple blood collection seperation process, the processes are easily united, the repeatability of test results is good, and the quality controllability is strong.

Description

technical field [0001] The invention relates to a vacuum container for blood collection and white blood cell density natural sedimentation separation and collection in the field of medical clinical testing, in particular to a blood leukocyte separation liquid, a method for making the blood leukocyte separation liquid, and a blood leukocyte separation vacuum blood collection tube with the blood leukocyte separation liquid. Background technique [0002] In today's medical clinical tests, blood virus gene tests and immune cell function tests all require the isolation of nucleated leukocytes or immune lymphocytes with the ability to phagocytize viruses after blood collection. Traditional cell separation methods generally use the density of various cells Different, prepare cell separation fluid with high ionic strength of the same density of various target cells, add quantitatively to the test tube, slowly add anticoagulant blood into the tube along the tube wall (cannot break thr...

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Problems solved by technology

[0002] In today's medical clinical tests, blood virus gene tests and immune cell function tests all require the isolation of nucleated leukocytes or immune lymphocytes with the ability to phagocytize viruses after blood collection. Traditional cell separation methods generally use the density of various cells Different, prepare cell separation fluid with high ionic strength of the same density of various target cells, add quantitatively to the test tube, slowly add anticoagulant blood into the tube along the tube wall (cannot break through the liquid surface layer of the separation fluid), and then use a centrifuge to centrifuge (The requirement for the range of centrifugal force is very narrow), the centrifugal force and centrifugal radius, and the actual speed of the centrifuge are only theoretically derived values, which cannot be measured and controlled in practice. The professionalism is very strong, the human factors are large, and the repeatability of the separation effect is poor.

[0003] Based on the many defects of the above-mentioned traditional cell separation methods: strong professionalism and difficult operation, there is no unified reagent and operating procedure, the collection rate of target cells is very low, time-consuming and labor-intensive, and repeated procedures are often required. collection

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