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Intelligent gel three-dimensional scaffold material for cell culture

A smart gel and three-dimensional scaffold technology, which is applied in tissue culture, biochemical equipment and methods, microorganisms, etc., can solve the problems of lack of biological interaction, difficult purification of toxic compounds, weak water absorption and swelling ability, etc. It is easy to achieve industrialization , low cost, fast degradation effect

Inactive Publication Date: 2012-07-18
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is difficult to purify toxic compounds such as residual initiators in the polymer preparation process, which has a great toxic effect on cells.
In addition, because the polymer molecules are entangled with each other through covalent bonds to form a three-dimensional network structure, the pore size is not easy to adjust, and the microscopic pore size cannot meet the microenvironment required for normal cell growth, and most polymers have poor hydrophilicity. , the water content of the scaffold material formed is often much lower than that of biological tissue, resulting in the lack of biological interaction between the scaffold material and cells
[0004] Because the interaction between low-molecular-weight hydrogels is a non-covalent bond, the pore size is easy to adjust, and the molecules are easy to form gels. The water content of the formed gels can be as high as 99%, but low-molecular-weight hydrogels are also limited by Weak interaction force, the formed gel is often brittle, and the ability to absorb water and swell after drying into a film is weak

Method used

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  • Intelligent gel three-dimensional scaffold material for cell culture
  • Intelligent gel three-dimensional scaffold material for cell culture
  • Intelligent gel three-dimensional scaffold material for cell culture

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preparation example Construction

[0037] The preparation method of the supramolecular gel factor adopted in the present invention is as follows: first synthesize a hydrophobic benzene ring as the core, and insert amino acids such as phenylalanine symmetrically at the 1 and 4 positions of the benzene ring, and have functions such as ethylene glycol. group, the general structure of which is shown in the following formula:

[0038]

[0039] Among them, R 1 Be the structure shown in formula (II), formula (III) or formula (IV):

[0040]

[0041] R 2 Be the structure shown in formula (V), formula (VI), formula (VII) or formula (VIII):

[0042]

Embodiment 1

[0057] Embodiment 1, preparation smart gel support material

[0058] This type of smart gel scaffold material can be prepared by adjusting the temperature of the solvent. The preparation process is as follows: figure 2 shown.

[0059] Firstly, sodium hyaluronate was dissolved in water to prepare a 1wt% sodium hyaluronate solution, and then the aforementioned supramolecular gel factor A was added to the above sodium hyaluronate solution at a concentration of 2.5 mg / ml. Heat (heating temperature 80°C, heating time 5min) the sample until the gel factor is completely dissolved to form a mixed solution, then cool to room temperature, cooling time is 10min, the gel is formed. The gel is dried (drying temperature is 65° C., time is 12 hours) until the moisture is completely evaporated to form a transparent film-xerogel, which is the intelligent gel scaffold material.

[0060] The nanofibers that make up the gel were characterized separately by scanning microscopy, as image 3 s...

Embodiment 2

[0063] Embodiment 2, two-dimensional cell culture experiment

[0064] First, a mixed gel solution containing 0.05wt% gelatin factor A and 0.2wt% natural polysaccharide (the sample was prepared by dissolving the gelatin factor by heating in an aqueous solution of natural polysaccharide and then cooling to 20°C for 30 minutes) was added to the 24-well polystyrene Place the ethylene cell culture plate in an ethanol-sterilized vacuum oven at 70°C and dry for 12 hours to obtain a cell culture plate with a surface modified by the mixed gel. Use DMEM medium (containing 10% fetal bovine serum in the medium) to cultivate human skin fibroblasts (NHSF), under the condition of 37 ℃, feed carbon dioxide so that the volume concentration of carbon dioxide in the culture environment is 5%, and cultivate for 3 days. It was found that the above cells observed the cell morphology in 24 hours, all adhered and spread on the surface of the material, and the growth morphology was good ( Figure 5...

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Abstract

The invention relates to an intelligent gel three-dimensional scaffold material for cell culture, wherein the intelligent gel three-dimensional scaffold material is prepared by the following methods: placing a supramolecular gel factor into water solution of natural polysaccharide, heating the supramolecular gel factor until the supramolecular gel factor is totally dissolved to form mixing solution, also cooling to form gel; and drying the prepared gel until the moisture is totally evaporated and forming a transparent film. The intelligent gel disclosed by the invention concretely is the gel with high water content and high swelling ratio formed by self-assembled fiber of the supramolecular gel factor and natural polysaccharide fiber interpenetrating with each other, and has good biocompatibility and cellular affinity. The cell is facilitated to be adhered, spread and multiplied on the surface of a biological material. The intelligent gel is simple in preparation method, easy in industrial implementation, low in cost and convenient in transportation, and has no special requirements to synthesis equipment, and rigorous and complicated conditions for traditional preparation and purification of polymer gel are avoided.

Description

technical field [0001] The invention relates to the biological technical field of providing cell culture support materials; in particular, it relates to a kind of intelligent gel three-dimensional support materials for cell culture used in tissue engineering. Background technique [0002] The core of tissue engineering is to establish a complex composed of cells and biological materials to form a living tissue with vitality, reconstruct the shape, structure and function of the diseased tissue and achieve permanent replacement; After amplification, large tissue defects are repaired; it can be shaped arbitrarily according to the defects of tissues and organs to achieve perfect shape repair. [0003] At present, the commonly used tissue engineering scaffold materials mainly include natural matrix materials, such as collagen, fibrin, alginate, hyaluronic acid, chitosan, etc. Although they have good biocompatibility, the source is limited and subject to the material's own factor...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08J3/075C08L5/08C08L5/04C08K5/20C12N5/00
Inventor 冯传良李萍张荻
Owner SHANGHAI JIAO TONG UNIV
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