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Preparation method of Zanthoxylum dissitum Hemsl skin polysaccharide and application

A technology of shell pepper and fruit peel, which is applied in the field of medicine to achieve good anti-tumor effect and simple method

Inactive Publication Date: 2012-08-08
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on Zanthoxylum bungeanum is mainly based on basic research, such as chemical composition analysis, tissue culture technology and biological characteristics. It is reported that the neutral lipophilic components of Zanthoxylum bungeanum have inhibitory effects on Staphylococcus aureus, Salmonella and Escherichia coli. However, there have been no literature reports or patent publications on the extraction method and anti-tumor activity of Zanthoxylum bungeanum polysaccharides.

Method used

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  • Preparation method of Zanthoxylum dissitum Hemsl skin polysaccharide and application
  • Preparation method of Zanthoxylum dissitum Hemsl skin polysaccharide and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Extraction of polysaccharides from shell pepper pericarp

[0016] (1) Pulverization: pulverize the dry shell pepper peel into 40-60 mesh powder with a pulverizer;

[0017] (2) Degreasing: put the powder in the extractor, degrease twice with petroleum ether, filter for 1 hour each time, obtain the filter residue, then degrease twice with 95% ethanol, 2 hours each time, filter, and volatilize to remove the organic matter in the filter residue. solvent;

[0018] (3) Extraction: According to the ratio of distilled water volume mL to filter residue weight g of 10 to 20:1, add distilled water to the filter residue, extract at 100°C for 2 to 3 hours, repeat this step three times, and combine the three extracts;

[0019] (4) Protein removal: remove protein by enzymatic hydrolysis, centrifuge to obtain supernatant;

[0020] (5) Concentration: Concentrate the supernatant under reduced pressure at 50°C to 80°C to obtain a concentrate;

[0021] (6) Alcohol analysis...

Embodiment 2

[0022] Embodiment 2: Extraction of polysaccharides from shell pepper pericarp

[0023] (1) Pulverization: pulverize the dry shell pepper peel into 40-60 mesh powder with a pulverizer;

[0024] (2) Degreasing: put the powder in the extractor, degrease twice with petroleum ether, filter for 1 hour each time, obtain the filter residue, then degrease twice with 95% ethanol, 2 hours each time, filter, and volatilize to remove the organic matter in the filter residue. solvent;

[0025] (3) Extraction: According to the ratio of distilled water volume mL to filter residue weight g of 10 to 20:1, add distilled water to the filter residue, extract at 100°C for 2 to 3 hours, repeat this step three times, and combine the three extracts;

[0026] (4) Protein removal: use Sevag method to remove protein, centrifuge to obtain supernatant;

[0027] (5) Concentration: Concentrate the supernatant under reduced pressure at 50°C to 80°C to obtain a concentrate;

[0028] (6) Alcohol analysis: ad...

Embodiment 3

[0029] Embodiment 3: phenol sulfuric acid method measures polysaccharide content

[0030] (1) Preparation of standard solution: Accurately weigh 0.1000 g of glucose reference substance dried at 105°C to constant weight, add distilled water to dissolve, set the volume in a 250mL volumetric flask, make 0.4mg / mL glucose standard solution, and accurately draw the standard solution Liquids 1, 2, 3, 4, and 5 mL were placed in 10 mL volumetric flasks, and shaken to volume with distilled water to obtain a series of reference substance solutions.

[0031] (2) Drawing of the standard curve: Precisely draw 1.0mL of the above-mentioned series of reference substance solutions, place them in 10mL colorimetric tubes, place the colorimetric tubes in ice water, add 1mL of phenol reagent to the colorimetric tubes, and then add concentrated sulfuric acid 4mL, shake well at the same time after each tube is added, place in boiling water to heat for 10min, cool to a constant volume and measure the ...

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Abstract

Disclosed are a preparation method of Zanthoxylum dissitum Hemsl skin polysaccharide and an application. The preparation method comprises the following steps of: (1) crushing dry Zanthoxylum dissitum Hemsl skin into a powder of 40-60 meshes by the use of a pulverizer; (2) placing into an extractor, degreasing by an organic solvent, repeating the step twice, and filtering to obtain filter residue after degreasing; (3) according to the ratio of the volume (mL) of distilled water to the weight (g) of the filter residue being 10-20:1, adding distilled water into the filter residue, extracting at 100 DEG C for 2-3h, repeating the step for three times, and merging the three extracts; (4) removing protein by Sevag method or enzymatic hydrolysis method, and centrifuging to obtain a supernatant; (5) carrying out vacuum concentration on the supernatant at the temperature of 50-80 DEG C, and making the volumn (mL) of the solution 4-6 times of the weight (g) of the Zanthoxylum dissitum Hemsl powder from the Step (1) to obtain a concentrate; and (6) adding ethanol into the concentrate until the alcohol concentration is 80%, standing overnight, centrifuging to obtain a precipitate, and drying the precipitate to obtain Zanthoxylum dissitum Hemsl skin polysaccharide. The Zanthoxylum dissitum Hemsl skin polysaccharide can be used to prepare medicines for alleviating and treating cancer.

Description

technical field [0001] The invention relates to the technical field of medicine. Background technique [0002] Tumor is one of the main diseases that endanger human health at present. Research on anti-tumor drugs at home and abroad has found that chemical anti-cancer drugs endanger normal cells while acting on target cells. Most of the chemical drugs used to treat tumors in clinical practice will cause mutational genotoxicity, but the mutational genotoxicity of Chinese herbal medicines is not obvious. The anti-cancer active ingredients of Chinese herbal medicine mainly include saponins, polysaccharides, alkaloids and other components. Among them, the anti-tumor mechanism of polysaccharides mainly includes direct anti-tumor mechanism based on cytotoxicity and indirect anti-tumor mechanism based on immune effect. It has become the current anti-tumor mechanism. One of the main directions for the development of new tumor drugs. [0003] Shell pepper (Zanthoxylum Dissitum Hemsl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/758C08B37/00A61P35/00A61K131/00
Inventor 杨林邵文斌于爱红姚广玉
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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