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Process for using intestine casing to extract sodium heparin

A heparin sodium production process technology, which is applied in the field of heparin sodium process, can solve the problems of large residual amount and incomplete decomposition, and achieve the effects of saving extraction time, improving extraction rate and reducing production cycle

Active Publication Date: 2012-08-15
RUGAO YONGXING CASING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this process is: the decomposition is not complete, and the residual amount is relatively large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The vibration time of the vibrating enzymolysis machine is 20 minutes, the transmission power of the ultrasonic machine is 240W, the total duration is 7 minutes, and the duration of a single ultrasonic wave emission is 10S. The yield of heparin sodium in a single small intestine is

[0013] 0.61 mg.

Embodiment 2

[0015] The vibration time of the vibrating enzymolysis machine is 29 minutes, the transmission power of the ultrasonic machine is 255W, the total duration is 8 minutes, and the duration of a single ultrasonic wave emission is 11S. The yield of heparin sodium in a single small intestine is

[0016] 0.63 mg.

Embodiment 3

[0018] The vibration time of the vibrating enzymolysis machine is 35min, the transmission power of the ultrasonic machine is 270W, the total duration is 9min, and the duration of a single ultrasonic wave emission is 12S. The yield of heparin sodium in a single small intestine is

[0019] 0.67 mg.

[0020] 3. Ion exchange adsorption: first cool the above enzymolysis extract to room temperature, carefully remove the thin layer of grease floating on the liquid surface, control the temperature to 45 degrees, stop heating, add the pre-treated D-254 under stirring The new resin has effectively adsorbed the heparin sodium component in the feed liquid. The dosage of new resin is generally 2.55-3% of the feed liquid. After use, the regeneration should be increased as appropriate. After stirring and adsorption for 3 hours, let it stand Filtrate, the filtrate can be used as a raw material for the production of new drug Guanxinshu for the treatment of coronary heart disease.

[0021] Ri...

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Abstract

The invention relates to a process for using an intestine casing to extract sodium heparin. The process provided by the invention mainly comprises the following steps: material processing, enzymatic extraction, adsorption, desorption, precipitation and oven-drying. The innovative points provided by the invention comprise: mixed liquor of mucous membrane of small intestine and feed liquid is firstly put in a vibrating enzymolysis machine; protease is added in the vibrating enzymolysis machine for vibrating enzymolysis; the vibration frequency and the vibration time of the vibrating enzymolysis machine are 250HZ and 20-35min, respectively; then the mixed liquor is put in an ultrasound machine for further enzymolysis; and the ultrasound machine has an ultrasonic wave transmitting power at the range of 240-270W, a total ultrasonic wave transmitting duration time at the range of 7-9min and a single ultrasonic wave transmitting duration time at the range of 10-12S. According to the invention, the extraction process is adopted to extract sodium heparin, so as to increase the extraction volume by 18-23% compared with a single enzymatic method, increase the extraction volume by 13-16% and save the extraction time by 30-72min compared with a single ultrasonic wave method. The process provided by the invention has high extraction ratio and reduces the production cycle.

Description

technical field [0001] The invention relates to a heparin sodium process, in particular to a process for extracting heparin sodium from casings. Background technique [0002] Heparin sodium is a mucopolysaccharide sulfate anticoagulant. Heparin sodium is the sodium salt of aminodextran sulfate extracted from the intestinal mucosa of pigs or cattle, and belongs to mucopolysaccharides. Crude heparin sodium is an important raw material for the manufacture of heparin sodium drugs. The preparation process is: after enzymatic hydrolysis, adsorption, desorption, precipitation, and final drying of the small intestinal mucosa of animals, the crude heparin sodium can be obtained. In the prior art, there are two extraction techniques for heparin sodium in animal casings: salt hydrolysis extraction, enzymatic hydrolysis extraction, and enzymatic hydrolysis plus salt hydrolysis extraction. The disadvantage of this process is that the decomposition is not complete and the residue is re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 费苹费正海陈银芳费正明陈明全陈跃建
Owner RUGAO YONGXING CASING
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