Preparation method for high-sensitivity amperometric glucose sensor

Abstract

The invention relates to a preparation method for a high-sensitivity amperometric glucose sensor. Alpha-Ni(OH)2 sol and a nanostructured membrane are first prepared, cut FTO (fluorine-doped tin oxide) electrodes are then ultrasonically washed sequentially with isopropanol and methanol for 15 minutes, and are dried in the air, and under the rotational speed of 2500rpm, the spin-coating method is adopted to spin-coat the prepared Alpha-Ni(OH)2 sol on each electrode within 1.0cm2 of limited area for 2 minutes; finally, the electrode is dried under vacuum for 24 hours and then roasted under 240 DEG C for 30 minutes, and thereby the nanostructured membrane-modified electrochemical sensor formed by Alpha-Ni(OH)2 is obtained. Cyclic voltammetry and flow injection analysis indicate that the sensor shows enlarged surface area and enhanced electrocatalytical property.

Description

Technical field: [0001] The invention relates to a preparation method of a glucose sensor, in particular to a preparation method of a high-sensitivity current type glucose sensor. Background technique: [0002] Glucose is a very important metabolite, and its monitoring is a fundamental problem in the fields of biomedicine, food, beverage and biochemical technology. Near-infrared spectroscopy is commonly used in industry to estimate the concentration of glucose, but this method is very expensive and impractical for routine monitoring, thus promoting the development of simple and inexpensive electrochemical sensors. [0003] Amperometric glucose sensors can be classified into two types: enzymatic and non-enzymatic. For the enzymatic glucose sensor, glucose oxidase is immobilized on the surface of the electrode, and molecular oxygen is reduced to hydrogen peroxide while glucose is oxidized, which is then monitored in the form of electric current. Enzymes are highly selective,...

AI Technical Summary

Problems solved by technology

Enzymes are highly selective, but at the same time have a number of disadvantages: their stability is limited and their reactions are dependent on temperature and pH

[0005] In fact, conventional electrochemically grown materials lose half of their energy-storage capacity after 50 redox processes, but nanostructured electrodes show little significant change in their energy-storage capacity even after 200 such processes.