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Sophora fungus mycoplasma extract identification and detection method

An identification method and technology of Huaier fungus, applied in the field of analysis and detection, can solve problems such as insufficient control of polysaccharide and protein components.

Active Publication Date: 2012-08-01
QIDONG GAITIANLI MEDICINES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various technical bottlenecks in the detection of sugars, the current quality standards of Huaier fungus and its related products measure the total protein content. For polysaccharides, only glucose is used as a control for preliminary determination. Insufficient to effectively control polysaccharide and protein components in Huaier fungus extract

Method used

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  • Sophora fungus mycoplasma extract identification and detection method
  • Sophora fungus mycoplasma extract identification and detection method
  • Sophora fungus mycoplasma extract identification and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] 1. Detection standard of Huaier Qing Gao polysaccharide protein component monosaccharide composition fingerprint

[0084] 1. Chromatographic conditions and system suitability test

[0085] Styryl divinyl benzene packing phase with alkyl quaternary ammonium salt functional groups, eluted with 1mM NaOH solution (generated by an online eluent generator), flow rate 0.5mL / min, column temperature 30°C, electrochemical pulse ampere For the detector, the number of theoretical plates shall not be less than 3000 based on the arabinose peak.

[0086] 2. Preparation of reference substance solution, take arabinose, glucose, galactose, xylose, mannose reference substance appropriate amount, add deionized water to prepare 10.0mg / L solution respectively as reference substance solution.

[0087] 3. Preparation of the test solution: Accurately weigh 5.0g of Huaierqing paste powder, add 100mL of water to dissolve, then add 400mL of absolute ethanol, let it stand for 2 hours, centrifuge, ...

Embodiment 2

[0144] 1. Detection standard of Huaier granule polysaccharide protein component monosaccharide composition fingerprint

[0145] 1. Chromatographic conditions and system suitability test

[0146] Styryl divinylbenzene packing phase with alkyl quaternary ammonium salt functional groups, eluted with 1mM NaOH solution (generated by an online eluent generator), flow rate 0.5mL / min, column temperature 30°C, electrochemical detector , sugar standard four-potential waveform detection mode, the number of theoretical plates calculated by the arabinose peak, should not be less than 3000.

[0147] 2. Preparation of reference solution

[0148] Take appropriate amount of reference substances of arabinose, galactose, xylose and mannose, dissolve them with deionized water respectively to form a 10.0 mg / L reference solution, shake well, and obtain.

[0149] 3. Preparation of the test solution

[0150] Accurately weigh the contents of 5 bags of Huaier granules, grind them finely, accurately ...

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Abstract

The invention relates to a sophora fungus mycoplasma extract identification and detection method. The identification method is characterized in that an anion exchange chromatography method is used for establishing chromatographic fingerprint composed by monosaccharide of proteoglycan protein in the sophora fungus mycoplasma extract, and the quality is controlled by comparing the similarity of a sample fingerprint spectrum and a fingerprint spectrum of a standard extract. The detection method is characterized in that the anion exchange chromatography method is used for detecting the monosaccharide composition and content of proteoglycan protein of the sophora fungus mycoplasma extract, thereby the content limit can be made. The fingerprint spectrum of the anion exchange chromatography method combines the content determination method to realize the effective control of quality of the sophora fungus mycoplasma extract and the relative products as well as distinguish with other products. The method of the invention has the advantages of rapidity, convenience and strong specificity.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to an identification and detection method of Huaier fungus extract. Background technique [0002] Huaier (Trametes robiniophila Murr.), scientific name Trametes robiniophila, is an important folk medicinal fungus. It has a history of more than 1,600 years of medicinal use in China. The Ainsworth fungal classification system attributes it to Fungi, Basidiomycotina, and Phytobacteria Class, Polyporaceae, Trametes. The fruiting body of Huai Er is medium to large, corky, sterile, and is born on the trunks of broad-leaved trees such as Sophora japonica and Black locust. It is distributed in Hebei, Shaanxi, Liaoning, Hunan, Guangxi, Fujian and other places. It is a kind of tree that can cause Heartwood rot fungi. The taste is bitter and pungent, and its nature is flat and non-toxic. It has the effects of "curing wind", "breaking blood" and "reinforcing strength". It is ...

Claims

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Application Information

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IPC IPC(8): G01N30/66G01N30/06
Inventor 徐无为陆正鑫
Owner QIDONG GAITIANLI MEDICINES
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