Streptosporangium roseum trehalose synzyme gene and application thereof

Abstract

The invention relates to a Streptosporangium roseum trehalose synzyme gene and an application thereof. The gene is cloned from Streptosporangium roseum, and the expressed trehalose synzyme is capable of converting maltose into trehalose. The most important characteristic of the gene is as follows: the expressed trehalose synzyme has wide range of reaction temperature and pH, can maintain higher enzyme activity at temperature of 25-35 DEG C and pH of 6.5-7.5, and has high conversion efficiency and reaction rate; the content of trehalose in the reaction product produced by converting maltose into trehalose under an optimum reaction condition can reach 82 percent, and little glucose is produced, thereby the conversion efficiency of the substrate is improved, and the production cost is reduced. By application of the recombinant trehalose synzyme produced by the gene, trehalose can be prepared by taking commercial maltose syrup or maltose syrup prepared from starch as material, and the trehalose can be applied to fields of food industries, cosmetics, medicines, biological products and the like.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and modern enzyme engineering, in particular to a Streptomyces rosea trehalose synthase gene and its application. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two molecules of glucose connected by α, α-1, 1 glycosidic bonds. It widely exists in microorganisms, shrimps, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. Exogenous trehalose also has a good non-specific protective effect on organisms, biological macromolecules, and biofilms. It can protect cells from damage caused by environmental changes (such as dehydration, hypertonicity), and the specific performance is Effective protection of biofilm, protein and DNA, etc., so it is known as "sugar of life". The content of trehalose in nature is very low. In the past, it was mainly extracted from dry yeast. Due to the low content, the extraction process is complicated ...

AI Technical Summary

Problems solved by technology

[0004] At present, many literatures and patents have reported the cloning and isolation of genes related to trehalose synthesis, and the use of related enzymes of these trehalose synthase genes to prepare trehalose, but the synthesis pathways of these enzymes related to trehalose synthesis are different, or bacteria Different sources lead to very different DNA sequences and amino acid sequences, the molecular weight of enzymes, enzymatic properties, and the conversion rate of enzymes to substrates, and they have not yet been applied on an industrial scale.

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