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Ultra-multiple-pore hydrogel for microbial fermentation and preparation method and application thereof

A technology of porous hydrogel and microbial fermentation, applied in the field of microbial fermentation of ultra-porous hydrogel and its preparation, can solve the problems of environmental hazards, cumbersome steps, limited large-scale production, etc., achieves less pollution, convenient operation, and has The effect of generality and universality

Inactive Publication Date: 2012-10-03
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the above, the porous gel prepared by the foaming method often adds chemical agents such as porogens and foaming agents, which also cause certain harm to the environment, and the steps are cumbersome, and large-scale production is limited.

Method used

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  • Ultra-multiple-pore hydrogel for microbial fermentation and preparation method and application thereof

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Experimental program
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Embodiment 1

[0031] 1) Preparation of gel pre-polymerization solution: Add 2.0g of acrylamide and 0.1g of N,N'-methylenebisacrylamide to 10mL of deionized water in turn, stir for 20min until all of them are dissolved, and then the gel pre-polymerization solution is obtained ;

[0032] 2) Rehydration and activation of yeast: Dissolve 0.8g of Angel high-activity dry yeast in 10mL of deionized water at 30°C, seal with plastic wrap, stir for 20 minutes, add 0.4g of sugar and continue stirring for 5 minutes to obtain an active yeast solution;

[0033] 3) Preparation of superporous gel: Add the active yeast solution to the gel prepolymerization solution at 30°C, stir for 20 minutes under sealed conditions, add 0.01 g of ammonium persulfate and 160 μL of tetramethylethylenediamine in sequence, and after 5 minutes Slowly pour the mixed solution into a watch glass with an inner diameter of 6cm, and react for 6h;

[0034] 4) Purification of superporous gel: Cut the reacted product into discs with a...

Embodiment 2

[0037] 1) Preparation of gel prepolymerization solution: add 1.0g of isopropylacrylamide and 0.1g of N,N'-methylenebisacrylamide into 10mL of deionized water in turn, stir for 15min until all are dissolved, and the gel is obtained Pre-polymerization liquid;

[0038] 2) Rehydration and activation of yeast: Dissolve 1.0g of Angel high-activity dry yeast in 5mL of deionized water at 26°C, seal with plastic wrap, stir for 25 minutes, add 0.4g of glucose and continue stirring for 10 minutes to obtain an active yeast solution;

[0039] 3) Preparation of superporous gel: Add the active yeast solution to the gel pre-polymerization solution at 26°C, stir for 15 minutes under sealed conditions, add 0.1g potassium persulfate and 80 μL tetramethylethylenediamine in sequence, and after 5 minutes Slowly pour the mixed solution into a watch glass with an inner diameter of 4cm, and react for 4h;

[0040] 4) Purification of superporous gel: cut the reacted product into discs with a diameter o...

Embodiment 3

[0043] 1) Preparation of gel prepolymerization solution: add 1.5g of 2-(dimethylamino)ethyl-methacrylamide and 0.15g of ethylene glycol di(meth)acrylate into 10mL of deionized water in turn, stir for 20min, until Dissolved completely, the gel pre-polymerization solution is obtained;

[0044] 2) Rehydration and activation of yeast: Dissolve 1.2g Champagne yeast in 12mL deionized water at 28°C, seal with plastic wrap, stir for 30min, add 0.6g carboxymethyl chitosan and continue stirring for 20min to obtain active yeast solution;

[0045] 3) Preparation of superporous gel: Add the active yeast solution to the gel pre-polymerization solution at 28°C, stir for 20 minutes under sealed conditions, add 0.05 g of potassium persulfate and 100 μL of tetramethylpropylenediamine in sequence, and after 5 minutes Slowly pour the mixed solution into a watch glass with an inner diameter of 8cm, and react for 4h;

[0046] 4) Purification of superporous gel: Cut the reacted product into discs ...

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Abstract

The invention relates to an ultra-multiple-pore hydrogel for microbial fermentation and a preparation method and application thereof. The preparation method includes: (1) preparing gel pre-polymerizing liquid; (2) rehydration activation of dried yeast; (3) preparation of ultra-multiple-pore gel; and (4) purification of the ultra-multiple-pore gel. The theory is that after rehydration activation of the dried yeast, fermentation reaction is conducted in the gel to generate carbon dioxide, so that the effect of the foaming agents is played to generate the ultra-multiple-pore gel. The ultra-multiple-pore hydrogel is combined with the fermentation technology of microorganisms, can achieve non-toxic and harmless production and production efficiency of the porous gel preparation process, has generality and universality and can be applied to the field of dye absorption, heavy metal ion enrichment and recovering, drug carriers and biomedicine support materials.

Description

technical field [0001] The invention relates to the fields of microorganisms, intelligent polymer materials, biomedicine and environmental protection, in particular to a microbial fermented superporous hydrogel and its preparation method and application. Background technique [0002] Hydrogels (hydrogels) are a kind of hydrophilic but water-insoluble polymers, which can quickly swell to an equilibrium volume in water and still maintain their shape and three-dimensional space network structure. It is a fast-growing functional polymer material that integrates, slow release and rapid development. Because of its unique water absorption, water retention and bionic properties, hydrogels are widely used in the fields of industry, agriculture, medicine and bioengineering materials. The swelling and deswelling time of traditional hydrogels can range from a few seconds to hours or even days. Introducing a porous structure into the gel, that is, preparing an ultra-porous hydrogel, can ...

Claims

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Application Information

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IPC IPC(8): C08J9/08C08J3/075C08F220/56C08F222/38C08F220/54C08F220/60C08F222/14C08F220/28C08F220/06
Inventor 张青松张婷婷陈怡婷陈莉闫明洋陈冰洁
Owner TIANJIN POLYTECHNIC UNIV
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