Compound simulating cabin for rat and mice tests and control method thereof

Abstract

The invention provides a compound simulating cabin for rat and mice tests. The compound simulating cabin is characterized by comprising a test cabin body, a nitrogen input device, an oxygen input device, a carbon dioxide input device, a concentration and humidity detecting device, a one-way valve, a mixing fan, a PLC and a host computer, wherein force control configuration software is installed on the host computer; outputs of the nitrogen input device, the oxygen input device and the carbon dioxide input device are all connected with the test cabin body; gases in a certain proportion are introduced into the test cabin body by the nitrogen input device, the oxygen input device and the carbon dioxide input device according to a preset environmental model requirement; the numerical values of the gases in the test cabin body are monitored in real time by the concentration and humidity detecting device; and a preset program of the host computer is used for controlling, thereby controlling the switch-on / off of the nitrogen input device, the oxygen input device and the carbon dioxide input device.

Description

technical field [0001] The present invention relates to an experimental animal breeding chamber, in particular to a composite rat and mouse chamber for simulating intermittent hypoxia at normal pressure, hypoxia at normal pressure and high carbon dioxide, intermittent hypoxia and high carbon dioxide, continuous high oxygen and continuous hypoxia. Test simulation cabin. Background technique [0002] At present, many experiments in medicine often use rats or mice to simulate a certain disease environment for research. Therefore, different types of rat and mouse test simulation cabins appear in the medical field, mainly for the research of the following diseases: [0003] 1) The characteristic hypoxic mode of obstructive sleep apnea-hypopnea syndrome (OSAHS) is chronic intermittent hypoxia, but the specific mechanism has not yet been clarified, so the establishment of animal experimental models is particularly important for the study of the mechanism. There are different types...

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Problems solved by technology

There are different types of experimental devices for establishing the model in the early stage: ① reciprocating hypoxic chamber, which allows the animal chamber to enter the hypoxic environment intermittently, which can basically simulate the pathophysiological characteristics of repeated hypoxia-reoxygenation, but requires high equipment and complicated operation; ②The low-pressure hypoxic chamber simulates different altitudes by changing the ascending and descending rate of the oxygen chamber to generate different pressures and oxygen concentrations, thereby achieving the effect of hypoxia-reoxygenation. Oxygen condition; ③Low O2high CO2 box, using single-chip microcomputer technology and feedback principle to automatically adjust O2 , CO 2 concentration, to establish a model of hypoxia and CO2 retention, the interference of CO2 in the study of hypoxia-reoxygenation mechanism cannot be ruled out; Existing intermittent hypoxic chamber: the gas source uses mixed gas, which is not easy to obtain; nitrogen (or hypoxic mixed gas) and air are used Alternate delivery mode, the oxygen concentration in the large volume cabin is not easy to rise rapidly; too much gas flow will cause noise, and the flow of air flow will have a certain impact on the physiology of animals, all of which increase the interference of the experiment

[0004]2) Chronic obstructive pulmonary disease (COPD) is a major disease of the respiratory system. Pulmonary hypertension caused by retention is an important complication of COPD. In order to study its pathological mechanism, researchers at home and abroad have designed two models of alpine hypoxia and normal pressure hypoxia. The models developed in the past have a low degree of automation. Sampling and analyzing the concentration of oxygen and carbon dioxide requires manual operation, and the work is labor-intensive. The gas concentration, temperature, and humidity in the cabin are easily disturbed by animal breathing and external gases, and are difficult to control; the existing semi-automatic device "low oxygen and high carbon dioxide cultivation cabin", It can only measure the concentration of oxygen and carbon dioxide in the cultivation chamber, and cannot realize the automatic control of various concentrations, nor has the functions of data storage, processing and generating reports

[0007]5) Hypoxic pulmonary hypertension (HPH) is the central link in the pathogenesis of cor pulmonale, and the occurrence and severity of HPH obviously affect the The course and prognosis of cor pulmonale, the pathogenesis of hypoxic pulmonary hypertension is currently believed to be hypoxic pulmonary vasoconstriction and hypoxic pulmonary vascular remodeling, but the specific mechanism is still unclear, in order to further explain the pathogenesis of hypoxic pulmonary hypertension , established a hypoxic model, the previous model was low in automation, gas concentration monitoring required manual operation, and the work was labor-intensive. The gas concentration, temperature, and humidity in the cabin were easily interfered by external gases and were not easy to control

[0008] To be applied to the several environmental models mentioned above, there are many deficiencies in the animal cabins for the preparation of animal models reported in the past, such as poor sealing performance of the cabin body, for Oxygen concentration is controlled by manual detection, and it is difficult to keep the oxygen concentration in the cabin stable, so there are many deficiencies in the preparation of the model

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