Kiwi fruit seed oil liposome oral liquid and preparation method thereof
An oil liposome, kiwifruit seed technology, applied in the directions of liposome delivery, blood diseases, pharmaceutical formulations, etc., can solve problems such as loss of kiwifruit seed oil, and achieve the effects of promoting commercial value, facilitating long-term storage, and facilitating transportation
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Embodiment 1
[0035] 1. Determination of Encapsulation Efficiency of Kiwifruit Seed Oil Liposomes
[0036] The content of kiwifruit seed oil was determined by dialysis-ultraviolet spectrophotometry. Pipette 20ml kiwifruit seed oil liposomes into the dialysis bag, after dialysis for 17h, pour the solution in the bag into a 100ml volumetric flask, select ethanol as the demulsifier, combine ultrasound, add sherwood oil to extract, the mixed solution is centrifuged (3000r / min, 3min), take 10ml of supernatant in a 25ml volumetric flask, dilute to volume with petroleum ether, measure its absorbance A at 233nm 1 ; Determination of blank liposome absorbance A with the same method 0 , Calculate △A, calculate the content of encapsulated kiwifruit seed oil from the standard curve.
[0037] Encapsulation rate%=△A*100 / A 1
[0038] 2. Formula and process optimization
[0039] The technical key of liposome oral solution lies in the preparation of liposome. Taking the encapsulation rate as an inde...
Embodiment 2
[0047] Set the constant temperature oscillator temperature to 60 °C and preheat. Accurately measure 100ml of pH 6.8 phosphate buffer solution and place it in a constant temperature shaker, heat it in a water bath to 60°C, and keep it at a constant temperature at a speed of 150r / min. Using absolute ethanol as solvent, respectively prepare lecithin (LC) concentration of 20mg / ml, cholesterol (CH) concentration of 10mg / ml, kiwifruit seed oil (KFO) concentration of 15mg / ml, purity 99.0%. After the solutions were mixed evenly, they were uniformly injected into the phosphate buffer solution in the constant temperature shaker with a syringe. 60°C constant temperature water bath for 30 minutes, remove residual ethanol, ultrasonic treatment for 30 minutes (ultrasonic power 80W), add distilled water to make up to 250ml, seal, irradiate and sterilize to obtain kiwifruit seed oil liposome oral solution.
[0048] The method of the present invention preferably adopts kiwifruit seed oil prov...
Embodiment 3
[0050] Using ethanol as a solvent, respectively prepare lecithin (LC) concentration of 16 mg / ml, cholesterol (CH) concentration of 12 mg / ml, kiwifruit seed oil (KFO) concentration of 10 mg / ml, the preparation method is the same as that of Example 2.
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