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Method for regulating pore structure of water-soluble polymer tissue engineering scaffold by use of polyester template

A technology of water-soluble polymers and tissue engineering scaffolds, applied in the fields of single-component polyester artificial filaments, medical science, prostheses, etc., can solve the problems of poor cell adhesion, limited porogens, and lack of cell interaction site and other issues, to achieve the effect of good pore connectivity and controllable pore size

Inactive Publication Date: 2012-09-26
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in previous reports on the preparation of scaffolds, oil-soluble polyester materials were mostly used as raw materials, and polyester materials lack sites for interaction with cells, poor cell adhesion, and clinically found that their acidic degradation products can cause nonspecific Heterosexual aseptic inflammation, and the porogens used in this method are limited to water-soluble particles such as sodium chloride and sugars

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The polylactic acid microspheres were prepared by the emulsion method, and the microspheres with a diameter of 300-350 μm were selected by screening.

[0023] Poly-L-glutamic acid and chitosan were separated by -COOH and -NH 2 The scaffold solution was prepared with a molar ratio of 1:1 and a total solid content of 3%. Add microspheres whose volume accounts for 5% of the scaffold solution volume, and stir well to disperse. The porous scaffold was obtained by freeze-drying, etching, and vacuum drying. The scaffold has continuous spherical holes with a diameter of 300-350 μm; in addition, because the scaffold is prepared by freeze-drying technology, it has many small hole structures with a diameter of 20-50 μm. The measured porosity of the scaffold was 94.49%, and the swelling degree was 793%.

Embodiment 2

[0025] The polylactic acid microspheres were prepared by the emulsion method, and the microspheres with a diameter of 300-350 μm were selected by screening.

[0026] Poly-L-glutamic acid and chitosan were separated by -COOH and -NH 2 The scaffold solution was prepared with a molar ratio of 1:1 and a total solid content of 3%. Add microspheres whose volume accounts for 10% of the scaffold solution volume, and stir well to disperse. The porous scaffold was obtained by freeze-drying, etching, and vacuum drying. The scaffold has continuous spherical holes with a diameter of 300-350 μm; in addition, because the scaffold is prepared by freeze-drying technology, it has many small pores with a diameter of 20-50 μm; The number of spherical holes also increased. The measured porosity of the scaffold was 94.75%, and the swelling degree was 845%.

Embodiment 3

[0028] The polylactic acid microspheres were prepared by the emulsion method, and the microspheres with a diameter of 450-500 μm were selected by screening.

[0029] Poly-L-glutamic acid and chitosan were separated by -COOH and -NH 2 The scaffold solution was prepared with a molar ratio of 1:1 and a total solid content of 3%. Add microspheres whose volume accounts for 5% of the scaffold solution volume, and stir well to disperse. The porous scaffold was obtained by freeze-drying, etching, and vacuum drying. The scaffold has continuous spherical holes with a diameter of 450-500 μm; in addition, because the scaffold is prepared by freeze-drying technology, it has many small hole structures with a diameter of 20-50 μm. The measured porosity of the scaffold was 94.57%, and the swelling degree was 817%.

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Abstract

The invention discloses a method for regulating the pore structure of a water-soluble polymer tissue engineering scaffold by use of a polyester template. The method comprises the following steps of: adding polylactic acid microspheres or fiber into a water-soluble polymer solution; performing freeze drying; and then removing the template to obtain a porous tissue engineering scaffold. The experimental result shows that: the method can obtain a tissue engineering scaffold with aperture of 100-500 microns, over 90% of porosity and over 700% of swelling degree; the scaffold has the characteristics of controllable aperture, sequential arrangement of holes, good pore connectivity and coexistent big and small pores; the structure with coexistent big and small pores is favorable for inoculating and adhering cells, transporting nutrient substances and discharging metabolic products; and moreover, the research indicates that: the differentiation behavior of the stem cell in the scaffold can be intervened in by adjusting the pore structure of the scaffold.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for regulating the pore structure of a water-soluble polymer tissue engineering scaffold with a polyester template. Background technique [0002] For tissue engineering, tissue engineering scaffolds are one of the keys to regeneration of tissue defects. Tissue engineering scaffold is a substitute for extracellular matrix (ECM), which provides physical, chemical and mechanical stimulation signals for cell behaviors such as cell-to-cell interaction, proliferation, differentiation and migration; Regulation and remodeling of the extracellular matrix. Their interactions play important roles in tissue growth, homeostasis, and recovery from disease. [0003] Therefore, tissue engineering scaffolds not only need to simulate the bulk structure and surface physical and chemical properties of natural extracellular matrix (such as chemical composition, hydrophilicity an...

Claims

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Application Information

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IPC IPC(8): C08J9/32C08J9/26C08L77/02C08L5/08A61L27/56C08J3/12C08L67/04D01F6/62
Inventor 尹静波郭石卿孙园园张丹青颜世峰
Owner SHANGHAI UNIV
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