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Immunofluorescence test strip component for quickly quantitatively testing troponin-T and test card component manufactured by same and production process of immunofluorescence test strip

A technology for quantitative detection of troponin, applied in the field of medical testing, can solve the problems of long time, time-consuming and complicated process of continuous increase, and achieve the effects of sufficient reaction, increased dilution ratio and simple operation.

Active Publication Date: 2012-09-26
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(3) It lasts longer in the blood
[0007] Application No. 200610130403.3 discloses a method for detecting cardiac troponin T by chemical immunoassay, which mainly uses horseradish peroxide enzymatic chemiluminescence system, and adopts double monoclonal antibody sandwich method and / or competition method for detection. This method requires the sample The complex with the enzyme (solidified and solid-phase support) is placed in the reaction pool, and after washing, the enzyme substrate, oxidant and enhancer can be added to emit light. The process of this method is complicated and time-consuming.

Method used

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  • Immunofluorescence test strip component for quickly quantitatively testing troponin-T and test card component manufactured by same and production process of immunofluorescence test strip
  • Immunofluorescence test strip component for quickly quantitatively testing troponin-T and test card component manufactured by same and production process of immunofluorescence test strip
  • Immunofluorescence test strip component for quickly quantitatively testing troponin-T and test card component manufactured by same and production process of immunofluorescence test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Refer to attached figure 1 , an immunofluorescence test strip assembly for rapid quantitative detection of troponin T, comprising a test strip and a platinum porphyrin-labeled specific antibody that is used in conjunction with the test strip and is individually packaged, the test strip includes a substrate 1, sequentially bonded Water-absorbing pad 2, coated analysis membrane 3 and sample pad 6 on the substrate 1, detection line 4 and quality control line 5 are arranged on the coating analysis membrane 3, and the specific antibody coated by detection line 4 is anti-sarcocalcin Protein T monoclonal antibody, the specific antibody coated by quality control line 5 is rabbit IgG antibody; the platinum porphyrin-labeled specific antibody is anti-troponin T monoclonal antibody and anti-rabbit IgG antibody.

[0055] Among them, one side of the bottom liner 1 is coated with viscose or double-sided tape to fix the absorbent pad 2, the coated analysis film 3 and the sample pad 6,...

Embodiment 2

[0093] This embodiment is basically the same as Embodiment 1, the difference lies in:

[0094] The method for preparing the detection line coating buffer is: 20mM trimethylolmethaminopropanesulfonic acid buffer (TAPS buffer) at pH7.6, containing 0.8% methanol, 1.5% sucrose, 0.6% bovine serum albumin, anti- cTnT monoclonal antibody 1mg / ml. Preparation of quality control line coating buffer: 50mM pH7.6 phosphate buffer (PB buffer), containing 0.7% methanol, 0.5% bovine serum albumin, and 0.5mg / ml rabbit IgG. Preparation of the coating film: debug the BIO-DOT film spraying machine, the film liquid volume is 20ul / 40cm, the machine is scribed, the distance between the detection line and the quality control line is 5mm, the scribed line is fine and uniform, and it is placed in a vacuum drying oven at 25°C-37°C for 1.5 Hours, bagged and sealed for later use.

Embodiment 3

[0096] This embodiment is basically the same as Embodiment 1, the difference lies in:

[0097] Dilute the anti-cTnT monoclonal antibody and anti-rabbit IgG antibody with 0.1M sodium bicarbonate solution to 1mg / ml respectively, take 5ml of the antibody solution, add 40mg of platinum porphyrin solution, stir well, and incubate at room temperature for 1.5 hours. Mix every 15 minutes. Finally, G25 gel column was used for column separation and purification, and the labeled platinum porphyrin-labeled antibody was collected, and the antibody containing 0.01%-0.5% PEG, 1%-5% bovine serum albumin, 5%-20% glycerol, 0.01%- Dilute with 0.05% surfactant in 0.02M phosphate buffer, seal and pack in plastic bottles, and store at 4°C.

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Abstract

The invention discloses an immunofluorescence test strip component for quickly quantitatively testing troponin-T and a test card component manufactured by the same. The test strip component comprises a test strip and a PT-porphyrin mark specific antibody packed individually. The test strip comprises a bottom liner, an envelope analysis membrane, a sample pad and an absorbent pad, wherein the envelope analysis membrane is attached on the bottom liner, the sample pad is attached on the bottom liner and jointed with one end of a nitrocellulose membrane, the absorbent pad is attached on the bottom liner and jointed with the other end of the nitrocellulose membrane, and the envelope membrane is provided with a test line and a quality control line. Further, the test line envelopes a troponin-T monoclonal antibody, the quality control line envelopes rabbit IgG antibody. The test card is formed by combining a card case with the test strip component. The test strip component and the test card consisting of the same have the advantages of operational simplicity, fastness, flexibility and good specificity and the like when used for testing troponin-T in human blood, serum or plasma, and have good clinical application prospects.

Description

technical field [0001] The invention belongs to the field of medical examination, and in particular relates to an immunofluorescence test strip for rapid and quantitative detection of troponin T (cTnT), as well as its preparation and application. Background technique [0002] Coronary heart disease has become a major disease affecting the health of the population. Acute myocardial infarction is the main cause of death in coronary heart disease patients. How to timely discover patients with acute myocardial infarction and corresponding treatment is the key to saving the lives of patients with myocardial infarction. Traditional diagnosis is mainly based on typical clinical manifestations, ECG changes and laboratory enzyme examination. However, a considerable number of patients with myocardial infarction have no obvious clinical manifestations, and there is no obvious change in the early ECG. In this case, the application of specific markers of myocardial injury plays a key ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/558
Inventor 罗琪谢爱武梁万兴李必松
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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