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Method for preparing liquid strain of chanterelle

A technology for liquid strains and chanterelles, which is applied in the field of preparation of liquid strains of chanterelles, can solve the problems of high pollution rate, long growth period, complicated process and the like, and achieves the effects of simple operation, less pollution and simple process.

Inactive Publication Date: 2012-08-01
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome defects such as the development and utilization of existing chanterelles relying on wild resources, the high pollution rate of existing strains, long growth cycle, and complicated process, and provide a liquid strain of chanterelles The preparation method has the advantages of simple process, pure strain, less pollution and short cycle. The liquid strain can be used to imitate wild cultivated chanterelles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Medium formula: 200g pine needles, 200g potatoes, 0.1g calcium chloride, 0.5g ammonium chloride, 0.3g magnesium sulfate, 1g potassium dihydrogen phosphate, 0.01g ferric chloride, 0.5g glucose, vitamin B 1 0.05 mg, peptone 5.0g, beef extract 10g, urea 20g, wheat grain 10g, pH value 5.5, water to 1000 ml.

[0016] (1) Collection of chanterelles: from May to September when the fruiting bodies of chanterelles are mature;

[0017] (2) Cleaning and removing impurities: clean the soil and other impurities attached to the chanterelle fruit bodies with alcohol cotton, and put the cleaned fruit bodies into the fresh-keeping layer of the refrigerator or directly proceed to the next process;

[0018] (3) Medium preparation: Wash the pine needles, cut the potatoes into small pieces, put them in a pot, add 1000ml of water, boil for 30 minutes, filter out the pine needles and potato residues with gauze, put the filtrate back into the pot, add calcium chloride, chlorine Ammonium chlo...

Embodiment 2

[0023] Medium formula: 150g pine needles, 250g potatoes, 0.05g calcium chloride, 0.55g ammonium chloride, 0.35g magnesium sulfate, 0.8g potassium dihydrogen phosphate, 0.008g ferric chloride, 0.45g glucose, vitamin B 1 0.045 mg, peptone 6g, beef extract 12g, urea 15g, wheat grain 8g, pH value 5.5, water to 1000 ml.

[0024] (1) Culture medium preparation: Wash the pine needles, cut the potatoes into small pieces, put them in a pot, add 1000ml of water, boil for 20 minutes, filter out the pine needles and potato residues with gauze, put the filtrate back into the pot, add calcium chloride, chlorine Ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate, ferric chloride, glucose, vitamin B 1 , peptone, beef extract, urea and wheat grains, set the volume to 1000ml, pack them in Erlenmeyer flasks, 200ml per bottle, cover with cotton plugs, sterilize with high-pressure steam at 0.1Mpa, 125°C for 25min, and place at 35 Cultivate in an incubator at ℃ for 24 hours, and...

Embodiment 3

[0029] Medium formula: 250g pine needles, 150g potatoes, 0.15g calcium chloride, 0.45g ammonium chloride, 0.25g magnesium sulfate, 1.2g potassium dihydrogen phosphate, 0.012g ferric chloride, 0.55g glucose, vitamin B 1 0.055 mg, peptone 4g, beef extract 8g, urea 25g, pH value 5.5, water to 1000 ml.

[0030] (1) Culture medium preparation: Wash the pine needles, cut potatoes into small pieces, put them in a pot, add 1000ml of water, boil for 40 minutes, filter out the pine needles and potato residues with gauze, put the filtrate back into the pot, add calcium chloride, chlorine Ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate, ferric chloride, glucose, vitamin B 1 , peptone, beef extract, urea and wheat grains, set the volume to 1000ml, pack them in Erlenmeyer flasks, 200ml per bottle, cover with cotton plugs, sterilize with high-pressure steam at 0.15Mpa and 118°C for 35min, and place at 25 Cultivate in an incubator at ℃ for 24 hours, and keep the aseptic...

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Abstract

The invention discloses a method for preparing a liquid strain of chanterelle. The method comprises the following steps of: preparing a culture medium; inoculating chanterelle tissue at the joint of a cap and a stem of chanterelle; and culturing in a shaker. Compared with the prior art, the method has the advantages that: the chanterelle tissue is inoculated in a unique culture medium and cultured in the shaker, the process is simple and convenient, pollution is light, the strain is pure, the operation is simple, and a culture period is short (only 7-10 days); the obtained liquid strain is inoculated in a cultivation bag to produce chanterelle through bionics wild cultivation, and large-scale industrial production can be realized; and moreover, wheat grains can be added into the culture medium, mycelia are attached to the peripheries of the wheat grains in the shaker culture process, so that the culture time can be effectively shortened further.

Description

technical field [0001] The invention relates to the technical field of producing edible fungus strains, in particular to a method for preparing liquid chanterelles strains. Background technique [0002] Chanterelles ( Cantharelles cibarius Fr. ) is one of the world's famous edible fungi. The fruiting body is fleshy, trumpet-shaped, apricot-yellow to egg-yellow, and the flesh is egg-yellow, with a strong aroma and almond flavor. The cap is 3-9cm in diameter, initially flat and then concave, with wavy edges, often cracked and involuted. The stipe is solid and smooth, 2-6cm long and 0.5-1.8cm in diameter. Chanterelles are rich in carotene, vitamin C, protein, calcium, phosphorus, iron and other nutrients. Regular consumption of this fungus can prevent vision loss, ophthalmia, dry skin and other diseases. In addition, according to foreign clinical verification, chanterelles also have certain anticancer activity, and have a certain inhibitory effect on the growth and spread...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 贺红早任春光孙超
Owner GUIZHOU INST OF BIOLOGY
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