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Culture medium of single-cell microalgae and method for treating lead-containing wastewater by using single-cell microalgae

A single-cell algae and culture medium technology, which is applied in the direction of single-cell algae, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of poor treatment effect, achieve the effect of green environmental protection and reduce lead pollution

Active Publication Date: 2013-06-19
GUANGZHOU EP ENVIROMENTAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These biological methods are mainly for heavy metal copper, zinc, nickel, cadmium sewage, and the treatment effect is often not good.

Method used

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  • Culture medium of single-cell microalgae and method for treating lead-containing wastewater by using single-cell microalgae
  • Culture medium of single-cell microalgae and method for treating lead-containing wastewater by using single-cell microalgae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Cultivate S. qingdao in the medium shown in Table 1 (closed culture), the inoculation density is 5 million cells / ml, and the culture temperature is 25°C. After 5 days of culture, centrifuge and concentrate 10 times to obtain unicellular algae Concentrate, transfer the concentrate to the prepared 0.2% agar semi-solid suspension, and mix well. Inject lead-containing wastewater (Pb in wastewater 2+ The concentration is 80mg / L), after 10 days, inoculate rotifers with a density of 3 / ml, and after another 10 days, filter the water body with a 200-mesh sieve to collect zooplankton, and the water can be discharged directly.

[0037]

[0038] After testing, after treatment, the lead content in wastewater was reduced by 72.3%.

Embodiment 2

[0040] Cultivate Sclerophyllum qingdao in the medium shown in Table 2 (closed culture), the inoculation density is 5 million cells / ml, and the culture temperature is 25°C. After 5 days of culture, centrifuge and concentrate 10 times to obtain unicellular algae Concentrate, transfer the concentrate to the prepared 0.7% agar semi-solid suspension, and mix well. Inject lead-containing wastewater (Pb in wastewater 2+ The concentration is 80mg / L), after 10 days, inoculate rotifers with a density of 3 / ml, and after another 10 days, filter the water body with a 200-mesh sieve to collect zooplankton, and the water can be discharged directly.

[0041]

[0042] After testing, the lead content in the wastewater was reduced by 57.4% after treatment.

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Abstract

The invention discloses a culture medium of single-cell microalgae. The culture medium comprises a carbon source, a nitrogen source, a phosphor source and inorganic salts. A method for treating lead-containing wastewater by using the single-cell microalgae comprises the following steps of: firstly, feeding the single-cell microalgae into the culture medium for closed culture; secondly, after the culture is finished, condensing to obtain a concentrated solution of the single-cell microalgae; thirdly, uniformly mixing the concentrated solution with 0.2-0.7 percent agar semisolid suspension and then adding the lead-containing wastewater; fourthly, automatically treating the wastewater by using the single-cell microalgae and placing zooplankton into the wastewater for feeding the single-cell microalgae; and fifthly, collecting the zooplankton and separating water. According to the method for treating the lead-containing wastewater, lead pollution in a water body can be greatly reduced; and meanwhile, the treatment method is environment-friendly, low in price and feasible.

Description

technical field [0001] The invention relates to a culture medium of unicellular algae and a method for treating lead-containing wastewater by using unicellular algae. Background technique [0002] With the rapid development of the economy, a large amount of waste water is discharged, the accumulation of heavy metals in soil and water sources is intensified, and the pollution of heavy metals is also becoming more and more serious. Because heavy metals are easy to bioaccumulate through the food chain, they pose a serious threat to organisms and human health. How to effectively control heavy metal pollution has become a common concern of mankind. Scholars at home and abroad have done a lot of research on the treatment of heavy metal pollution. The wastewater treatment methods that have been developed and applied mainly include chemical methods, physical chemical methods and biological methods, including chemical precipitation, electrolysis, ion exchange, membrane separation, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32C02F101/20
Inventor 吴早春罗丽
Owner GUANGZHOU EP ENVIROMENTAL ENG
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